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C6DK59

- NAPA_PECCP

UniProt

C6DK59 - NAPA_PECCP

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Protein
Periplasmic nitrate reductase
Gene
napA, PC1_2411
Organism
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPCAR561230:GKCK-2458-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:PC1_2411
OrganismiPectobacterium carotovorum subsp. carotovorum (strain PC1)
Taxonomic identifieri561230 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000002736: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signal Reviewed prediction
Add
BLAST
Chaini32 – 828797Periplasmic nitrate reductaseUniRule annotation
PRO_5000486441Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi561230.PC1_2411.

Structurei

3D structure databases

ProteinModelPortaliC6DK59.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiQMVYNLH.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C6DK59-1 [UniParc]FASTAAdd to Basket

« Hide

MKLSRRHFMK ANAVAAAAAV AGITIPIAVR AATEQSEAIH WDKAPCRFCG    50
VGCGVLVGTQ NGRIVASQGD PDAPVNRGLN CIKGYFLPKI MYGQDRLTQP 100
LLRMRDGKFD KEGEFTPISW DKAFDIMAEK FKTALKEKGP NAIGMFGSGQ 150
STIWEGYAAA KLFKAGFRSN NIDPNARHCM ASAVVGFMRT FGMDEPMGCY 200
DDIEQTDAFV LWGSNMAEMH PILWSRITDR RLSNSNVTVA VLSTYQHRSF 250
ELADNGMVFT PQTDLAILNY IANYIIQNNA VNEAFFTRHV NLRRGVTDIG 300
YGLRPTHPLE KAAKNPGSDA SEPMSFEEYK AFVADYTLEK TVAISGVPAD 350
QLEALAKLYA DPKKKVISYW TMGFNQHTRG VWANNLVYNI HLLTGKISQP 400
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRA IAEKLWQLPT 450
GTIPEKIGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NQERMPGWRD 500
PRNFIVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK 550
APGESKSDLW QVVSFAKRFA VEEVWPEELL AQKPAYRGKT LYDVLFANDV 600
TTRFPLSELA ENQLNDESRD FGFYLQKGLF EEYAAFGRGH GHDLAPFDDY 650
HKARGLRWPV VNGKETQWRY SEGHDPYVKA GEAYRFYGKP DGKAVIFALP 700
YEPAAEAPDD EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP 750
QDAKARDLRR GEKVRIISRR GEVVSVVETR GRNKPPRGLV YMPFFDAAQM 800
TNVLTLDATD PLSKETDFKK CAVKLAKV 828
Length:828
Mass (Da):92,674
Last modified:September 1, 2009 - v1
Checksum:iEF943C87CD7EE25B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001657 Genomic DNA. Translation: ACT13442.1.
RefSeqiYP_003017978.1. NC_012917.1.

Genome annotation databases

EnsemblBacteriaiACT13442; ACT13442; PC1_2411.
GeneIDi8133357.
KEGGipct:PC1_2411.
PATRICi20489484. VBIPecCar70489_2426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001657 Genomic DNA. Translation: ACT13442.1 .
RefSeqi YP_003017978.1. NC_012917.1.

3D structure databases

ProteinModelPortali C6DK59.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561230.PC1_2411.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT13442 ; ACT13442 ; PC1_2411 .
GeneIDi 8133357.
KEGGi pct:PC1_2411.
PATRICi 20489484. VBIPecCar70489_2426.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi QMVYNLH.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci PCAR561230:GKCK-2458-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Pectobacterium carotovorum subsp. carotovorum PC1."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.
    , Balakrishnan V., Glasner J., Perna N.T.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PC1.

Entry informationi

Entry nameiNAPA_PECCP
AccessioniPrimary (citable) accession number: C6DK59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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