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C6DF04

- SYI_PECCP

UniProt

C6DF04 - SYI_PECCP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPCAR561230:GKCK-3734-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PC1_3653
OrganismiPectobacterium carotovorum subsp. carotovorum (strain PC1)
Taxonomic identifieri561230 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000002736: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000216242Add
BLAST

Proteomic databases

PRIDEiC6DF04.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi561230.PC1_3653.

Structurei

3D structure databases

ProteinModelPortaliC6DF04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6DF04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRNAKKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRAECRKYA AEQVAGQKAD FIRLGVLGDW
160 170 180 190 200
DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV
210 220 230 240 250
EYYDKTSPSI DVAFNASDVA AVLAKFGVSS VDGPVSLVIW TTTPWTLPAN
260 270 280 290 300
RAISLNAEFD YQLVQIDGQA LILAADLVES VMKRAGVTQW TVLGDCKGAD
310 320 330 340 350
LELLRFKHPF LSFDVPAILG DHVTLDAGTG AVHTAGGHGP DDYVISQKYN
360 370 380 390 400
LEIANPVGPN GCYLSGTYPE LDGKFVFKAN DLIVEILREK GMLLHVEKLQ
410 420 430 440 450
HSYPCCWRHK SPIIFRATPQ WFVSMDQKGL RKQSLSEIKG VQWIPDWGQA
460 470 480 490 500
RIEAMVANRP DWCISRQRTW GVPMSLFVHK ETEELHPRTA ELIEAVAKRV
510 520 530 540 550
EADGIQAWWD LDPADVLGAD ADNYVKVPDT LDVWFDSGST HASVVDVRPE
560 570 580 590 600
FGGHAADMYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT VSPQDVMNKL GADILRLWIG STDYSGEIAV SDEILKRSAD
660 670 680 690 700
AYRRIRNTAR FLLANLNGFD PQKDSVKPED MVVLDRWAVG CAKAAQEEIL
710 720 730 740 750
EAYESYDFHR VVQRLMQFCS IEMGSFYLDI IKDRQYTAKS DSVARRSCQT
760 770 780 790 800
ALYHISEALV RWMAPIMSFT ADEIWSYLPG KRAQYVFTEE WYDGLFGLDA
810 820 830 840 850
SETMNDAFWA DILKVRSEVN KVIEQARNDK RIGGSLEASV TLYADANLAG
860 870 880 890 900
KLNQLRQELH FALLTSKALV ERYENAPDSA QATELTGLKI ALSEAEGHKC
910 920 930
PRCWHYETDI GSNADHPEVC GRCATNVGGN GEERKFV
Length:937
Mass (Da):104,502
Last modified:September 1, 2009 - v1
Checksum:iAB25485AF292FC83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001657 Genomic DNA. Translation: ACT14668.1.
RefSeqiWP_015841784.1. NC_012917.1.
YP_003019204.1. NC_012917.1.

Genome annotation databases

EnsemblBacteriaiACT14668; ACT14668; PC1_3653.
GeneIDi8134633.
KEGGipct:PC1_3653.
PATRICi20492054. VBIPecCar70489_3677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001657 Genomic DNA. Translation: ACT14668.1 .
RefSeqi WP_015841784.1. NC_012917.1.
YP_003019204.1. NC_012917.1.

3D structure databases

ProteinModelPortali C6DF04.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561230.PC1_3653.

Proteomic databases

PRIDEi C6DF04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT14668 ; ACT14668 ; PC1_3653 .
GeneIDi 8134633.
KEGGi pct:PC1_3653.
PATRICi 20492054. VBIPecCar70489_3677.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PCAR561230:GKCK-3734-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Pectobacterium carotovorum subsp. carotovorum PC1."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.
    , Balakrishnan V., Glasner J., Perna N.T.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PC1.

Entry informationi

Entry nameiSYI_PECCP
AccessioniPrimary (citable) accession number: C6DF04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: November 26, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3