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C6BV89

- SYI_DESAD

UniProt

C6BV89 - SYI_DESAD

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Desal_2004
Organism
Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei571 – 5711Aminoacyl-adenylate By similarity
Binding sitei615 – 6151ATP By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi912 – 9121Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDSAL526222:GHES-2039-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Desal_2004
OrganismiDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
Taxonomic identifieri526222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000002601: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligaseUniRule annotationPRO_1000216233Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi526222.Desal_2004.

Structurei

3D structure databases

ProteinModelPortaliC6BV89.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi612 – 6165"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6BV89-1 [UniParc]FASTAAdd to Basket

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MSDYKKTLCL PKTKFPMKAN LKQREPEMLK RWEETGVYEK MVEANAEAEQ    50
YVLHDGPPYA NGHIHMGTAM NKVLKDIIVK SRNLQGMKAE YVPGWDCHGL 100
PIEHKVEQEL KKKKKELPTT VIRKLCREYA AKFVDIQRKE FKRLGVLGNW 150
EDPYLTMKPE YEAATARELG RFMEKGSVIR GKKPIHWCCD CRTALAEAEV 200
EYEDHTSPSI YVRFPLNDEK VLKALPEDVA SKVDLSRTYV CIWTTTPWTI 250
PDNMAVAVHP EFEYAVTEVN GDIYILAERL LPVCAESFGW ESWNVLATVA 300
GAKLEGAVAK HPIYDRKSPV VLADYVTLDS GTGCVHTAPG HGREDFETGL 350
KNGLEVYSPM NNSGVFLKEV EFFAGMNVWE ANPKVIEKLE EVGNLLAQEK 400
ISHSYPHCWR CKEPVIFRAT TQWFIGMEEN DLRKKALKAI KDDVEWLPAW 450
GENRIYSMVE NRPDWCISRQ RNWGVPIIAL ICEDCDEVYN DPEWVFSVVD 500
EFEKHERGCD YWFEKSIEEI VPEGLKCPKC GGNHWTKEDD ILDVWFDSGT 550
SFAAVVEKRK EHRFPADLYL EGSDQHRGWF HSSLLASVGT RDVPPYKTVL 600
THGYVVDKDG RKMSKSIGNV IAPQEIIDQH GAEILRMWVS AVNYQEDVRI 650
SDEILSRMVD TYRRVRNTCR YILGNLDGFN PETDAVAPAD MLPIDHFALD 700
LVNRQHEVIQ KAYTNFEFHK VYHTLHNLCT TELSAFYLDI IKDRLYVSGE 750
KSLERRSAQT VLWQTMLMLL KDMAPILSFT AEEAYSHMPE EMKGSAETVF 800
AIRPELLKPS IDDAERKRWE LLMDVRTEVT KAIEPLRREK VIGHSLDTKI 850
TLFANEEIAK ALEGIERREF FIVSGVEIKP LAEASDDAVK PEELEGLSIK 900
VEKAQGEKCS RCWRYDTLGT NAEHPELCPR CAAVLAG 937
Length:937
Mass (Da):107,288
Last modified:September 1, 2009 - v1
Checksum:i00FEF119D2DC0E33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001649 Genomic DNA. Translation: ACS80064.1.
RefSeqiYP_002991603.1. NC_012881.1.

Genome annotation databases

EnsemblBacteriaiACS80064; ACS80064; Desal_2004.
GeneIDi8093434.
KEGGidsa:Desal_2004.
PATRICi21759517. VBIDesSal121003_2012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001649 Genomic DNA. Translation: ACS80064.1 .
RefSeqi YP_002991603.1. NC_012881.1.

3D structure databases

ProteinModelPortali C6BV89.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 526222.Desal_2004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS80064 ; ACS80064 ; Desal_2004 .
GeneIDi 8093434.
KEGGi dsa:Desal_2004.
PATRICi 21759517. VBIDesSal121003_2012.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DSAL526222:GHES-2039-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763.

Entry informationi

Entry nameiSYI_DESAD
AccessioniPrimary (citable) accession number: C6BV89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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