C6EID5 (C6EID5_ECOBD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malate synthase G RuleBase RU003572 HAMAP MF_00641
EC=2.3.3.9 RuleBase RU003572 HAMAP MF_00641

Gene names

Name:	glcB HAMAP MF_00641 EMBL ACT44648.1
Ordered Locus Names:	B21_02794, ECBD_0763, ECD_02844

Organism

Escherichia coli (strain B / BL21-DE3) [Complete proteome] [HAMAP]

Taxonomic identifier

469008 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. SAAS SAAS006253 RuleBase RU003572 HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. SAAS SAAS006253 RuleBase RU003572 HAMAP MF_00641
Subunit structure	Monomer By similarity. SAAS SAAS006253 HAMAP MF_00641
Subcellular location	Cytoplasm By similarity RuleBase RU003572 HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily. RuleBase RU003572 HAMAP MF_00641

Ontologies

Keywords
Biological process	Glyoxylate bypass SAAS SAAS006253 RuleBase RU003572 HAMAP MF_00641 Tricarboxylic acid cycle SAAS SAAS006253 RuleBase RU003572 HAMAP MF_00641
Cellular component	Cytoplasm SAAS SAAS006253 HAMAP MF_00641
Molecular function	Acyltransferase EMBL ACT27832.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C6EID5 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 820F177D3FE02632	FASTA	723	80,489
	10 20 30 40 50 60 MSQTITQSRL RIDANFKRFV DEEVLPGTGL DAAAFWRNFD EIVHDLAPEN RQLLAERDRI 70 80 90 100 110 120 QAALDEWHRS NPGPVKDKAA YKSFLRELGY LVPQPERVTV ETTGIDSEIT SQAGPQLVVP 130 140 150 160 170 180 AMNARYALNA ANARWGSLYD ALYGSDIIPQ EGAMVSGYDP QRGEQVIAWV RRFLDESLPL 190 200 210 220 230 240 ENGSYQDVVA FKVVDKQLRI QLKNGKETTL RTPAQFVGYR GDAAAPTCIL LKNNGLHIEL 250 260 270 280 290 300 QIDANGRIGK DDPAHINDVI VEAAISTILD CEDSVAAVDA EDKILLYRNL LGLMQGTLQE 310 320 330 340 350 360 KMEKNGRQIV RKLNDDRHYT AADGSEISLH GRSLLFIRNV GHLMTIPVIW DSEGNEIPEG 370 380 390 400 410 420 ILDGVMTGAI ALYDLKVQKN SRTGSVYIVK PKMHGPQEVA FANKLFTRIE TMLGMAPNTL 430 440 450 460 470 480 KMGIMDEERR TSLNLRSCIA QARNRVAFIN TGFLDRTGDE MHSVMEAGPM LRKNQMKSTP 490 500 510 520 530 540 WIKAYERNNV LSGLFCGLRG KAQIGKGMWA MPDLMADMYS QKGDQLRAGA NTAWVPSPTA 550 560 570 580 590 600 ATLHALHYHQ TNVQSVQANI AQTEFNAEFE PLLDDLLTIP VAENANWSAQ EIQQELDNNV 610 620 630 640 650 660 QGILGYVVRW VEQGIGCSKV PDIHNVALME DRATLRISSQ HIANWLRHGI LTKEQVQASL 670 680 690 700 710 720 ENMAKVVDQQ NAGDPAYRPM AGNFANSCAF KAASDLIFLG VKQPNGYTEP LLHAWRLREK ESH

References

[1]	"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed: 19786035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Korea].
[2]	"Sequencing and gene expression analysis of Escherichia coli BL21." Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Austria].
[3]	"Complete sequence of Escherichia coli BL21(DE3)." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [JGI].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001665 Genomic DNA. Translation: ACT27832.1. CP001509 Genomic DNA. Translation: ACT44648.1. AM946981 Genomic DNA. Translation: CAQ33311.1.
3D structure databases
ProteinModelPortal	C6EID5.
SMR	C6EID5. Positions 1-723.
ModBase	Search...
Protein-protein interaction databases
STRING	C6EID5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000159342; EBESCP00000148144; EBESCG00000158845. EBESCT00000189752; EBESCP00000176324; EBESCG00000186535. EBESCT00000195840; EBESCP00000179506; EBESCG00000190669.
GenomeReviews	Gene locus B21_02794 in contig AM946981_GR. Gene locus ECD_02844 in contig CP001509_GR. Gene locus ECBD_0763 in contig CP001665_GR.
KEGG	ebd:ECBD_0763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010684.
OMA	ENANWSV.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6EID5_ECOBD

Accession

Primary (citable) accession number: C6EID5
Secondary accession number(s): C5W8T0

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)