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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi81Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei83Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei150Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei179Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei372Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei376Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei482Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei531Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei558Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei794Substrate; via amide nitrogenUniRule annotation1
Binding sitei802Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei819Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPCAR561230:G1GF8-2439-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PC1_2411
OrganismiPectobacterium carotovorum subsp. carotovorum (strain PC1)
Taxonomic identifieri561230 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000002736 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_500048644132 – 828Periplasmic nitrate reductaseUniRule annotationAdd BLAST797

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi561230.PC1_2411

Structurei

3D structure databases

ProteinModelPortaliC6DK59
SMRiC6DK59
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 954Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 219Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni243 – 247Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni262 – 264Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni508 – 509Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni718 – 727Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C6DK59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSEAIH WDKAPCRFCG
60 70 80 90 100
VGCGVLVGTQ NGRIVASQGD PDAPVNRGLN CIKGYFLPKI MYGQDRLTQP
110 120 130 140 150
LLRMRDGKFD KEGEFTPISW DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ
160 170 180 190 200
STIWEGYAAA KLFKAGFRSN NIDPNARHCM ASAVVGFMRT FGMDEPMGCY
210 220 230 240 250
DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA VLSTYQHRSF
260 270 280 290 300
ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG
310 320 330 340 350
YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD
360 370 380 390 400
QLEALAKLYA DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT
460 470 480 490 500
GTIPEKIGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NQERMPGWRD
510 520 530 540 550
PRNFIVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK
560 570 580 590 600
APGESKSDLW QVVSFAKRFA VEEVWPEELL AQKPAYRGKT LYDVLFANDV
610 620 630 640 650
TTRFPLSELA ENQLNDESRD FGFYLQKGLF EEYAAFGRGH GHDLAPFDDY
660 670 680 690 700
HKARGLRWPV VNGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP
710 720 730 740 750
YEPAAEAPDD EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP
760 770 780 790 800
QDAKARDLRR GEKVRIISRR GEVVSVVETR GRNKPPRGLV YMPFFDAAQM
810 820
TNVLTLDATD PLSKETDFKK CAVKLAKV
Length:828
Mass (Da):92,674
Last modified:September 1, 2009 - v1
Checksum:iEF943C87CD7EE25B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001657 Genomic DNA Translation: ACT13442.1
RefSeqiWP_015840624.1, NC_012917.1

Genome annotation databases

EnsemblBacteriaiACT13442; ACT13442; PC1_2411
GeneIDi29703804
KEGGipct:PC1_2411

Similar proteinsi

Entry informationi

Entry nameiNAPA_PECCP
AccessioniPrimary (citable) accession number: C6DK59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: March 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health