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Protein

Beta-galactosidase

Gene

lacZ

Organism
Dickeya zeae (strain Ech1591)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110SubstrateUniRule annotation1
Metal bindingi209SodiumUniRule annotation1
Binding sitei209SubstrateUniRule annotation1
Sitei366Transition state stabilizerUniRule annotation1
Sitei400Transition state stabilizerUniRule annotation1
Metal bindingi425Magnesium 1UniRule annotation1
Metal bindingi427Magnesium 1UniRule annotation1
Active sitei470Proton donorUniRule annotation1
Metal bindingi470Magnesium 1UniRule annotation1
Binding sitei470SubstrateUniRule annotation1
Active sitei546NucleophileUniRule annotation1
Metal bindingi605Magnesium 2UniRule annotation1
Metal bindingi609Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi612SodiumUniRule annotation1
Binding sitei612SubstrateUniRule annotation1
Binding sitei1012SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, SodiumUniRule annotation

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:Dd1591_2733Imported
OrganismiDickeya zeae (strain Ech1591)Imported
Taxonomic identifieri561229 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya
Proteomesi
  • UP000002735 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi561229.Dd1591_2733.

Structurei

3D structure databases

ProteinModelPortaliC6CN33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini761 – 1034Bgal_small_NInterPro annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni546 – 549Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiAPIDNDM.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6CN33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTASAYAPL SGLSLADILA RRDWENPACP HIRRLDAHPP FSSWRNLNAA
60 70 80 90 100
RDDQPSDRRQ MLNGEWTFSY FSRPEAVPEQ WLTQDLTDAN PLAVPSNWQL
110 120 130 140 150
AGYDAPIYTN IKYPIPVNPP FVPQDNPTGC YSLTFSVNGD WLTQGQTRIV
160 170 180 190 200
FDGVNSAFHL WCNGQWVGYS QDSRLPAEFD LTSCLQPGEN RLAVMVLRWS
210 220 230 240 250
DGTYLEDQDM WRMSGIYRDV YLLHKPAVHL RDVQLTTPLR HSYTQGTLCV
260 270 280 290 300
TALANLPEDQ AQAWQLAVQL WRGGQLVGEQ RAPFGTPAID ERGAYHDRVS
310 320 330 340 350
LQLEVAQPAL WSAEEPNLYR AVVALEYDGT LVEAEAYDVG FREVAIRNGL
360 370 380 390 400
LLLNGQPLLI RGTNRHEHHP QYGQAIDEAT MRQDILLMKQ HNFNAVRCSH
410 420 430 440 450
YPNHPLWYRL CDRYGLYVVD EANIETHGMQ PMSRLSDDPR WLPAYAERVT
460 470 480 490 500
RMVQRDRNHP CIIIWSLGNE SGYGPTHSAL YQWVKQQDPT RPVQYEGGGA
510 520 530 540 550
DTPATDILCP MYARVDQDQP FPAVPKWSIK KWIGLPGEHR PLILCEYAHA
560 570 580 590 600
MGNSFGGFDR YWQAFRQYPR LQGGFVWDWV DQALTREQDG KTHWAYGGDF
610 620 630 640 650
GDKPNDRQFC LNGLVFPDRT PHPALYEAQR AQQFFQFNHH ENAPLTLTIT
660 670 680 690 700
SEYLFRRSDN EELHWRIMQD DVQLASGRVP LDITPQGCQT LTLLEQLPAP
710 720 730 740 750
QHHADMWLTV EVIQPNATDW SPAGHRCAWD QWQLPMPLAR PTPRRDGSER
760 770 780 790 800
PTLTQNDDQF EVIHGQQRWA FNRHNGLLTQ WWRDGQAQLL SPLQDNLARA
810 820 830 840 850
PLDNDIGISE VDRIDPNAWV ERWKLAGLYR YDTDCRHIHA DTLSDGVLIT
860 870 880 890 900
TEHIGHYQQQ VLFISRKQWR IDAQGVLTVS VEVDVARHLP SLARVGLSMQ
910 920 930 940 950
LAAVTPQVSW LGLGPHENYP DRRLAALHGR WQQPLEAMHT PYIFPSENGL
960 970 980 990 1000
RCHTRELRYG DWLIEGDFHF GIGRYSRQQL MDCTHHHLLQ PEPGTWLNLD
1010 1020 1030
GFHMGIGGDD SWSPSVAPDF LLTAPRYRYQ LQLQLR
Length:1,036
Mass (Da):118,909
Last modified:September 1, 2009 - v1
Checksum:iA8794CDE12419D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001655 Genomic DNA. Translation: ACT07558.1.

Genome annotation databases

EnsemblBacteriaiACT07558; ACT07558; Dd1591_2733.
KEGGidze:Dd1591_2733.
PATRICi21801475. VBIDicZea111179_2781.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001655 Genomic DNA. Translation: ACT07558.1.

3D structure databases

ProteinModelPortaliC6CN33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561229.Dd1591_2733.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT07558; ACT07558; Dd1591_2733.
KEGGidze:Dd1591_2733.
PATRICi21801475. VBIDicZea111179_2781.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiAPIDNDM.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC6CN33_DICZE
AccessioniPrimary (citable) accession number: C6CN33
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.