C6E2P5 (PNP_GEOSM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8

Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	GM21_2976

Organism

Geobacter sp. (strain M21) [Complete proteome] [HAMAP]

Taxonomic identifier

443144 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: HAMAP polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 696	696	Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595		PRO_1000215660
Regions
	Domain	550 – 609	60	KH
	Domain	619 – 687	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	C6E2P5 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: CC647B2B27D41FF7	FASTA	696	74,536
	10 20 30 40 50 60 MVHTVQAECC GKTITIETGK IAKQASGAVM IKSGDTMVLV TAVAMKSAKE GQGFFPLTVN 70 80 90 100 110 120 YQEKAYAGGR IPGSFFKREG RPSDNETLTC RLIDRPIRPL FPEGFLNDTQ VMATVVSADK 130 140 150 160 170 180 DNDPAILSMI GASAALMVSD CPFAGPIAGV KVGRVNGAFI ANPTAEELEK SDLEIVIAAS 190 200 210 220 230 240 QEAILMVEGS AAEVSENDLL EAIFFGHAAV QPILAAQLEL AKKVGTPKRV ILAPVVNEEL 250 260 270 280 290 300 KARVAALAKE GMKQAVRIKT KVERHLAIDA ITAETVAALA AEFEGSEKEV KGFIEDLEYD 310 320 330 340 350 360 LVREHIIKDG QRIDGRDTKT IRAISTEVSL LPRAHGSALF TRGETQSIVA ATLGTSVDEQ 370 380 390 400 410 420 RIDSLYGDSR KKFMLHYNFP PYSVGETSFR LAPGRREIGH GMLAERALQQ VLPKHDDFPY 430 440 450 460 470 480 TIRIVSDITE SNGSSSMATV CGGSLSMMDA GIPIKAPVAG IAMGLIKEGD DFAILSDILG 490 500 510 520 530 540 DEDHLGDMDF KVAGTAEGVT ALQMDIKIGG VTREIMSAAL AQAKAGRIHI LGEMAKTIAA 550 560 570 580 590 600 SRGDLSAFAP RITTIWVKVD KIRDVIGSGG KNIRSVTEAT GVSIDIDDTG KINIASTNKE 610 620 630 640 650 660 ACDLAIKMIR NLTAEAEEGK LYMGTVKKIM EFGAFVEIFP GTDGLVHVSE LDTERVKNVS 670 680 690 DILKEGDKVL VKCIGIDKQG KIKLSRKEAL GQTFTE

References

[1]

"Complete sequence of Geobacter sp. M21." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lovley D. Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: M21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001661 Genomic DNA. Translation: ACT19005.1.
RefSeq	YP_003022763.1. NC_012918.1.
3D structure databases
ProteinModelPortal	C6E2P5.
ModBase	Search...
Protein-protein interaction databases
STRING	443144.GM21_2976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACT19005; ACT19005; GM21_2976.
GeneID	8138319.
KEGG	gem:GM21_2976.
PATRIC	22020324. VBIGeoSp56140_2883.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1185.
HOGENOM	HOG000218327.
KO	K00962.
OMA	YTMRVVS.
ProtClustDB	PRK11824.
Enzyme and pathway databases
BioCyc	GSP443144:GHKM-3020-MONOMER.
Family and domain databases
Gene3D	1.10.10.400. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_01595. PNPase.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR009019. KH_prok-type. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR022967. RNA-binding_domain_S1. [Graphical view]
PANTHER	PTHR11252. PTHR11252. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF46915. 3_ExoRNase. 1 hit. SSF55666. 3_ExoRNase. 2 hits. SSF54814. KH_prok. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR03591. polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_GEOSM

Accession

Primary (citable) accession number: C6E2P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program