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C6DF04

- SYI_PECCP

UniProt

C6DF04 - SYI_PECCP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPCAR561230:GKCK-3734-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PC1_3653
    OrganismiPectobacterium carotovorum subsp. carotovorum (strain PC1)
    Taxonomic identifieri561230 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
    ProteomesiUP000002736: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000216242Add
    BLAST

    Proteomic databases

    PRIDEiC6DF04.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi561230.PC1_3653.

    Structurei

    3D structure databases

    ProteinModelPortaliC6DF04.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6DF04-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRNAKKGKKT    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL 100
    PIELKVEQLI GKPGEKVSAA EFRAECRKYA AEQVAGQKAD FIRLGVLGDW 150
    DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV 200
    EYYDKTSPSI DVAFNASDVA AVLAKFGVSS VDGPVSLVIW TTTPWTLPAN 250
    RAISLNAEFD YQLVQIDGQA LILAADLVES VMKRAGVTQW TVLGDCKGAD 300
    LELLRFKHPF LSFDVPAILG DHVTLDAGTG AVHTAGGHGP DDYVISQKYN 350
    LEIANPVGPN GCYLSGTYPE LDGKFVFKAN DLIVEILREK GMLLHVEKLQ 400
    HSYPCCWRHK SPIIFRATPQ WFVSMDQKGL RKQSLSEIKG VQWIPDWGQA 450
    RIEAMVANRP DWCISRQRTW GVPMSLFVHK ETEELHPRTA ELIEAVAKRV 500
    EADGIQAWWD LDPADVLGAD ADNYVKVPDT LDVWFDSGST HASVVDVRPE 550
    FGGHAADMYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG 600
    RKMSKSIGNT VSPQDVMNKL GADILRLWIG STDYSGEIAV SDEILKRSAD 650
    AYRRIRNTAR FLLANLNGFD PQKDSVKPED MVVLDRWAVG CAKAAQEEIL 700
    EAYESYDFHR VVQRLMQFCS IEMGSFYLDI IKDRQYTAKS DSVARRSCQT 750
    ALYHISEALV RWMAPIMSFT ADEIWSYLPG KRAQYVFTEE WYDGLFGLDA 800
    SETMNDAFWA DILKVRSEVN KVIEQARNDK RIGGSLEASV TLYADANLAG 850
    KLNQLRQELH FALLTSKALV ERYENAPDSA QATELTGLKI ALSEAEGHKC 900
    PRCWHYETDI GSNADHPEVC GRCATNVGGN GEERKFV 937
    Length:937
    Mass (Da):104,502
    Last modified:September 1, 2009 - v1
    Checksum:iAB25485AF292FC83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001657 Genomic DNA. Translation: ACT14668.1.
    RefSeqiYP_003019204.1. NC_012917.1.

    Genome annotation databases

    EnsemblBacteriaiACT14668; ACT14668; PC1_3653.
    GeneIDi8134633.
    KEGGipct:PC1_3653.
    PATRICi20492054. VBIPecCar70489_3677.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001657 Genomic DNA. Translation: ACT14668.1 .
    RefSeqi YP_003019204.1. NC_012917.1.

    3D structure databases

    ProteinModelPortali C6DF04.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561230.PC1_3653.

    Proteomic databases

    PRIDEi C6DF04.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT14668 ; ACT14668 ; PC1_3653 .
    GeneIDi 8134633.
    KEGGi pct:PC1_3653.
    PATRICi 20492054. VBIPecCar70489_3677.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PCAR561230:GKCK-3734-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Pectobacterium carotovorum subsp. carotovorum PC1."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.
      , Balakrishnan V., Glasner J., Perna N.T.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PC1.

    Entry informationi

    Entry nameiSYI_PECCP
    AccessioniPrimary (citable) accession number: C6DF04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3