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C6BV89

- SYI_DESAD

UniProt

C6BV89 - SYI_DESAD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei571 – 5711Aminoacyl-adenylateUniRule annotation
Binding sitei615 – 6151ATPUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDSAL526222:GHES-2039-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Desal_2004
OrganismiDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
Taxonomic identifieri526222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
ProteomesiUP000002601: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000216233Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi526222.Desal_2004.

Structurei

3D structure databases

ProteinModelPortaliC6BV89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi612 – 6165"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6BV89-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKKTLCL PKTKFPMKAN LKQREPEMLK RWEETGVYEK MVEANAEAEQ
60 70 80 90 100
YVLHDGPPYA NGHIHMGTAM NKVLKDIIVK SRNLQGMKAE YVPGWDCHGL
110 120 130 140 150
PIEHKVEQEL KKKKKELPTT VIRKLCREYA AKFVDIQRKE FKRLGVLGNW
160 170 180 190 200
EDPYLTMKPE YEAATARELG RFMEKGSVIR GKKPIHWCCD CRTALAEAEV
210 220 230 240 250
EYEDHTSPSI YVRFPLNDEK VLKALPEDVA SKVDLSRTYV CIWTTTPWTI
260 270 280 290 300
PDNMAVAVHP EFEYAVTEVN GDIYILAERL LPVCAESFGW ESWNVLATVA
310 320 330 340 350
GAKLEGAVAK HPIYDRKSPV VLADYVTLDS GTGCVHTAPG HGREDFETGL
360 370 380 390 400
KNGLEVYSPM NNSGVFLKEV EFFAGMNVWE ANPKVIEKLE EVGNLLAQEK
410 420 430 440 450
ISHSYPHCWR CKEPVIFRAT TQWFIGMEEN DLRKKALKAI KDDVEWLPAW
460 470 480 490 500
GENRIYSMVE NRPDWCISRQ RNWGVPIIAL ICEDCDEVYN DPEWVFSVVD
510 520 530 540 550
EFEKHERGCD YWFEKSIEEI VPEGLKCPKC GGNHWTKEDD ILDVWFDSGT
560 570 580 590 600
SFAAVVEKRK EHRFPADLYL EGSDQHRGWF HSSLLASVGT RDVPPYKTVL
610 620 630 640 650
THGYVVDKDG RKMSKSIGNV IAPQEIIDQH GAEILRMWVS AVNYQEDVRI
660 670 680 690 700
SDEILSRMVD TYRRVRNTCR YILGNLDGFN PETDAVAPAD MLPIDHFALD
710 720 730 740 750
LVNRQHEVIQ KAYTNFEFHK VYHTLHNLCT TELSAFYLDI IKDRLYVSGE
760 770 780 790 800
KSLERRSAQT VLWQTMLMLL KDMAPILSFT AEEAYSHMPE EMKGSAETVF
810 820 830 840 850
AIRPELLKPS IDDAERKRWE LLMDVRTEVT KAIEPLRREK VIGHSLDTKI
860 870 880 890 900
TLFANEEIAK ALEGIERREF FIVSGVEIKP LAEASDDAVK PEELEGLSIK
910 920 930
VEKAQGEKCS RCWRYDTLGT NAEHPELCPR CAAVLAG
Length:937
Mass (Da):107,288
Last modified:September 1, 2009 - v1
Checksum:i00FEF119D2DC0E33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001649 Genomic DNA. Translation: ACS80064.1.
RefSeqiYP_002991603.1. NC_012881.1.

Genome annotation databases

EnsemblBacteriaiACS80064; ACS80064; Desal_2004.
GeneIDi8093434.
KEGGidsa:Desal_2004.
PATRICi21759517. VBIDesSal121003_2012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001649 Genomic DNA. Translation: ACS80064.1 .
RefSeqi YP_002991603.1. NC_012881.1.

3D structure databases

ProteinModelPortali C6BV89.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 526222.Desal_2004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS80064 ; ACS80064 ; Desal_2004 .
GeneIDi 8093434.
KEGGi dsa:Desal_2004.
PATRICi 21759517. VBIDesSal121003_2012.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DSAL526222:GHES-2039-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763.

Entry informationi

Entry nameiSYI_DESAD
AccessioniPrimary (citable) accession number: C6BV89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: November 26, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3