C6BIT5 (C6BIT5_RALP1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Rpic12D_1574

Organism

Ralstonia pickettii (strain 12D) [Complete proteome] [HAMAP]

Taxonomic identifier

428406 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ACS62858.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	29	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	170	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	226	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	C6BIT5 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 5831A945B9C64F20	FASTA	252	26,559
	10 20 30 40 50 60 MSISHPGRRR FALIPSAGTG SRAGGDLPKQ YQAIAGRPML WHTAQAFLAS PEIDSVLVVT 70 80 90 100 110 120 TPGAQPLVQR FPDAGFNAAR LVEVDCGGDS RHASVTQGLA ALRRLGARDD DWVLVHDAAR 130 140 150 160 170 180 PGLTPALIAR LVAAVEAEGT QAAGGILALP VADTLKRADA AQHIVATLPR DGLWQAQTPQ 190 200 210 220 230 240 MFPLGLLERA LREALLQHRI VTDEASAIEA AGYPPLLVPG ALRNFKVTYP DDFALAEAIL 250 AQSAPPDEAS QA

References

[1]

"Complete sequence chromosome 1 of Ralstonia pickettii 12D." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Marsh T., Richardson P. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12D.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001644 Genomic DNA. Translation: ACS62858.1.
RefSeq	YP_002981530.1. NC_012856.1.
3D structure databases
ProteinModelPortal	C6BIT5.
ModBase	Search...
Protein-protein interaction databases
STRING	C6BIT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8019222.
GenomeReviews	Gene locus Rpic12D_1574 in contig CP001644_GR.
KEGG	rpf:Rpic12D_1574.
PATRIC	20242175. VBIRalPic57998_2185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SKVIVVC.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6BIT5_RALP1

Accession

Primary (citable) accession number: C6BIT5

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)