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C6E100 (SYL_GEOSM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:GM21_0766
OrganismGeobacter sp. (strain M21) [Complete proteome] [HAMAP]
Taxonomic identifier443144 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202220

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C6E100 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: 873509B070F041FC

FASTA82492,698
        10         20         30         40         50         60 
MEEKYNPSAV EEKWQGYWAE HESFKATEDP TRKKYYLLEM FPYPSGKIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VIARFKRMQG YNVLHPMGWD AFGMPAENAA IQHKSHPAKW TYENIAYMRG QLKTLGLSYD 

       130        140        150        160        170        180 
WDRELATCDL DYYKWEQRIF LEMYKKGLAY KKSSAVNWCP KCETVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
SPVRQKELEQ WSFRITNYAQ ELLDDTYKLT GWPERVLTMQ RNWIGRSTGC EIDFPLENGL 

       250        260        270        280        290        300 
GKIKVFTTRQ DTLFGATFMS LAAEHPMALD LAGDAQRAQV EAFIDKVKKT DRIKRGAEDL 

       310        320        330        340        350        360 
EKEGVFTGSY CVNPVTNTKM PIYLANFVLM DYGTGAVMAV PTHDQRDFEF AKKYNLPLKV 

       370        380        390        400        410        420 
VIQPEGETLD PAAMTEAYTA EGIMANSGRF DGMGNGDAKE AIADFLEKEG IGKKTVNFRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPVINC DLCGVVAVPE ADLPVVLPMD AEFTGEGGNP LARVDSFTTC 

       490        500        510        520        530        540 
TCPQCGEAAR RETDTMDTFV QSSWYFLRYC SPKFSAGPLD REKVEAWMPV DQYIGGIEHA 

       550        560        570        580        590        600 
VLHLLYARFF TKVLRDLGYC NVDEPFSNLL TQGMVIKDGA KMSKSKGNVV DPNALIERYG 

       610        620        630        640        650        660 
ADTARLFSLF AAPPEKDLDW SDQGVDGSYR FLNRVWRLVY DVLPVIGEAG AVNPDSLGAE 

       670        680        690        700        710        720 
AKKLRRAVHK TIKKVSEDVE ERFHFNTAIA AVMELVNAIQ AFAAKDAPEN VAVVREAVES 

       730        740        750        760        770        780 
VVRLLAPFVP HFAEELWSQL GHDTVLEAAG WPGYDAAAVV DEEVTVVIQV NGKLRSKLTV 

       790        800        810        820 
APDAKEEEVR AQALADDKIK PYLEGKDVKK VVYVPGKLVS IVVA 

« Hide

References

[1]"Complete sequence of Geobacter sp. M21."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Lovley D.
Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M21.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001661 Genomic DNA. Translation: ACT16840.1.
RefSeqYP_003020598.1. NC_012918.1.

3D structure databases

ProteinModelPortalC6E100.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING443144.GM21_0766.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACT16840; ACT16840; GM21_0766.
GeneID8136081.
KEGGgem:GM21_0766.
PATRIC22016026. VBIGeoSp56140_0760.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycGSP443144:GHKM-782-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEOSM
AccessionPrimary (citable) accession number: C6E100
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries