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Protein

DNA topoisomerase 3

Gene

topB

Organism
Dickeya chrysanthemi (strain Ech1591) (Dickeya zeae (strain Ech1591))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 2UniRule annotation1
Metal bindingi105Magnesium 2UniRule annotation1
Active sitei328O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:Dd1591_2208Imported
OrganismiDickeya chrysanthemi (strain Ech1591) (Dickeya zeae (strain Ech1591))Imported
Taxonomic identifieri561229 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya
Proteomesi
  • UP000002735 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei178Interaction with DNAUniRule annotation1
Sitei330Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi561229.Dd1591_2208

Structurei

3D structure databases

ProteinModelPortaliC6CIK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IYH Bacteria
COG0550 LUCA
HOGENOMiHOG000086848
KOiK03169
OMAiTYPRVDT
OrthoDBiPOG091H028X

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00953 Topoisom_3_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR003602 Topo_IA_DNA-bd_dom
IPR005738 TopoIII
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
TIGRFAMsiTIGR01056 topB, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

C6CIK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFIAEKPS LARAIADVLP KPHRRGDGFI ACGDQDTVTW CVGHLLEQAQ
60 70 80 90 100
PDAYDARYAR WSLTDLPIVP QKWLLQPRPS VSKQLNVIKK LLTQADEIIH
110 120 130 140 150
AGDPDREGQL LVDEVLEYLA LSEEKRQHVR RCLINDLNPQ AVERAVSRLR
160 170 180 190 200
ENREFVPLCV SALARSRADW LYGINMTRAY TLLGKNAGYE GVLSVGRVQT
210 220 230 240 250
PVLGLVVRRD EEIDNFVPRD YFEVKAHIVT PANERFVALW QPSESCEPYQ
260 270 280 290 300
DDEGRLLHRP LAEHVVKRIE GQPAWVTAYN DKRESETAPL PYSLSALQIE
310 320 330 340 350
AAKRFGLSAQ QVLDICQRLY ETHKLITYPR SDCRYLPEEH FAGRQAVLNA
360 370 380 390 400
IATHRPDLLP QPAVDPERRN RCWDDGKVDA HHAIIPTARS ARTSLTEQES
410 420 430 440 450
QVYGLVARQY LMQFCADAVF RKCVIDLDIA GGKFVAKARF LAEAGWRTLL
460 470 480 490 500
GNKERDEENE GMPLPVVAKG DELLCERGEV VERQTQPPRP FTDATLLSAM
510 520 530 540 550
TGIARFVQDK ALKKILRETD GLGTEATRAG IIELLFKRAF LIKKGRYIHS
560 570 580 590 600
TDAGKALIHA LPPIAAHPDM TAHWEATLTQ ISEKRCRYQD FMQPLTQSLY
610 620 630 640
ELIQQARQSG NIQAFRGLPA VKRATPEKRK RRQSRSASTK E
Length:641
Mass (Da):72,600
Last modified:September 1, 2009 - v1
Checksum:i0A5650EDC3767B2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001655 Genomic DNA Translation: ACT07051.1

Genome annotation databases

EnsemblBacteriaiACT07051; ACT07051; Dd1591_2208
KEGGidze:Dd1591_2208

Similar proteinsi

Entry informationi

Entry nameiC6CIK6_DICC1
AccessioniPrimary (citable) accession number: C6CIK6
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: February 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health