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C6CJ51

- C6CJ51_DICZE

UniProt

C6CJ51 - C6CJ51_DICZE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dickeya zeae (strain Ech1591)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei565 – 5651Aminoacyl-adenylateUniRule annotation
    Binding sitei609 – 6091ATPUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciDZEA561229:GJ85-567-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Dd1591_0548Imported
    OrganismiDickeya zeae (strain Ech1591)Imported
    Taxonomic identifieri561229 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya
    ProteomesiUP000002735: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi561229.Dd1591_0548.

    Structurei

    3D structure databases

    ProteinModelPortaliC6CJ51.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi606 – 6105"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6CJ51-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRGAKKGKKT    50
    FILHDGPPYA NGNIHIGHSV NKILKDIIIK SKGLSGYDSP YVPGWDCHGL 100
    PIELKVEQLV GKPGEKVSAA QFRAECRKYA AEQVAGQKKD FIRLGVLGDW 150
    DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV 200
    EYYDKTSPAI DVAFNAVDRA AVLAKFGLTA DAVDGDVALV IWTTTPWTLP 250
    ANRAIALNAD IDYVLIQVAG KAIIVAQGLA DAVIKRVAGN DASLLLGHTV 300
    KGSDLELLRF RHPFMGFDVP AILGDHVTLD AGTGAVHTAG GHGPDDYVIS 350
    QKYQLEIANP VGPNGCYLAG TFPELDGLFV FKANDKIVEL LRERGALLHH 400
    EKLQHSYPCC WRHKSPILFR ATPQWFVSMD QNGLRKQSLS EIKGVQWIPD 450
    WGQARIESMV ANRPDWCISR QRTWGVPMSL FVHKETQALH PRTAELIEAV 500
    AKRVEQDGIQ AWWDLNPADL LGAEAAEYEK VPDTLDVWFD SGSTHASVVD 550
    VRPEFSGHAA DMYLEGSDQH RGWFMSSLMI STAIKGKAPY RQVLTHGFTV 600
    DGQGRKMSKS IGNTVSPQDV MDKLGADILR LWIGSTDYSG EIAVSDEILK 650
    RSADAYRRIR NTARFLLANL NGFDPAQHSV KPEDMVVLDR WAVGCAKAAQ 700
    DEIVDAYESY DFHHVVQRLM QFCSIEMGSF YLDIIKDRQY TAKHDSVARR 750
    SCQTALFHIA EALVRWMAPI MSFTADEIWA YLPGERAQFV FTEEWYDGLF 800
    GLADSESMND TFWADMLNVR GEVNKVIEQA RNDKRIGGSL EAAVTLYADD 850
    ALFAQLGRLQ GELHFALLTS KAHLAHYEDA PADAQQSELP GLKVVLTKAD 900
    GEKCPRCWHY ETDIGSDAAH PDVCGRCATN VGGNGEERKF V 941
    Length:941
    Mass (Da):104,510
    Last modified:September 1, 2009 - v1
    Checksum:iB50EF4CDAB815911
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001655 Genomic DNA. Translation: ACT05430.1.
    RefSeqiYP_003002909.1. NC_012912.1.

    Genome annotation databases

    EnsemblBacteriaiACT05430; ACT05430; Dd1591_0548.
    GeneIDi8119831.
    KEGGidze:Dd1591_0548.
    PATRICi21796948. VBIDicZea111179_0555.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001655 Genomic DNA. Translation: ACT05430.1 .
    RefSeqi YP_003002909.1. NC_012912.1.

    3D structure databases

    ProteinModelPortali C6CJ51.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 561229.Dd1591_0548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT05430 ; ACT05430 ; Dd1591_0548 .
    GeneIDi 8119831.
    KEGGi dze:Dd1591_0548.
    PATRICi 21796948. VBIDicZea111179_0555.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DZEA561229:GJ85-567-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ech1591Imported.

    Entry informationi

    Entry nameiC6CJ51_DICZE
    AccessioniPrimary (citable) accession number: C6CJ51
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3