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Protein

Glycerol kinase

Gene

glpK

Organism
Dickeya paradisiaca (strain Ech703) (Dickeya dadantii (strain Ech703))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotationSAAS annotation

Enzyme regulationi

Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system.UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateUniRule annotation1
Binding sitei19ATPUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Binding sitei269ATPUniRule annotation1
Binding sitei312ATP; via carbonyl oxygenUniRule annotation1
Binding sitei316ATP; via amide nitrogenUniRule annotation1
Binding sitei331ATPUniRule annotation1
Metal bindingi480Zinc; shared with EIIA-GlcUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 17ATPUniRule annotation3
Nucleotide bindingi413 – 417ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, KinaseUniRule annotationSAAS annotationImported, Transferase
Biological processGlycerol metabolismUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:Dd703_3803Imported
OrganismiDickeya paradisiaca (strain Ech703) (Dickeya dadantii (strain Ech703))Imported
Taxonomic identifieri579405 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeDickeya
Proteomesi
  • UP000002734 Componenti: Chromosome

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.UniRule annotation

Protein-protein interaction databases

STRINGi579405.Dd703_3803

Structurei

3D structure databases

ProteinModelPortaliC6C5B9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 254FGGY_NInterPro annotationAdd BLAST248
Domaini263 – 452FGGY_CInterPro annotationAdd BLAST190

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Substrate bindingUniRule annotation2
Regioni235 – 237Allosteric FBP inhibitor bindingUniRule annotation3
Regioni247 – 248Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4107QVN Bacteria
COG0554 LUCA
HOGENOMiHOG000222134
KOiK00864
OMAiWQDTRTQ
OrthoDBiPOG091H02FA

Family and domain databases

HAMAPiMF_00186 Glycerol_kin, 1 hit
InterProiView protein in InterPro
IPR000577 Carb_kinase_FGGY
IPR018485 Carb_kinase_FGGY_C
IPR018483 Carb_kinase_FGGY_CS
IPR018484 Carb_kinase_FGGY_N
IPR005999 Glycerol_kin
PfamiView protein in Pfam
PF02782 FGGY_C, 1 hit
PF00370 FGGY_N, 1 hit
PIRSFiPIRSF000538 GlpK, 1 hit
TIGRFAMsiTIGR01311 glycerol_kin, 1 hit
PROSITEiView protein in PROSITE
PS00933 FGGY_KINASES_1, 1 hit
PS00445 FGGY_KINASES_2, 1 hit

Sequencei

Sequence statusi: Complete.

C6C5B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEKKYIVA LDQGTTSSRA VVLDHDANIV SVSQREFPQI YPKPGWVEHD
60 70 80 90 100
PMEIWASQSS TLVEALAKAG ISSDEVAGIG ITNQRETVVV WEKETGKPIY
110 120 130 140 150
NAIVWQCRRT ADICEKLKKD GLEEYIRANT GLVVDPYFSG TKVKWILDHV
160 170 180 190 200
DGARDRANRG ELLLGTIDTW LIWKMTQGRV HVTDYTNASR TMLFNIHKLD
210 220 230 240 250
WDERMLEALD IPRSMLPQVR PSSEMYGQTN IGGKGGTRIP ICGIAGDQQA
260 270 280 290 300
ALYGQLCVQP GMAKNTYGTG CFLLMNTGTE AVASRHGLLT TIACGPRGEV
310 320 330 340 350
NYALEGAVFI GGASIQWLRD ELKLINDAAD SEYFATKVKD TNGVYVVPAF
360 370 380 390 400
TGLGAPYWDP YARGAIFGLT RGANANHIIR ATLESIAFQT RDVLDAMQAD
410 420 430 440 450
ADTRLQSLRV DGGAVANNFL MQFQSDILGT RVERPQVRES TALGAAFLAG
460 470 480 490 500
LATGFWNDLD EVKSKTAIER EFRPSIETVE RNFRYRGWQK AVERARNWED

HDA
Length:503
Mass (Da):55,776
Last modified:September 1, 2009 - v1
Checksum:i5825E56251D1F4B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001654 Genomic DNA Translation: ACS87556.1

Genome annotation databases

EnsemblBacteriaiACS87556; ACS87556; Dd703_3803
KEGGidda:Dd703_3803

Similar proteinsi

Entry informationi

Entry nameiC6C5B9_DICP7
AccessioniPrimary (citable) accession number: C6C5B9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: April 25, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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