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C6E9S6

- C6E9S6_ECOBD

UniProt

C6E9S6 - C6E9S6_ECOBD

Protein

NADH-quinone oxidoreductase subunit N

Gene

nuoN

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    TransportUniRule annotation

    Keywords - Ligandi

    NADUniRule annotation, UbiquinoneUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit NUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit NUniRule annotation
    NDH-1 subunit NUniRule annotation
    Gene namesi
    Name:nuoNUniRule annotation
    Ordered Locus Names:ECBD_1385Imported
    OrganismiEscherichia coli (strain B / BL21-DE3)Imported
    Taxonomic identifieri469008 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000002032: Chromosome

    Subcellular locationi

    Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
    Membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membraneUniRule annotation, Cell membrane, Membrane

    PTM / Processingi

    Keywords - PTMi

    QuinoneUniRule annotation

    Interactioni

    Subunit structurei

    NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-59174N.
    STRINGi469008.ECBD_1385.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RKOX-ray3.00D/N1-485[»]
    ProteinModelPortaliC6E9S6.
    SMRiC6E9S6. Positions 1-485.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical;UniRule annotationAdd
    BLAST
    Transmembranei35 – 5521Helical;UniRule annotationAdd
    BLAST
    Transmembranei71 – 9121Helical;UniRule annotationAdd
    BLAST
    Transmembranei105 – 12521Helical;UniRule annotationAdd
    BLAST
    Transmembranei127 – 14721Helical;UniRule annotationAdd
    BLAST
    Transmembranei159 – 17921Helical;UniRule annotationAdd
    BLAST
    Transmembranei203 – 22321Helical;UniRule annotationAdd
    BLAST
    Transmembranei235 – 25521Helical;UniRule annotationAdd
    BLAST
    Transmembranei271 – 29121Helical;UniRule annotationAdd
    BLAST
    Transmembranei297 – 31721Helical;UniRule annotationAdd
    BLAST
    Transmembranei326 – 34621Helical;UniRule annotationAdd
    BLAST
    Transmembranei373 – 39321Helical;UniRule annotationAdd
    BLAST
    Transmembranei408 – 43023Helical;UniRule annotationAdd
    BLAST
    Transmembranei455 – 47521Helical;UniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 2 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helixUniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000100796.
    KOiK00343.
    OMAiSIVQLAM.

    Family and domain databases

    HAMAPiMF_00445. NDH1_NuoN_1.
    InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    [Graphical view]
    PfamiPF00361. Oxidored_q1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C6E9S6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTITPQNLIA LLPLLIVGLT VVVVMLSIAW RRNHFLNATL SVIGLNAALV    50
    SLWFVGQAGA MDVTPLMRVD GFAMLYTGLV LLASLATCTF AYPWLEGYND 100
    NKDEFYLLVL IAALGGILLA NANHLASLFL GIELISLPLF GLVGYAFRQK 150
    RSLEASIKYT ILSAAASSFL LFGMALVYAQ SGDLSFVALG KNLGDGMLNE 200
    PLLLAGFGLM IVGLGFKLSL VPFHLWTPDV YQGAPAPVST FLATASKIAI 250
    FGVVMRLFLY APVGDSEAIR VVLAIIAFAS IIFGNLMALS QTNIKRLLGY 300
    SSISHLGYLL VALIALQTGE MSMEAVGVYL AGYLFSSLGA FGVVSLMSSP 350
    YRGPDADSLF SYRGLFWHRP ILAAVMTVMM LSLAGIPMTL GFIGKFYVLA 400
    VGVQAHLWWL VGAVVVGSAI GLYYYLRVAV SLYLHAPEQP GRDAPSNWQY 450
    SAGGIVVLIS ALLVLVLGVW PQPLISIVRL AMPLM 485
    Length:485
    Mass (Da):52,044
    Last modified:September 1, 2009 - v1
    Checksum:iA15F4875B7D19D09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001665 Genomic DNA. Translation: ACT28448.1.
    RefSeqiYP_003035633.1. NC_012947.1.

    Genome annotation databases

    EnsemblBacteriaiACT28448; ACT28448; ECBD_1385.
    GeneIDi8156289.
    KEGGiebd:ECBD_1385.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001665 Genomic DNA. Translation: ACT28448.1 .
    RefSeqi YP_003035633.1. NC_012947.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RKO X-ray 3.00 D/N 1-485 [» ]
    ProteinModelPortali C6E9S6.
    SMRi C6E9S6. Positions 1-485.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59174N.
    STRINGi 469008.ECBD_1385.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT28448 ; ACT28448 ; ECBD_1385 .
    GeneIDi 8156289.
    KEGGi ebd:ECBD_1385.

    Phylogenomic databases

    HOGENOMi HOG000100796.
    KOi K00343.
    OMAi SIVQLAM.

    Family and domain databases

    HAMAPi MF_00445. NDH1_NuoN_1.
    InterProi IPR010096. NADH-Q_OxRdtase_suN/2.
    IPR001750. NADH_UbQ/plastoQ_OxRdtase.
    [Graphical view ]
    Pfami PF00361. Oxidored_q1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01770. NDH_I_N. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Escherichia coli BL21(DE3)."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B / BL21-DE3 [JGI]Imported.
    2. "Structure of the membrane domain of respiratory complex I."
      Efremov R.G., Sazanov L.A.
      Nature 476:414-420(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

    Entry informationi

    Entry nameiC6E9S6_ECOBD
    AccessioniPrimary (citable) accession number: C6E9S6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3