C6BBH3 (C6BBH3_RALP1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	Rpic12D_0278

Organism

Ralstonia pickettii (strain 12D) [Complete proteome] [HAMAP]

Taxonomic identifier

428406 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL ACS61585.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	99 – 109	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	16	1	By similarity HAMAP MF_00061
	Active site	141	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	C6BBH3 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: FEE03410156DAC3B	FASTA	289	31,358
	10 20 30 40 50 60 MSSAPVELRD CPAPAKLNLF LHVVGRRPDG YHLLQTVFQL IDWSDTLHFA RRPDGRLVRT 70 80 90 100 110 120 TDIPGVHADD DLVIRAARLL QAETGCTYGV DIAIEKRLPM GGGLGGGSSD AATTLLALNR 130 140 150 160 170 180 LWGLDLPRTQ LMAIGLKLGA DVPFFVFGQN AFAEGIGEEL TPVTLPPAAF VVIHPRVHVP 190 200 210 220 230 240 TPAIFSDEGL TRDTPLTIIT DFPDQQIVFA YGRNDLQAVA ERKYGEIARA LVWLRQFSPL 250 260 270 280 ARMTGSGACV FAPFDNVEQA QAVADQVPSE WEGRCAAGLS QHPLARFAV

References

[1]

"Complete sequence chromosome 1 of Ralstonia pickettii 12D." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N. , Ovchinnikova G., Marsh T., Richardson P. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 12D.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001644 Genomic DNA. Translation: ACS61585.1.
RefSeq	YP_002980257.1. NC_012856.1.
3D structure databases
ProteinModelPortal	C6BBH3.
ModBase	Search...
Protein-protein interaction databases
STRING	C6BBH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8017902.
GenomeReviews	Gene locus Rpic12D_0278 in contig CP001644_GR.
KEGG	rpf:Rpic12D_0278.
PATRIC	20239515. VBIRalPic57998_0877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

C6BBH3_RALP1

Accession

Primary (citable) accession number: C6BBH3

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)