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C6E9S6

- C6E9S6_ECOBD

UniProt

C6E9S6 - C6E9S6_ECOBD

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Protein

NADH-quinone oxidoreductase subunit N

Gene

nuoN

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

TransportUniRule annotation

Keywords - Ligandi

NADUniRule annotation, UbiquinoneUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit NUniRule annotation (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit NUniRule annotation
NDH-1 subunit NUniRule annotation
Gene namesi
Name:nuoNUniRule annotation
Ordered Locus Names:ECBD_1385Imported
OrganismiEscherichia coli (strain B / BL21-DE3)Imported
Taxonomic identifieri469008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000002032: Chromosome

Subcellular locationi

Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalUniRule annotationAdd
BLAST
Transmembranei35 – 5521HelicalUniRule annotationAdd
BLAST
Transmembranei71 – 9121HelicalUniRule annotationAdd
BLAST
Transmembranei105 – 12521HelicalUniRule annotationAdd
BLAST
Transmembranei127 – 14721HelicalUniRule annotationAdd
BLAST
Transmembranei159 – 17921HelicalUniRule annotationAdd
BLAST
Transmembranei203 – 22321HelicalUniRule annotationAdd
BLAST
Transmembranei235 – 25521HelicalUniRule annotationAdd
BLAST
Transmembranei271 – 29121HelicalUniRule annotationAdd
BLAST
Transmembranei297 – 31721HelicalUniRule annotationAdd
BLAST
Transmembranei326 – 34621HelicalUniRule annotationAdd
BLAST
Transmembranei373 – 39321HelicalUniRule annotationAdd
BLAST
Transmembranei408 – 43023HelicalUniRule annotationAdd
BLAST
Transmembranei455 – 47521HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

PTM / Processingi

Keywords - PTMi

QuinoneUniRule annotation

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59174N.
STRINGi469008.ECBD_1385.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKOX-ray3.00D/N1-485[»]
ProteinModelPortaliC6E9S6.
SMRiC6E9S6. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

HOGENOMiHOG000100796.
KOiK00343.
OMAiSIVQLAM.

Family and domain databases

HAMAPiMF_00445. NDH1_NuoN_1.
InterProiIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamiPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01770. NDH_I_N. 1 hit.

Sequencei

Sequence statusi: Complete.

C6E9S6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTITPQNLIA LLPLLIVGLT VVVVMLSIAW RRNHFLNATL SVIGLNAALV
60 70 80 90 100
SLWFVGQAGA MDVTPLMRVD GFAMLYTGLV LLASLATCTF AYPWLEGYND
110 120 130 140 150
NKDEFYLLVL IAALGGILLA NANHLASLFL GIELISLPLF GLVGYAFRQK
160 170 180 190 200
RSLEASIKYT ILSAAASSFL LFGMALVYAQ SGDLSFVALG KNLGDGMLNE
210 220 230 240 250
PLLLAGFGLM IVGLGFKLSL VPFHLWTPDV YQGAPAPVST FLATASKIAI
260 270 280 290 300
FGVVMRLFLY APVGDSEAIR VVLAIIAFAS IIFGNLMALS QTNIKRLLGY
310 320 330 340 350
SSISHLGYLL VALIALQTGE MSMEAVGVYL AGYLFSSLGA FGVVSLMSSP
360 370 380 390 400
YRGPDADSLF SYRGLFWHRP ILAAVMTVMM LSLAGIPMTL GFIGKFYVLA
410 420 430 440 450
VGVQAHLWWL VGAVVVGSAI GLYYYLRVAV SLYLHAPEQP GRDAPSNWQY
460 470 480
SAGGIVVLIS ALLVLVLGVW PQPLISIVRL AMPLM
Length:485
Mass (Da):52,044
Last modified:September 1, 2009 - v1
Checksum:iA15F4875B7D19D09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001665 Genomic DNA. Translation: ACT28448.1.
RefSeqiYP_003035633.1. NC_012947.1.

Genome annotation databases

EnsemblBacteriaiACT28448; ACT28448; ECBD_1385.
GeneIDi8156289.
KEGGiebd:ECBD_1385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001665 Genomic DNA. Translation: ACT28448.1 .
RefSeqi YP_003035633.1. NC_012947.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RKO X-ray 3.00 D/N 1-485 [» ]
ProteinModelPortali C6E9S6.
SMRi C6E9S6. Positions 1-485.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59174N.
STRINGi 469008.ECBD_1385.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT28448 ; ACT28448 ; ECBD_1385 .
GeneIDi 8156289.
KEGGi ebd:ECBD_1385.

Phylogenomic databases

HOGENOMi HOG000100796.
KOi K00343.
OMAi SIVQLAM.

Family and domain databases

HAMAPi MF_00445. NDH1_NuoN_1.
InterProi IPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view ]
Pfami PF00361. Oxidored_q1. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01770. NDH_I_N. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Escherichia coli BL21(DE3)."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B / BL21-DE3 [JGI]Imported.
  2. "Structure of the membrane domain of respiratory complex I."
    Efremov R.G., Sazanov L.A.
    Nature 476:414-420(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

Entry informationi

Entry nameiC6E9S6_ECOBD
AccessioniPrimary (citable) accession number: C6E9S6
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteomeImported

External Data

Dasty 3