C6CLS9 (C6CLS9_DICZE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Dd1591_0836

Organism

Dickeya zeae (strain Ech1591) [Complete proteome] [HAMAP] EMBL ACT05715.1

Taxonomic identifier

561229 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Subunit structure	Homodimer By similarity. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 EMBL ACT05715.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	23	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	30	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	158	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	214	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	C6CLS9 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 88F1A254C0C8F375	FASTA	235	25,914
	10 20 30 40 50 60 MSTPNHALAE VVAVLPAAGN GSRMQSDRPK QYLSIGNKTI LEHTVAALLR HPRIHRVVIA 70 80 90 100 110 120 ISADDPYFSA LPLASDPRIQ VVTGGRQRAD SVLAGLQHVR QADWVLVHDA ARPCLHQDDL 130 140 150 160 170 180 SRLLLLTGQS EVGGILAAPV RDTMKRSRDG RIDHTVEREA LWHALTPQLF PLALLRESLE 190 200 210 220 230 RALREGATIT DEASALEYCG YRPQIVPGRA DNIKVTRPED LALAEFYLTR SPDHD

References

[1]

"Complete sequence of Dickeya zeae Ech1591." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Balakrishnan V., Glasner J., Perna N.T. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ech1591.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001655 Genomic DNA. Translation: ACT05715.1.
RefSeq	YP_003003194.1. NC_012912.1.
3D structure databases
ProteinModelPortal	C6CLS9.
ModBase	Search...
Protein-protein interaction databases
STRING	C6CLS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8120652.
GenomeReviews	Gene locus Dd1591_0836 in contig CP001655_GR.
KEGG	dze:Dd1591_0836.
PATRIC	21797560. VBIDicZea111179_0863.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SKVIVVC.
ProtClustDB	CLSK2549321.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6CLS9_DICZE

Accession

Primary (citable) accession number: C6CLS9

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)