C6EGS8 (C6EGS8_ECOBD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit HAMAP MF_01202
EC=1.4.99.1 HAMAP MF_01202

Gene names

Name:	dadA HAMAP MF_01202 EMBL ACT43056.1
Ordered Locus Names:	B21_01174, ECBD_2433, ECD_01164

Organism

Escherichia coli (strain B / BL21-DE3) [Complete proteome] [HAMAP]

Taxonomic identifier

469008 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. SAAS SAAS023080 HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. SAAS SAAS023080 HAMAP MF_01202
Cofactor	FAD By similarity. SAAS SAAS023080 HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity. SAAS SAAS023080 HAMAP MF_01202
Sequence similarities	Belongs to the DadA oxidoreductase family. HAMAP MF_01202

Ontologies

Keywords
Ligand	FAD SAAS SAAS023080 HAMAP MF_01202 Flavoprotein SAAS SAAS023080 HAMAP MF_01202
Molecular function	Oxidoreductase SAAS SAAS023080 HAMAP MF_01202 EMBL ACT29459.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C6EGS8 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: EE747358845B6280	FASTA	432	47,607
	10 20 30 40 50 60 MRVVILGSGV VGVASAWYLN QAGHEVTVID REPGAALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAVRLD GTQFQLKWMW QMLRNCDTSH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRAETNIQY EGRQGGTLQL FRTEQQYENA TRDIAVLEDA GVPYQLLESS RLAEVEPALA 190 200 210 220 230 240 EVAHKLTGGL QLPNDETGDC QLFTQNLARM AEQAGVKFRF NTPVDQLLCD GEQIYGVKCG 250 260 270 280 290 300 DEVIKADAYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP IAQEDGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDNRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLYP RGGHVEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTRF KNLWLNTGHG TLGWTMACGS GQLLSDLLSG RTPAIPYEDL SVARYSRGFT 430 PSRPGHLHGA HS

References

[1]	"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed: 19786035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Korea].
[2]	"Sequencing and gene expression analysis of Escherichia coli BL21." Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Austria].
[3]	"Complete sequence of Escherichia coli BL21(DE3)." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [JGI].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001665 Genomic DNA. Translation: ACT29459.1. CP001509 Genomic DNA. Translation: ACT43056.1. AM946981 Genomic DNA. Translation: CAQ31691.1.
3D structure databases
ProteinModelPortal	C6EGS8.
SMR	C6EGS8. Positions 2-34, 200-257.
ModBase	Search...
Protein-protein interaction databases
STRING	C6EGS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000158842; EBESCP00000147170; EBESCG00000156687. EBESCT00000189756; EBESCP00000179109; EBESCG00000189008. EBESCT00000191709; EBESCP00000180470; EBESCG00000192951.
GenomeReviews	Gene locus B21_01174 in contig AM946981_GR. Gene locus ECD_01164 in contig CP001509_GR. Gene locus ECBD_2433 in contig CP001665_GR.
KEGG	ebd:ECBD_2433.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011432.
OMA	VDRQPAV.
ProtClustDB	PRK00711.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6EGS8_ECOBD

Accession

Primary (citable) accession number: C6EGS8
Secondary accession number(s): C5W469

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)