C6BST2 (LEPA_DESAD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1

Alternative name(s):
Ribosomal back-translocase LepA

Gene names

Name:	lepA
Ordered Locus Names:	Desal_3490

Organism

Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763) [Complete proteome] [HAMAP]

Taxonomic identifier

526222 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio › Desulfovibrio salexigens

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Elongation factor 4 HAMAP-Rule MF_00071		PRO_1000202446
Regions
	Nucleotide binding	17 – 22	6	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C6BST2 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 07082EC120712C2A	FASTA	601	67,050
	10 20 30 40 50 60 MAKLDKIRNF SIIAHIDHGK STLADRILEI TGMVSEREKK DQYLDKMELE QERGITIKAQ 70 80 90 100 110 120 TVRIPYKAND GEEYILNLID TPGHVDFSYE VSRSLAASEG ALLVVDSTQG VEAQTLANVF 130 140 150 160 170 180 LALDHDLEIV PVLNKVDLPS SDCERVAQEI EEVIGLDCSE PLMISAKTGL NVEDVLESIV 190 200 210 220 230 240 KDLPAPQGDP DAPLKALIFD SWYDSYQGVV VLFRILDGTI KKGDKIQIHS TGRTFDVTTL 250 260 270 280 290 300 GVYTPEPQKA KELAAGEVGF LCASMKELND APVGDTITLA ADPVEDPFPG FQEVKPMVFC 310 320 330 340 350 360 GLYPVEPAEY EPLKAALEKL QLNDTAFSFE PETSTALGFG FRCGFLGLLH MEIIQERLER 370 380 390 400 410 420 EFQAKLIATA PSVVYQARLN NGEVLEIDNP SKMPDGGDLE SLAEPFCRLE IHVPNDYVGA 430 440 450 460 470 480 VLKLCEEKRG IQKDMRYLTS SRVIITYEVP FAEIMYDFFD KLKSHTKGYA SLDYEIIDYR 490 500 510 520 530 540 ESNLVKLDIL INTDPVDAFS AIVHKDSAYP FGRSLALKLK RAIPRQMFEI VIQAAIGRKI 550 560 570 580 590 600 IAKERVAPFR KNVTAKCYGG DITRKRKLLE KQKEGKKRMK KMGNVEIPQE AFMAVLKAGE D

References

[1]

"Complete sequence of Desulfovibrio salexigens DSM 2638." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I. , Wall J.D., Arkin A.P., Dehal P., Chivian D., Giles B., Hazen T.C. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001649 Genomic DNA. Translation: ACS81538.1.
RefSeq	YP_002993077.1. NC_012881.1.
3D structure databases
ProteinModelPortal	C6BST2.
ModBase	Search...
Protein-protein interaction databases
STRING	526222.Desal_3490.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS81538; ACS81538; Desal_3490.
GeneID	8092457.
KEGG	dsa:Desal_3490.
PATRIC	21762629. VBIDesSal121003_3500.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0481.
HOGENOM	HOG000020624.
KO	K03596.
ProtClustDB	PRK05433.
Enzyme and pathway databases
BioCyc	DSAL526222:GHES-3594-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPA_DESAD

Accession

Primary (citable) accession number: C6BST2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program