C6EAG9 (C6EAG9_ECOBD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cytidine deaminase PIRNR PIRNR006334 HAMAP MF_01558
EC=3.5.4.5 PIRNR PIRNR006334 HAMAP MF_01558

Alternative name(s):
Cytidine aminohydrolase HAMAP MF_01558

Gene names

Name:	cdd HAMAP MF_01558 EMBL ACT43896.1
Ordered Locus Names:	B21_02031, ECBD_1515, ECD_02072

Organism

Escherichia coli (strain B / BL21-DE3) [Complete proteome] [HAMAP]

Taxonomic identifier

469008 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis By similarity. SAAS SAAS002125 HAMAP MF_01558
Catalytic activity	Cytidine + H2O = uridine + NH3. SAAS SAAS002125 PIRNR PIRNR006334 HAMAP MF_01558
Cofactor	Binds 1 zinc ion By similarity. SAAS SAAS002125 PIRNR PIRNR006334 PIRSR PIRSR006334-3 HAMAP MF_01558
Subunit structure	Homodimer By similarity. SAAS SAAS002125 HAMAP MF_01558
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family. PIRNR PIRNR006334 HAMAP MF_01558

Ontologies

Keywords
Ligand	Metal-binding SAAS SAAS020797 PIRNR PIRNR006334 PIRSR PIRSR006334-3 HAMAP MF_01558 Zinc SAAS SAAS020797 PIRNR PIRNR006334 PIRSR PIRSR006334-3 HAMAP MF_01558
Molecular function	Hydrolase SAAS SAAS002125 PIRNR PIRNR006334 HAMAP MF_01558 EMBL ACT28576.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	cytidine deaminase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	89 – 91	3	Substrate binding By similarity PIRSR PIRSR006334-2
Sites
	Active site	104	1	Proton donor By similarity PIRSR PIRSR006334-1
	Metal binding	102	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3
	Metal binding	129	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3
	Metal binding	132	1	Zinc; catalytic By similarity PIRSR PIRSR006334-3

Sequences

	Sequence		Length	Mass (Da)
	C6EAG9 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: F0B5CD68AB145D7D	FASTA	294	31,540
	10 20 30 40 50 60 MHPRFQTAFA QLADNLQSAL EPILADKYFP ALLTGEQVSS LKSATGLDED ALAFALLPLA 70 80 90 100 110 120 AACARTPLSN FNVGAIARGV SGTWYFGANM EFIGATMQQT VHAEQSAISH AWLSGEKALA 130 140 150 160 170 180 AITVNYTPCG HCRQFMNELN SGLDLRIHLP GREAHALRDY LPDAFGPKDL EIKTLLMDEQ 190 200 210 220 230 240 DHGYALTGDA LSQAAIAAAN RSHMPYSKSP SGVALECKDG RIFSGSYAEN AAFNPTLPPL 250 260 270 280 290 QGALILLNLK GYDYPDIQRA VLAEKADAPL IQWDATSATL KALGCHSIDR VLLA

References

[1]	"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)." Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F. J. Mol. Biol. 394:644-652(2009) [PubMed: 19786035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Korea].
[2]	"Sequencing and gene expression analysis of Escherichia coli BL21." Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [Austria].
[3]	"Complete sequence of Escherichia coli BL21(DE3)." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B / BL21-DE3 [JGI].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001665 Genomic DNA. Translation: ACT28576.1. CP001509 Genomic DNA. Translation: ACT43896.1. AM946981 Genomic DNA. Translation: CAQ32548.1.
3D structure databases
ProteinModelPortal	C6EAG9.
SMR	C6EAG9. Positions 1-294.
ModBase	Search...
Protein-protein interaction databases
STRING	C6EAG9.
Proteomic databases
PRIDE	C6EAG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000156839; EBESCP00000146993; EBESCG00000155295. EBESCT00000189578; EBESCP00000178941; EBESCG00000189030. EBESCT00000195833; EBESCP00000182992; EBESCG00000192539.
GenomeReviews	Gene locus B21_02031 in contig AM946981_GR. Gene locus ECD_02072 in contig CP001509_GR. Gene locus ECBD_1515 in contig CP001665_GR.
KEGG	ebd:ECBD_1515.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010652.
OMA	NRSHAPY.
ProtClustDB	PRK09027.
Family and domain databases
HAMAP	MF_01558. Cyt_deam. [Tree]
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR002125. CMP_dCMP_Zn-bd. IPR013171. Cyd/dCyd_deaminase_Zn-bd. IPR006263. Cyt_deam_dimer. IPR016193. Cytidine_deaminase-like. IPR020797. Cytidine_deaminase_bacteria. [Graphical view]
KO	K01489.
PANTHER	PTHR11644:SF12. PTHR11644:SF12. 1 hit.
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. PF08211. dCMP_cyt_deam_2. 1 hit. [Graphical view]
PIRSF	PIRSF006334. Cdd_plus_pseudo. 1 hit.
SUPFAM	SSF53927. Cytidine_deaminase-like. 2 hits.
TIGRFAMs	TIGR01355. Cyt_deam_dimer. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6EAG9_ECOBD

Accession

Primary (citable) accession number: C6EAG9
Secondary accession number(s): C5W6N9

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)