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Protein

Glycerol kinase

Gene

glpK

Organism
Dickeya dadantii (strain Ech703)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotationSAAS annotation

Enzyme regulationi

Activity of this regulatory enzyme is affected by several metabolites. Allosterically and non-competitively inhibited by fructose 1,6-bisphosphate (FBP) and unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151SubstrateUniRule annotation
Binding sitei19 – 191ATPUniRule annotation
Binding sitei137 – 1371SubstrateUniRule annotation
Binding sitei269 – 2691ATPUniRule annotation
Binding sitei312 – 3121ATP; via carbonyl oxygenUniRule annotation
Binding sitei316 – 3161ATP; via amide nitrogenUniRule annotation
Binding sitei331 – 3311ATPUniRule annotation
Metal bindingi480 – 4801Zinc; shared with EIIA-GlcUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 173ATPUniRule annotation
Nucleotide bindingi413 – 4175ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glycerol kinase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: UniProtKB-HAMAP
  2. glycerol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

Glycerol metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Enzyme and pathway databases

BioCyciDDAD579405:GHJU-3897-MONOMER.
UniPathwayiUPA00618; UER00672.
UPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotationSAAS annotation (EC:2.7.1.30UniRule annotationSAAS annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:Dd703_3803Imported
OrganismiDickeya dadantii (strain Ech703)Imported
Taxonomic identifieri579405 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeDickeya
ProteomesiUP000002734: Chromosome

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc. Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization.UniRule annotation

Protein-protein interaction databases

STRINGi579405.Dd703_3803.

Structurei

3D structure databases

ProteinModelPortaliC6C5B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 862Substrate bindingUniRule annotation
Regioni235 – 2373Allosteric FBP inhibitor bindingUniRule annotation
Regioni247 – 2482Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0554.
HOGENOMiHOG000222134.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6C5B9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEKKYIVA LDQGTTSSRA VVLDHDANIV SVSQREFPQI YPKPGWVEHD
60 70 80 90 100
PMEIWASQSS TLVEALAKAG ISSDEVAGIG ITNQRETVVV WEKETGKPIY
110 120 130 140 150
NAIVWQCRRT ADICEKLKKD GLEEYIRANT GLVVDPYFSG TKVKWILDHV
160 170 180 190 200
DGARDRANRG ELLLGTIDTW LIWKMTQGRV HVTDYTNASR TMLFNIHKLD
210 220 230 240 250
WDERMLEALD IPRSMLPQVR PSSEMYGQTN IGGKGGTRIP ICGIAGDQQA
260 270 280 290 300
ALYGQLCVQP GMAKNTYGTG CFLLMNTGTE AVASRHGLLT TIACGPRGEV
310 320 330 340 350
NYALEGAVFI GGASIQWLRD ELKLINDAAD SEYFATKVKD TNGVYVVPAF
360 370 380 390 400
TGLGAPYWDP YARGAIFGLT RGANANHIIR ATLESIAFQT RDVLDAMQAD
410 420 430 440 450
ADTRLQSLRV DGGAVANNFL MQFQSDILGT RVERPQVRES TALGAAFLAG
460 470 480 490 500
LATGFWNDLD EVKSKTAIER EFRPSIETVE RNFRYRGWQK AVERARNWED

HDA
Length:503
Mass (Da):55,776
Last modified:September 1, 2009 - v1
Checksum:i5825E56251D1F4B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001654 Genomic DNA. Translation: ACS87556.1.
RefSeqiYP_002989378.1. NC_012880.1.

Genome annotation databases

EnsemblBacteriaiACS87556; ACS87556; Dd703_3803.
GeneIDi8087188.
KEGGidda:Dd703_3803.
PATRICi21795313. VBIDicDad95084_4030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001654 Genomic DNA. Translation: ACS87556.1.
RefSeqiYP_002989378.1. NC_012880.1.

3D structure databases

ProteinModelPortaliC6C5B9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi579405.Dd703_3803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS87556; ACS87556; Dd703_3803.
GeneIDi8087188.
KEGGidda:Dd703_3803.
PATRICi21795313. VBIDicDad95084_4030.

Phylogenomic databases

eggNOGiCOG0554.
HOGENOMiHOG000222134.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
UPA00618; UER00672.
BioCyciDDAD579405:GHJU-3897-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ech703Imported.

Entry informationi

Entry nameiC6C5B9_DICDC
AccessioniPrimary (citable) accession number: C6C5B9
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: February 4, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.