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C6BJD4

- C6BJD4_RALP1

UniProt

C6BJD4 - C6BJD4_RALP1

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Ralstonia pickettii (strain 12D)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei317 – 3171Coenzyme AUniRule annotation
    Binding sitei397 – 3971Substrate; via nitrogen amideUniRule annotation
    Binding sitei512 – 5121SubstrateUniRule annotation
    Binding sitei528 – 5281SubstrateUniRule annotation
    Active sitei530 – 5301UniRule annotation
    Binding sitei536 – 5361Coenzyme AUniRule annotation
    Binding sitei539 – 5391SubstrateUniRule annotation
    Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei600 – 6001Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRPIC428406:GH9Y-1808-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:Rpic12D_1775Imported
    OrganismiRalstonia pickettii (strain 12D)Imported
    Taxonomic identifieri428406 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000008208: Chromosome 1

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei625 – 6251N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi428406.Rpic12D_1775.

    Structurei

    3D structure databases

    ProteinModelPortaliC6BJD4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiGGCEAVT.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6BJD4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGIESVLQE TRVFNPPESF VKQANIAGMD AYRALCAEAE KDYEGFWARL    50
    AHEHLLWHKP FSKVLDESNA PFYKWFEDGE LNASYNCLER NLENGNADKV 100
    AIIFETDDGN VSRITYRELH ARVCRFANGL KALGIKKGDR VVIYMPMSVE 150
    GIVAMQACAR IGATHSVVFG GFSAKSLQER IVDVGAVALI TADEQMRGGK 200
    ALPLKAIADE ALAMEGTGAV KHVIVYRRTN GNVNWVEGRD RAMDEVEAGQ 250
    PDTCEVTPVS AEHPLFILYT SGSTGKPKGV QHSTGGYLLW ALLTMQWTFD 300
    LKPDDIFWCT ADIGWVTGHS YIAYGPLAAG ATQVVFEGVP TYPNAGRFWD 350
    MIQRHKVNTF YTAPTAIRSL IKAAEADEKV HPKQYDLSSL RLLGTVGEPI 400
    NPEAWMWYHT NIGGGRCPIV DTFWQTETGG HMMTPLPGAT PLVPGSCTLP 450
    LPGIMAAVVD ETGHDVPNGQ GGILVVKRPW PSMIRTIWGD PERFKKSYFP 500
    EELGGKLYLA GDGSIRDKET GYFTIMGRID DVLNVSGHRM GTMEIESALV 550
    ANPIVAEAAV VGRPDDMTGE AICAFVVLKR ARPDGDEAKQ IANELRNWVG 600
    KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEDI TQDTSTLENP 650
    AILDQLKETR 660
    Length:660
    Mass (Da):72,595
    Last modified:September 1, 2009 - v1
    Checksum:i5656BD9158B10654
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001644 Genomic DNA. Translation: ACS63057.1.
    RefSeqiYP_002981729.1. NC_012856.1.

    Genome annotation databases

    EnsemblBacteriaiACS63057; ACS63057; Rpic12D_1775.
    GeneIDi8019431.
    KEGGirpf:Rpic12D_1775.
    PATRICi20242597. VBIRalPic57998_2388.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001644 Genomic DNA. Translation: ACS63057.1 .
    RefSeqi YP_002981729.1. NC_012856.1.

    3D structure databases

    ProteinModelPortali C6BJD4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 428406.Rpic12D_1775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS63057 ; ACS63057 ; Rpic12D_1775 .
    GeneIDi 8019431.
    KEGGi rpf:Rpic12D_1775.
    PATRICi 20242597. VBIRalPic57998_2388.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi GGCEAVT.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci RPIC428406:GH9Y-1808-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 12DImported.

    Entry informationi

    Entry nameiC6BJD4_RALP1
    AccessioniPrimary (citable) accession number: C6BJD4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3