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C6CJ51

- C6CJ51_DICZE

UniProt

C6CJ51 - C6CJ51_DICZE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dickeya zeae (strain Ech1591)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
Binding sitei609 – 6091ATPUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciDZEA561229:GJ85-567-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dd1591_0548Imported
OrganismiDickeya zeae (strain Ech1591)Imported
Taxonomic identifieri561229 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya
ProteomesiUP000002735: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi561229.Dd1591_0548.

Structurei

3D structure databases

ProteinModelPortaliC6CJ51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6CJ51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRGAKKGKKT
60 70 80 90 100
FILHDGPPYA NGNIHIGHSV NKILKDIIIK SKGLSGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQLV GKPGEKVSAA QFRAECRKYA AEQVAGQKKD FIRLGVLGDW
160 170 180 190 200
DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV
210 220 230 240 250
EYYDKTSPAI DVAFNAVDRA AVLAKFGLTA DAVDGDVALV IWTTTPWTLP
260 270 280 290 300
ANRAIALNAD IDYVLIQVAG KAIIVAQGLA DAVIKRVAGN DASLLLGHTV
310 320 330 340 350
KGSDLELLRF RHPFMGFDVP AILGDHVTLD AGTGAVHTAG GHGPDDYVIS
360 370 380 390 400
QKYQLEIANP VGPNGCYLAG TFPELDGLFV FKANDKIVEL LRERGALLHH
410 420 430 440 450
EKLQHSYPCC WRHKSPILFR ATPQWFVSMD QNGLRKQSLS EIKGVQWIPD
460 470 480 490 500
WGQARIESMV ANRPDWCISR QRTWGVPMSL FVHKETQALH PRTAELIEAV
510 520 530 540 550
AKRVEQDGIQ AWWDLNPADL LGAEAAEYEK VPDTLDVWFD SGSTHASVVD
560 570 580 590 600
VRPEFSGHAA DMYLEGSDQH RGWFMSSLMI STAIKGKAPY RQVLTHGFTV
610 620 630 640 650
DGQGRKMSKS IGNTVSPQDV MDKLGADILR LWIGSTDYSG EIAVSDEILK
660 670 680 690 700
RSADAYRRIR NTARFLLANL NGFDPAQHSV KPEDMVVLDR WAVGCAKAAQ
710 720 730 740 750
DEIVDAYESY DFHHVVQRLM QFCSIEMGSF YLDIIKDRQY TAKHDSVARR
760 770 780 790 800
SCQTALFHIA EALVRWMAPI MSFTADEIWA YLPGERAQFV FTEEWYDGLF
810 820 830 840 850
GLADSESMND TFWADMLNVR GEVNKVIEQA RNDKRIGGSL EAAVTLYADD
860 870 880 890 900
ALFAQLGRLQ GELHFALLTS KAHLAHYEDA PADAQQSELP GLKVVLTKAD
910 920 930 940
GEKCPRCWHY ETDIGSDAAH PDVCGRCATN VGGNGEERKF V
Length:941
Mass (Da):104,510
Last modified:September 1, 2009 - v1
Checksum:iB50EF4CDAB815911
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001655 Genomic DNA. Translation: ACT05430.1.
RefSeqiWP_012768312.1. NC_012912.1.
YP_003002909.1. NC_012912.1.

Genome annotation databases

EnsemblBacteriaiACT05430; ACT05430; Dd1591_0548.
GeneIDi8119831.
KEGGidze:Dd1591_0548.
PATRICi21796948. VBIDicZea111179_0555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001655 Genomic DNA. Translation: ACT05430.1 .
RefSeqi WP_012768312.1. NC_012912.1.
YP_003002909.1. NC_012912.1.

3D structure databases

ProteinModelPortali C6CJ51.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 561229.Dd1591_0548.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT05430 ; ACT05430 ; Dd1591_0548 .
GeneIDi 8119831.
KEGGi dze:Dd1591_0548.
PATRICi 21796948. VBIDicZea111179_0555.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DZEA561229:GJ85-567-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ech1591Imported.

Entry informationi

Entry nameiC6CJ51_DICZE
AccessioniPrimary (citable) accession number: C6CJ51
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3