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C6CN33 (C6CN33_DICZE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase HAMAP-Rule MF_01687
Short name=Beta-gal HAMAP-Rule MF_01687
EC=3.2.1.23 HAMAP-Rule MF_01687
Alternative name(s):
Lactase HAMAP-Rule MF_01687
Gene names
Name:lacZ HAMAP-Rule MF_01687
Ordered Locus Names:Dd1591_2733
OrganismDickeya zeae (strain Ech1591) [Complete proteome] [HAMAP] EMBL ACT07558.1
Taxonomic identifier561229 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya ›

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. SAAS SAAS013812 HAMAP-Rule MF_01687

Cofactor

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family. HAMAP-Rule MF_01687

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region546 – 5494Substrate binding By similarity HAMAP-Rule MF_01687

Sites

Active site4701Proton donor By similarity HAMAP-Rule MF_01687
Active site5461Nucleophile By similarity HAMAP-Rule MF_01687
Metal binding2091Sodium By similarity HAMAP-Rule MF_01687
Metal binding4251Magnesium 1 By similarity HAMAP-Rule MF_01687
Metal binding4271Magnesium 1 By similarity HAMAP-Rule MF_01687
Metal binding4701Magnesium 1 By similarity HAMAP-Rule MF_01687
Metal binding6051Magnesium 2 By similarity HAMAP-Rule MF_01687
Metal binding6091Sodium; via carbonyl oxygen By similarity HAMAP-Rule MF_01687
Metal binding6121Sodium By similarity HAMAP-Rule MF_01687
Binding site1101Substrate By similarity HAMAP-Rule MF_01687
Binding site2091Substrate By similarity HAMAP-Rule MF_01687
Binding site4701Substrate By similarity HAMAP-Rule MF_01687
Binding site6121Substrate By similarity HAMAP-Rule MF_01687
Binding site10121Substrate By similarity HAMAP-Rule MF_01687
Site3661Transition state stabilizer By similarity HAMAP-Rule MF_01687
Site4001Transition state stabilizer By similarity HAMAP-Rule MF_01687

Sequences

Sequence LengthMass (Da)Tools
C6CN33 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: A8794CDE12419D1C

FASTA1,036118,909
        10         20         30         40         50         60 
MSTASAYAPL SGLSLADILA RRDWENPACP HIRRLDAHPP FSSWRNLNAA RDDQPSDRRQ 

        70         80         90        100        110        120 
MLNGEWTFSY FSRPEAVPEQ WLTQDLTDAN PLAVPSNWQL AGYDAPIYTN IKYPIPVNPP 

       130        140        150        160        170        180 
FVPQDNPTGC YSLTFSVNGD WLTQGQTRIV FDGVNSAFHL WCNGQWVGYS QDSRLPAEFD 

       190        200        210        220        230        240 
LTSCLQPGEN RLAVMVLRWS DGTYLEDQDM WRMSGIYRDV YLLHKPAVHL RDVQLTTPLR 

       250        260        270        280        290        300 
HSYTQGTLCV TALANLPEDQ AQAWQLAVQL WRGGQLVGEQ RAPFGTPAID ERGAYHDRVS 

       310        320        330        340        350        360 
LQLEVAQPAL WSAEEPNLYR AVVALEYDGT LVEAEAYDVG FREVAIRNGL LLLNGQPLLI 

       370        380        390        400        410        420 
RGTNRHEHHP QYGQAIDEAT MRQDILLMKQ HNFNAVRCSH YPNHPLWYRL CDRYGLYVVD 

       430        440        450        460        470        480 
EANIETHGMQ PMSRLSDDPR WLPAYAERVT RMVQRDRNHP CIIIWSLGNE SGYGPTHSAL 

       490        500        510        520        530        540 
YQWVKQQDPT RPVQYEGGGA DTPATDILCP MYARVDQDQP FPAVPKWSIK KWIGLPGEHR 

       550        560        570        580        590        600 
PLILCEYAHA MGNSFGGFDR YWQAFRQYPR LQGGFVWDWV DQALTREQDG KTHWAYGGDF 

       610        620        630        640        650        660 
GDKPNDRQFC LNGLVFPDRT PHPALYEAQR AQQFFQFNHH ENAPLTLTIT SEYLFRRSDN 

       670        680        690        700        710        720 
EELHWRIMQD DVQLASGRVP LDITPQGCQT LTLLEQLPAP QHHADMWLTV EVIQPNATDW 

       730        740        750        760        770        780 
SPAGHRCAWD QWQLPMPLAR PTPRRDGSER PTLTQNDDQF EVIHGQQRWA FNRHNGLLTQ 

       790        800        810        820        830        840 
WWRDGQAQLL SPLQDNLARA PLDNDIGISE VDRIDPNAWV ERWKLAGLYR YDTDCRHIHA 

       850        860        870        880        890        900 
DTLSDGVLIT TEHIGHYQQQ VLFISRKQWR IDAQGVLTVS VEVDVARHLP SLARVGLSMQ 

       910        920        930        940        950        960 
LAAVTPQVSW LGLGPHENYP DRRLAALHGR WQQPLEAMHT PYIFPSENGL RCHTRELRYG 

       970        980        990       1000       1010       1020 
DWLIEGDFHF GIGRYSRQQL MDCTHHHLLQ PEPGTWLNLD GFHMGIGGDD SWSPSVAPDF 

      1030 
LLTAPRYRYQ LQLQLR

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References

[1]"Complete sequence of Dickeya zeae Ech1591."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Balakrishnan V., Glasner J., Perna N.T.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ech1591.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001655 Genomic DNA. Translation: ACT07558.1.
RefSeqYP_003005037.1. NC_012912.1.

3D structure databases

ProteinModelPortalC6CN33.
ModBaseSearch...

Protein-protein interaction databases

STRING561229.Dd1591_2733.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACT07558; ACT07558; Dd1591_2733.
GeneID8119175.
KEGGdze:Dd1591_2733.
PATRIC21801475. VBIDicZea111179_2781.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMAFFRAPLD.
ProtClustDBPRK09525.

Enzyme and pathway databases

BioCycDZEA561229:GJ85-2791-MONOMER.

Family and domain databases

Gene3D2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF74650. Gal_mut_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 2 hits.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC6CN33_DICZE
AccessionPrimary (citable) accession number: C6CN33
Entry history
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info