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C6E9R4 (C6E9R4_ECOBD) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit A HAMAP-Rule MF_01394

EC=1.6.99.5 HAMAP-Rule MF_01394
Alternative name(s):
NADH dehydrogenase I subunit A HAMAP-Rule MF_01394
NDH-1 subunit A HAMAP-Rule MF_01394
NUO1 HAMAP-Rule MF_01394
Gene names
Name:nuoA HAMAP-Rule MF_01394 EMBL ACT44035.1
Ordered Locus Names:B21_02173 EMBL CAQ32690.1, ECBD_1373 EMBL ACT28436.1, ECD_02213 EMBL ACT44035.1
OrganismEscherichia coli (strain B / BL21-DE3) [Complete proteome] [HAMAP]
Taxonomic identifier469008 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain By similarity. RuleBase RU003639

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01394

Catalytic activity

NADH + quinone = NAD+ + quinol. RuleBase RU003639 HAMAP-Rule MF_01394

Subunit structure

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity. HAMAP-Rule MF_01394

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01394.

Membrane; Multi-pass membrane protein By similarity RuleBase RU003639.

Sequence similarities

Belongs to the complex I subunit 3 family. RuleBase RU003639 HAMAP-Rule MF_01394

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Transmembrane16 – 3621Helical; By similarity HAMAP-Rule MF_01394
Transmembrane68 – 8821Helical; By similarity HAMAP-Rule MF_01394
Transmembrane98 – 11821Helical; By similarity HAMAP-Rule MF_01394

Sequences

Sequence LengthMass (Da)Tools
C6E9R4 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: 1F9E0804B2B0D1B5

FASTA14716,457
        10         20         30         40         50         60 
MSMSTSTEVI AHHWAFAIFL IVAIGLCCLM LVGGWFLGGR ARARSKNVPF ESGIDSVGSA 

        70         80         90        100        110        120 
RLRLSAKFYL VAMFFVIFDV EALYLFAWST SIRESGWVGF VEAAIFIFVL LAGLVYLVRI 

       130        140 
GALDWTPARS RRERMNPETN SIANRQR 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and gene expression analysis of Escherichia coli BL21(DE3)."
Krempl P.M., Mairhofer J., Eisenkolb M., Specht T., Leparc G.G., Kreil D.P., Bayer K., Striedner G.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BL21 EMBL CAQ32690.1.
[2]"Genome sequences of Escherichia coli B strains REL606 and BL21(DE3)."
Jeong H., Barbe V., Lee C.H., Vallenet D., Yu D.S., Choi S.H., Couloux A., Lee S.W., Yoon S.H., Cattolico L., Hur C.G., Park H.S., Segurens B., Kim S.C., Oh T.K., Lenski R.E., Studier F.W., Daegelen P., Kim J.F.
J. Mol. Biol. 394:644-652(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [Korea] and BL21 EMBL ACT44035.1.
[3]"Sequencing and gene expression analysis of Escherichia coli BL21."
Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [Austria].
[4]"Complete sequence of Escherichia coli 'BL21-Gold(DE3)pLysS AG'."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BL21-Gold EMBL ACT28436.1.
[5]"Complete sequence of Escherichia coli BL21(DE3)."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B / BL21-DE3 [JGI].
[6]Jeong H., Shim J.-H., Studier F.W., Oh T.K., Kim J.F.
Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BL21.
[7]"Structure of the membrane domain of respiratory complex I."
Efremov R.G., Sazanov L.A.
Nature 476:414-420(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001665 Genomic DNA. Translation: ACT28436.1.
CP001509 Genomic DNA. Translation: ACT44035.1.
AM946981 Genomic DNA. Translation: CAQ32690.1.
RefSeqYP_002999952.1. NC_012892.2.
YP_003035621.1. NC_012947.1.
YP_003054806.1. NC_012971.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKOX-ray3.00A/E1-147[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59171N.
STRING469008.ECBD_1373.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACT28436; ACT28436; ECBD_1373.
ACT44035; ACT44035; ECD_02213.
CAQ32690; CAQ32690; B21_02173.
GeneID8113024.
8156277.
8181263.
KEGGebd:ECBD_1373.
ebe:B21_02173.
ebl:ECD_02213.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0838.
HOGENOMHOG000100121.
KOK00330.
OMAWVGFIEA.

Family and domain databases

HAMAPMF_01394. NDH1_NuoA.
InterProIPR023043. NAD(P)H_OxRDtase_bac/plastid.
IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3.
[Graphical view]
PANTHERPTHR11058. PTHR11058. 1 hit.
PfamPF00507. Oxidored_q4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC6E9R4_ECOBD
AccessionPrimary (citable) accession number: C6E9R4
Secondary accession number(s): C5W725
Entry history
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)