C6D3H2 (C6D3H2_PAESJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase 2 HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE2 HAMAP MF_00193
Ordered Locus Names:	Pjdr2_5510

Organism

Paenibacillus sp. (strain JDR-2) [Complete proteome] [HAMAP] EMBL ACT04119.1

Taxonomic identifier

324057 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 NAD HAMAP MF_00193 SAAS SAAS003694 Nucleotide-binding
Molecular function	Ligase SAAS SAAS003694 HAMAP MF_00193
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	47	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	C6D3H2 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 98724FA11579F275	FASTA	279	31,507
	10 20 30 40 50 60 MTLQQQIIEK LGVKPQIDAQ EEIRRTIDFI KDYMTFARKT ALVLGISGGQ DSTLAGKLCQ 70 80 90 100 110 120 MAVNELNDEE GVKTYRFIPV RLPYGNQKDE QDAQDAIAFI GAEGFITINI KAAVDAGAAE 130 140 150 160 170 180 YEARTGKKLS DFTKGNRKAR ERMIAQFEIA AEENGLVVGT DHAAEAITGF YTKFGDGAAD 190 200 210 220 230 240 LTPLYRLNKR QGRQILKELN CPEHLYLKAP TADLEEDKPQ LADEVALGVT YDDIDNYLEG 250 260 270 KEIGEDERQK IENWYIRTIH KREQPVTVFD RWWKKVDGK

References

[1]

"Complete sequence of Paenibacillus sp. JDR-2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Shanmugam K.T., Ingram L.O., Preston J. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JDR-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001656 Genomic DNA. Translation: ACT04119.1.
RefSeq	YP_003014205.1. NC_012914.1.
3D structure databases
ProteinModelPortal	C6D3H2.
SMR	C6D3H2. Positions 2-275.
ModBase	Search...
Protein-protein interaction databases
STRING	C6D3H2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8126421.
GenomeReviews	Gene locus Pjdr2_5510 in contig CP001656_GR.
KEGG	pjd:Pjdr2_5510.
PATRIC	22841507. VBIPaeSp118865_5455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAQYEIA.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6D3H2_PAESJ

Accession

Primary (citable) accession number: C6D3H2

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)