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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Ralstonia pickettii (strain 12D)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme AUniRule annotation
Binding sitei512 – 5121ATPUniRule annotation
Binding sitei528 – 5281ATPUniRule annotation
Binding sitei536 – 5361Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei539 – 5391ATPUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi397 – 3993ATPUniRule annotation
Nucleotide bindingi421 – 4266ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciRPIC428406:GH9Y-1808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Rpic12D_1775Imported
OrganismiRalstonia pickettii (strain 12D)Imported
Taxonomic identifieri428406 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000008208 Componenti: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC6BJD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 2004Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiMRHTDCL.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6BJD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIESVLQE TRVFNPPESF VKQANIAGMD AYRALCAEAE KDYEGFWARL
60 70 80 90 100
AHEHLLWHKP FSKVLDESNA PFYKWFEDGE LNASYNCLER NLENGNADKV
110 120 130 140 150
AIIFETDDGN VSRITYRELH ARVCRFANGL KALGIKKGDR VVIYMPMSVE
160 170 180 190 200
GIVAMQACAR IGATHSVVFG GFSAKSLQER IVDVGAVALI TADEQMRGGK
210 220 230 240 250
ALPLKAIADE ALAMEGTGAV KHVIVYRRTN GNVNWVEGRD RAMDEVEAGQ
260 270 280 290 300
PDTCEVTPVS AEHPLFILYT SGSTGKPKGV QHSTGGYLLW ALLTMQWTFD
310 320 330 340 350
LKPDDIFWCT ADIGWVTGHS YIAYGPLAAG ATQVVFEGVP TYPNAGRFWD
360 370 380 390 400
MIQRHKVNTF YTAPTAIRSL IKAAEADEKV HPKQYDLSSL RLLGTVGEPI
410 420 430 440 450
NPEAWMWYHT NIGGGRCPIV DTFWQTETGG HMMTPLPGAT PLVPGSCTLP
460 470 480 490 500
LPGIMAAVVD ETGHDVPNGQ GGILVVKRPW PSMIRTIWGD PERFKKSYFP
510 520 530 540 550
EELGGKLYLA GDGSIRDKET GYFTIMGRID DVLNVSGHRM GTMEIESALV
560 570 580 590 600
ANPIVAEAAV VGRPDDMTGE AICAFVVLKR ARPDGDEAKQ IANELRNWVG
610 620 630 640 650
KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEDI TQDTSTLENP
660
AILDQLKETR
Length:660
Mass (Da):72,595
Last modified:September 1, 2009 - v1
Checksum:i5656BD9158B10654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001644 Genomic DNA. Translation: ACS63057.1.
RefSeqiWP_012762228.1. NC_012856.1.
YP_002981729.1. NC_012856.1.

Genome annotation databases

EnsemblBacteriaiACS63057; ACS63057; Rpic12D_1775.
KEGGirpf:Rpic12D_1775.
PATRICi20242597. VBIRalPic57998_2388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001644 Genomic DNA. Translation: ACS63057.1.
RefSeqiWP_012762228.1. NC_012856.1.
YP_002981729.1. NC_012856.1.

3D structure databases

ProteinModelPortaliC6BJD4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS63057; ACS63057; Rpic12D_1775.
KEGGirpf:Rpic12D_1775.
PATRICi20242597. VBIRalPic57998_2388.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiMRHTDCL.
OrthoDBiEOG68WR2H.

Enzyme and pathway databases

BioCyciRPIC428406:GH9Y-1808-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 12DImported.

Entry informationi

Entry nameiC6BJD4_RALP1
AccessioniPrimary (citable) accession number: C6BJD4
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.