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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacter sp. (strain M21)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), 6-phosphofructokinase (GM21_1392), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP; via amide nitrogenUniRule annotation1
Metal bindingi116Magnesium; catalyticUniRule annotation1
Sitei117Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei140Proton acceptorUniRule annotation1
Binding sitei175Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei235SubstrateUniRule annotation1
Binding sitei279Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 76ATPUniRule annotation2
Nucleotide bindingi115 – 118ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSP443144:GHKM-1237-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:GM21_1236Imported
OrganismiGeobacter sp. (strain M21)Imported
Taxonomic identifieri443144 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000002380 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi443144.GM21_1236.

Structurei

3D structure databases

ProteinModelPortaliC6E3N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 311PFKInterPro annotationAdd BLAST308

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 140Substrate bindingUniRule annotation3
Regioni182 – 184Substrate bindingUniRule annotation3
Regioni285 – 288Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiIELMGHK.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6E3N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKIGILTG GGDCPGLNAV IRGVVKSATG RGWEVVGIED GFDGFLHPEK
60 70 80 90 100
CRCLGLEEVR GILPLGGTIL GTSNRGNPFA FPIVREGKVE LVDVSDEVCS
110 120 130 140 150
RIEHLGLSAL IVVGGDGSLK IALEMMRRGV PIVGVPKTID NDLLETDVTF
160 170 180 190 200
GYNTALETAT EALDKLHSTA ESHHRVMVLE VMGRYAGWIA LESGIAGGAD
210 220 230 240 250
VILIPEIPYD IDKVSEAILA RGRRGRRFSV VVVAEGAFPK GGGRVVKEAA
260 270 280 290 300
GGKSVVERLG GVGEAFSREL GGVLEMDIRV TVLGHLQRGG SPSTFDRCIG
310 320 330 340 350
SRFGVKAVEL IESGAFGHMA CLKGRHVTSV PIEDAVRSLN LINPDGEMVR
360
HAEALGIMLG R
Length:361
Mass (Da):38,132
Last modified:September 1, 2009 - v1
Checksum:i11AA3FFD91369527
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001661 Genomic DNA. Translation: ACT17297.1.
RefSeqiWP_015836549.1. NC_012918.1.

Genome annotation databases

EnsemblBacteriaiACT17297; ACT17297; GM21_1236.
KEGGigem:GM21_1236.
PATRICi22016930. VBIGeoSp56140_1202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001661 Genomic DNA. Translation: ACT17297.1.
RefSeqiWP_015836549.1. NC_012918.1.

3D structure databases

ProteinModelPortaliC6E3N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi443144.GM21_1236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT17297; ACT17297; GM21_1236.
KEGGigem:GM21_1236.
PATRICi22016930. VBIGeoSp56140_1202.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiIELMGHK.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciGSP443144:GHKM-1237-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC6E3N2_GEOSM
AccessioniPrimary (citable) accession number: C6E3N2
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.