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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium tuberculosis (strain KZN 1435 / MDR)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 5311D-inositol 3-phosphateUniRule annotation
Binding sitei67 – 671UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei122 – 12211D-inositol 3-phosphateUniRule annotation
Binding sitei155 – 15511D-inositol 3-phosphateUniRule annotation
Binding sitei179 – 17911D-inositol 3-phosphateUniRule annotation
Binding sitei199 – 19911D-inositol 3-phosphateUniRule annotation
Binding sitei273 – 2731UDP-GlcNAcUniRule annotation
Binding sitei278 – 2781UDP-GlcNAcUniRule annotation
Binding sitei331 – 3311UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi340 – 3401Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi341 – 3411Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi343 – 3431Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei353 – 3531UDP-GlcNAcUniRule annotation
Binding sitei361 – 3611UDP-GlcNAcUniRule annotation
Metal bindingi367 – 3671MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTUB478434:GH8E-503-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:TBMG_00489
OrganismiMycobacterium tuberculosis (strain KZN 1435 / MDR)
Taxonomic identifieri478434 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480D-inositol 3-phosphate glycosyltransferasePRO_0000400143Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC6DT68.
SMRiC6DT68. Positions 46-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 602UDP-GlcNAc bindingUniRule annotation
Regioni64 – 6961D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

C6DT68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVRHDDGS GLIAQRRPVR GEGATRSRGP SGPSNRNVSA ADDPRRVALL
60 70 80 90 100
AVHTSPLAQP GTGDAGGMNV YMLQSALHLA RRGIEVEIFT RATASADPPV
110 120 130 140 150
VRVAPGVLVR NVVAGPFEGL DKYDLPTQLC AFAAGVLRAE AVHEPGYYDI
160 170 180 190 200
VHSHYWLSGQ VGWLARDRWA VPLVHTAHTL AAVKNAALAD GDGPEPPLRT
210 220 230 240 250
VGEQQVVDEA DRLIVNTDDE ARQVISLHGA DPARIDVVHP GVDLDVFRPG
260 270 280 290 300
DRRAARAALG LPVDERVVAF VGRIQPLKAP DIVLRAAAKL PGVRIIVAGG
310 320 330 340 350
PSGSGLASPD GLVRLADELG ISARVTFLPP QSHTDLATLF RAADLVAVPS
360 370 380 390 400
YSESFGLVAV EAQACGTPVV AAAVGGLPVA VRDGITGTLV SGHEVGQWAD
410 420 430 440 450
AIDHLLRLCA GPRGRVMSRA AARHAATFSW ENTTDALLAS YRRAIGEYNA
460 470 480
ERQRRGGEVI SDLVAVGKPR HWTPRRGVGA
Length:480
Mass (Da):50,541
Last modified:September 1, 2009 - v1
Checksum:i2134755E894A9CCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001658 Genomic DNA. Translation: ACT23520.1.

Genome annotation databases

EnsemblBacteriaiACT23520; ACT23520; TBMG_00489.
KEGGimtb:TBMG_00489.
PATRICi18158411. VBIMycTub122270_0531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001658 Genomic DNA. Translation: ACT23520.1.

3D structure databases

ProteinModelPortaliC6DT68.
SMRiC6DT68. Positions 46-446.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT23520; ACT23520; TBMG_00489.
KEGGimtb:TBMG_00489.
PATRICi18158411. VBIMycTub122270_0531.

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Enzyme and pathway databases

BioCyciMTUB478434:GH8E-503-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCTK
AccessioniPrimary (citable) accession number: C6DT68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: September 1, 2009
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.