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C6BV89

- SYI_DESAD

UniProt

C6BV89 - SYI_DESAD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei571 – 5711Aminoacyl-adenylateUniRule annotation
    Binding sitei615 – 6151ATPUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDSAL526222:GHES-2039-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Desal_2004
    OrganismiDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
    Taxonomic identifieri526222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
    ProteomesiUP000002601: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000216233Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi526222.Desal_2004.

    Structurei

    3D structure databases

    ProteinModelPortaliC6BV89.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi612 – 6165"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6BV89-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKKTLCL PKTKFPMKAN LKQREPEMLK RWEETGVYEK MVEANAEAEQ    50
    YVLHDGPPYA NGHIHMGTAM NKVLKDIIVK SRNLQGMKAE YVPGWDCHGL 100
    PIEHKVEQEL KKKKKELPTT VIRKLCREYA AKFVDIQRKE FKRLGVLGNW 150
    EDPYLTMKPE YEAATARELG RFMEKGSVIR GKKPIHWCCD CRTALAEAEV 200
    EYEDHTSPSI YVRFPLNDEK VLKALPEDVA SKVDLSRTYV CIWTTTPWTI 250
    PDNMAVAVHP EFEYAVTEVN GDIYILAERL LPVCAESFGW ESWNVLATVA 300
    GAKLEGAVAK HPIYDRKSPV VLADYVTLDS GTGCVHTAPG HGREDFETGL 350
    KNGLEVYSPM NNSGVFLKEV EFFAGMNVWE ANPKVIEKLE EVGNLLAQEK 400
    ISHSYPHCWR CKEPVIFRAT TQWFIGMEEN DLRKKALKAI KDDVEWLPAW 450
    GENRIYSMVE NRPDWCISRQ RNWGVPIIAL ICEDCDEVYN DPEWVFSVVD 500
    EFEKHERGCD YWFEKSIEEI VPEGLKCPKC GGNHWTKEDD ILDVWFDSGT 550
    SFAAVVEKRK EHRFPADLYL EGSDQHRGWF HSSLLASVGT RDVPPYKTVL 600
    THGYVVDKDG RKMSKSIGNV IAPQEIIDQH GAEILRMWVS AVNYQEDVRI 650
    SDEILSRMVD TYRRVRNTCR YILGNLDGFN PETDAVAPAD MLPIDHFALD 700
    LVNRQHEVIQ KAYTNFEFHK VYHTLHNLCT TELSAFYLDI IKDRLYVSGE 750
    KSLERRSAQT VLWQTMLMLL KDMAPILSFT AEEAYSHMPE EMKGSAETVF 800
    AIRPELLKPS IDDAERKRWE LLMDVRTEVT KAIEPLRREK VIGHSLDTKI 850
    TLFANEEIAK ALEGIERREF FIVSGVEIKP LAEASDDAVK PEELEGLSIK 900
    VEKAQGEKCS RCWRYDTLGT NAEHPELCPR CAAVLAG 937
    Length:937
    Mass (Da):107,288
    Last modified:September 1, 2009 - v1
    Checksum:i00FEF119D2DC0E33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001649 Genomic DNA. Translation: ACS80064.1.
    RefSeqiYP_002991603.1. NC_012881.1.

    Genome annotation databases

    EnsemblBacteriaiACS80064; ACS80064; Desal_2004.
    GeneIDi8093434.
    KEGGidsa:Desal_2004.
    PATRICi21759517. VBIDesSal121003_2012.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001649 Genomic DNA. Translation: ACS80064.1 .
    RefSeqi YP_002991603.1. NC_012881.1.

    3D structure databases

    ProteinModelPortali C6BV89.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 526222.Desal_2004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS80064 ; ACS80064 ; Desal_2004 .
    GeneIDi 8093434.
    KEGGi dsa:Desal_2004.
    PATRICi 21759517. VBIDesSal121003_2012.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DSAL526222:GHES-2039-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763.

    Entry informationi

    Entry nameiSYI_DESAD
    AccessioniPrimary (citable) accession number: C6BV89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3