Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C6BJD4

- C6BJD4_RALP1

UniProt

C6BJD4 - C6BJD4_RALP1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Ralstonia pickettii (strain 12D)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme AUniRule annotation
Binding sitei512 – 5121ATPUniRule annotation
Binding sitei528 – 5281ATPUniRule annotation
Binding sitei536 – 5361Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei539 – 5391ATPUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi397 – 3993ATPUniRule annotation
Nucleotide bindingi421 – 4266ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC428406:GH9Y-1808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Rpic12D_1775Imported
OrganismiRalstonia pickettii (strain 12D)Imported
Taxonomic identifieri428406 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000008208: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi428406.Rpic12D_1775.

Structurei

3D structure databases

ProteinModelPortaliC6BJD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 2004Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiGGCEAVT.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6BJD4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGIESVLQE TRVFNPPESF VKQANIAGMD AYRALCAEAE KDYEGFWARL
60 70 80 90 100
AHEHLLWHKP FSKVLDESNA PFYKWFEDGE LNASYNCLER NLENGNADKV
110 120 130 140 150
AIIFETDDGN VSRITYRELH ARVCRFANGL KALGIKKGDR VVIYMPMSVE
160 170 180 190 200
GIVAMQACAR IGATHSVVFG GFSAKSLQER IVDVGAVALI TADEQMRGGK
210 220 230 240 250
ALPLKAIADE ALAMEGTGAV KHVIVYRRTN GNVNWVEGRD RAMDEVEAGQ
260 270 280 290 300
PDTCEVTPVS AEHPLFILYT SGSTGKPKGV QHSTGGYLLW ALLTMQWTFD
310 320 330 340 350
LKPDDIFWCT ADIGWVTGHS YIAYGPLAAG ATQVVFEGVP TYPNAGRFWD
360 370 380 390 400
MIQRHKVNTF YTAPTAIRSL IKAAEADEKV HPKQYDLSSL RLLGTVGEPI
410 420 430 440 450
NPEAWMWYHT NIGGGRCPIV DTFWQTETGG HMMTPLPGAT PLVPGSCTLP
460 470 480 490 500
LPGIMAAVVD ETGHDVPNGQ GGILVVKRPW PSMIRTIWGD PERFKKSYFP
510 520 530 540 550
EELGGKLYLA GDGSIRDKET GYFTIMGRID DVLNVSGHRM GTMEIESALV
560 570 580 590 600
ANPIVAEAAV VGRPDDMTGE AICAFVVLKR ARPDGDEAKQ IANELRNWVG
610 620 630 640 650
KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEDI TQDTSTLENP
660
AILDQLKETR
Length:660
Mass (Da):72,595
Last modified:September 1, 2009 - v1
Checksum:i5656BD9158B10654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001644 Genomic DNA. Translation: ACS63057.1.
RefSeqiWP_012762228.1. NC_012856.1.
YP_002981729.1. NC_012856.1.

Genome annotation databases

EnsemblBacteriaiACS63057; ACS63057; Rpic12D_1775.
GeneIDi8019431.
KEGGirpf:Rpic12D_1775.
PATRICi20242597. VBIRalPic57998_2388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001644 Genomic DNA. Translation: ACS63057.1 .
RefSeqi WP_012762228.1. NC_012856.1.
YP_002981729.1. NC_012856.1.

3D structure databases

ProteinModelPortali C6BJD4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 428406.Rpic12D_1775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS63057 ; ACS63057 ; Rpic12D_1775 .
GeneIDi 8019431.
KEGGi rpf:Rpic12D_1775.
PATRICi 20242597. VBIRalPic57998_2388.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi GGCEAVT.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci RPIC428406:GH9Y-1808-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12DImported.

Entry informationi

Entry nameiC6BJD4_RALP1
AccessioniPrimary (citable) accession number: C6BJD4
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 26, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3