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C6CJ51

- C6CJ51_DICZE

UniProt

C6CJ51 - C6CJ51_DICZE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Dd1591_0548
Organism
Dickeya zeae (strain Ech1591)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei609 – 6091ATP By similarityUniRule annotation
Metal bindingi904 – 9041Zinc By similarityUniRule annotation
Metal bindingi907 – 9071Zinc By similarityUniRule annotation
Metal bindingi924 – 9241Zinc By similarityUniRule annotation
Metal bindingi927 – 9271Zinc By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding, ZincUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciDZEA561229:GJ85-567-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dd1591_0548Imported
OrganismiDickeya zeae (strain Ech1591)Imported
Taxonomic identifieri561229 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya
ProteomesiUP000002735: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi561229.Dd1591_0548.

Structurei

3D structure databases

ProteinModelPortaliC6CJ51.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6CJ51-1 [UniParc]FASTAAdd to Basket

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MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRGAKKGKKT    50
FILHDGPPYA NGNIHIGHSV NKILKDIIIK SKGLSGYDSP YVPGWDCHGL 100
PIELKVEQLV GKPGEKVSAA QFRAECRKYA AEQVAGQKKD FIRLGVLGDW 150
DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV 200
EYYDKTSPAI DVAFNAVDRA AVLAKFGLTA DAVDGDVALV IWTTTPWTLP 250
ANRAIALNAD IDYVLIQVAG KAIIVAQGLA DAVIKRVAGN DASLLLGHTV 300
KGSDLELLRF RHPFMGFDVP AILGDHVTLD AGTGAVHTAG GHGPDDYVIS 350
QKYQLEIANP VGPNGCYLAG TFPELDGLFV FKANDKIVEL LRERGALLHH 400
EKLQHSYPCC WRHKSPILFR ATPQWFVSMD QNGLRKQSLS EIKGVQWIPD 450
WGQARIESMV ANRPDWCISR QRTWGVPMSL FVHKETQALH PRTAELIEAV 500
AKRVEQDGIQ AWWDLNPADL LGAEAAEYEK VPDTLDVWFD SGSTHASVVD 550
VRPEFSGHAA DMYLEGSDQH RGWFMSSLMI STAIKGKAPY RQVLTHGFTV 600
DGQGRKMSKS IGNTVSPQDV MDKLGADILR LWIGSTDYSG EIAVSDEILK 650
RSADAYRRIR NTARFLLANL NGFDPAQHSV KPEDMVVLDR WAVGCAKAAQ 700
DEIVDAYESY DFHHVVQRLM QFCSIEMGSF YLDIIKDRQY TAKHDSVARR 750
SCQTALFHIA EALVRWMAPI MSFTADEIWA YLPGERAQFV FTEEWYDGLF 800
GLADSESMND TFWADMLNVR GEVNKVIEQA RNDKRIGGSL EAAVTLYADD 850
ALFAQLGRLQ GELHFALLTS KAHLAHYEDA PADAQQSELP GLKVVLTKAD 900
GEKCPRCWHY ETDIGSDAAH PDVCGRCATN VGGNGEERKF V 941
Length:941
Mass (Da):104,510
Last modified:September 1, 2009 - v1
Checksum:iB50EF4CDAB815911
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001655 Genomic DNA. Translation: ACT05430.1.
RefSeqiYP_003002909.1. NC_012912.1.

Genome annotation databases

EnsemblBacteriaiACT05430; ACT05430; Dd1591_0548.
GeneIDi8119831.
KEGGidze:Dd1591_0548.
PATRICi21796948. VBIDicZea111179_0555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001655 Genomic DNA. Translation: ACT05430.1 .
RefSeqi YP_003002909.1. NC_012912.1.

3D structure databases

ProteinModelPortali C6CJ51.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561229.Dd1591_0548.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT05430 ; ACT05430 ; Dd1591_0548 .
GeneIDi 8119831.
KEGGi dze:Dd1591_0548.
PATRICi 21796948. VBIDicZea111179_0555.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DZEA561229:GJ85-567-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ech1591Imported.

Entry informationi

Entry nameiC6CJ51_DICZE
AccessioniPrimary (citable) accession number: C6CJ51
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi