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C6E9R4

- C6E9R4_ECOBD

UniProt

C6E9R4 - C6E9R4_ECOBD

Protein

NADH-quinone oxidoreductase subunit A

Gene

nuoA

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain.UniRule annotation
    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Biological processi

    TransportUniRule annotation

    Keywords - Ligandi

    NADUniRule annotation, UbiquinoneUniRule annotationImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit AUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit AUniRule annotation
    NDH-1 subunit AUniRule annotation
    NUO1UniRule annotation
    Gene namesi
    Name:nuoAUniRule annotationImported
    Ordered Locus Names:B21_02173Imported, ECBD_1373Imported, ECD_02213Imported
    OrganismiEscherichia coli (strain B / BL21-DE3)Imported
    Taxonomic identifieri469008 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000009074: Chromosome, UP000002032: Chromosome, UP000001509: Chromosome

    Subcellular locationi

    Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
    Membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membraneUniRule annotation, Cell membrane, Membrane

    PTM / Processingi

    Keywords - PTMi

    QuinoneUniRule annotation

    Interactioni

    Subunit structurei

    NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-59171N.
    STRINGi469008.ECBD_1373.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RKOX-ray3.00A/E1-147[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621Helical;UniRule annotationAdd
    BLAST
    Transmembranei68 – 8821Helical;UniRule annotationAdd
    BLAST
    Transmembranei98 – 11821Helical;UniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 3 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helixUniRule annotation

    Phylogenomic databases

    eggNOGiCOG0838.
    HOGENOMiHOG000100121.
    KOiK00330.
    OMAiWVGFIEA.

    Family and domain databases

    HAMAPiMF_01394. NDH1_NuoA.
    InterProiIPR023043. NAD(P)H_OxRDtase_bac/plastid.
    IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
    [Graphical view]
    PANTHERiPTHR11058. PTHR11058. 1 hit.
    PfamiPF00507. Oxidored_q4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6E9R4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF    50
    ESGIDSVGSA RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF 100
    VEAAIFIFVL LAGLVYLVRI GALDWTPARS RRERMNPETN SIANRQR 147
    Length:147
    Mass (Da):16,457
    Last modified:September 1, 2009 - v1
    Checksum:i1F9E0804B2B0D1B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001665 Genomic DNA. Translation: ACT28436.1.
    CP001509 Genomic DNA. Translation: ACT44035.1.
    AM946981 Genomic DNA. Translation: CAQ32690.1.
    RefSeqiYP_002999952.1. NC_012892.2.
    YP_003035621.1. NC_012947.1.
    YP_003054806.1. NC_012971.2.

    Genome annotation databases

    EnsemblBacteriaiACT28436; ACT28436; ECBD_1373.
    ACT44035; ACT44035; ECD_02213.
    CAQ32690; CAQ32690; B21_02173.
    GeneIDi8113024.
    8156277.
    8181263.
    KEGGiebd:ECBD_1373.
    ebe:B21_02173.
    ebl:ECD_02213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001665 Genomic DNA. Translation: ACT28436.1 .
    CP001509 Genomic DNA. Translation: ACT44035.1 .
    AM946981 Genomic DNA. Translation: CAQ32690.1 .
    RefSeqi YP_002999952.1. NC_012892.2.
    YP_003035621.1. NC_012947.1.
    YP_003054806.1. NC_012971.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RKO X-ray 3.00 A/E 1-147 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59171N.
    STRINGi 469008.ECBD_1373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT28436 ; ACT28436 ; ECBD_1373 .
    ACT44035 ; ACT44035 ; ECD_02213 .
    CAQ32690 ; CAQ32690 ; B21_02173 .
    GeneIDi 8113024.
    8156277.
    8181263.
    KEGGi ebd:ECBD_1373.
    ebe:B21_02173.
    ebl:ECD_02213.

    Phylogenomic databases

    eggNOGi COG0838.
    HOGENOMi HOG000100121.
    KOi K00330.
    OMAi WVGFIEA.

    Family and domain databases

    HAMAPi MF_01394. NDH1_NuoA.
    InterProi IPR023043. NAD(P)H_OxRDtase_bac/plastid.
    IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
    [Graphical view ]
    PANTHERi PTHR11058. PTHR11058. 1 hit.
    Pfami PF00507. Oxidored_q4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and gene expression analysis of Escherichia coli BL21(DE3)."
      Krempl P.M., Mairhofer J., Eisenkolb M., Specht T., Leparc G.G., Kreil D.P., Bayer K., Striedner G.
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BL21Imported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B / BL21-DE3 [Austria]Imported, B / BL21-DE3 [JGI]Imported, B / BL21-DE3 [Korea]Imported and BL21Imported.
    3. "Sequencing and gene expression analysis of Escherichia coli BL21."
      Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.
      Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Complete sequence of Escherichia coli 'BL21-Gold(DE3)pLysS AG'."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BL21-GoldImported.
    5. "Complete sequence of Escherichia coli BL21(DE3)."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Jeong H., Shim J.-H., Studier F.W., Oh T.K., Kim J.F.
      Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BL21.
    7. "Structure of the membrane domain of respiratory complex I."
      Efremov R.G., Sazanov L.A.
      Nature 476:414-420(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

    Entry informationi

    Entry nameiC6E9R4_ECOBD
    AccessioniPrimary (citable) accession number: C6E9R4
    Secondary accession number(s): C5W725
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3