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C6DK59 (NAPA_PECCP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:PC1_2411
OrganismPectobacterium carotovorum subsp. carotovorum (strain PC1) [Complete proteome] [HAMAP]
Taxonomic identifier561230 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 828797Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000486441

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
C6DK59 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: EF943C87CD7EE25B

FASTA82892,674
        10         20         30         40         50         60 
MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSEAIH WDKAPCRFCG VGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PDAPVNRGLN CIKGYFLPKI MYGQDRLTQP LLRMRDGKFD KEGEFTPISW 

       130        140        150        160        170        180 
DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ STIWEGYAAA KLFKAGFRSN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA 

       250        260        270        280        290        300 
VLSTYQHRSF ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG 

       310        320        330        340        350        360 
YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD QLEALAKLYA 

       370        380        390        400        410        420 
DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT GTIPEKIGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NQERMPGWRD PRNFIVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVK APGESKSDLW QVVSFAKRFA VEEVWPEELL AQKPAYRGKT LYDVLFANDV 

       610        620        630        640        650        660 
TTRFPLSELA ENQLNDESRD FGFYLQKGLF EEYAAFGRGH GHDLAPFDDY HKARGLRWPV 

       670        680        690        700        710        720 
VNGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP YEPAAEAPDD EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP QDAKARDLRR GEKVRIISRR GEVVSVVETR 

       790        800        810        820 
GRNKPPRGLV YMPFFDAAQM TNVLTLDATD PLSKETDFKK CAVKLAKV 

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References

[1]"Complete sequence of Pectobacterium carotovorum subsp. carotovorum PC1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Balakrishnan V., Glasner J., Perna N.T.
Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PC1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001657 Genomic DNA. Translation: ACT13442.1.
RefSeqYP_003017978.1. NC_012917.1.

3D structure databases

ProteinModelPortalC6DK59.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING561230.PC1_2411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACT13442; ACT13442; PC1_2411.
GeneID8133357.
KEGGpct:PC1_2411.
PATRIC20489484. VBIPecCar70489_2426.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAQMVYNLH.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycPCAR561230:GKCK-2458-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PECCP
AccessionPrimary (citable) accession number: C6DK59
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families