ID   C6AZI8_RHILS            Unreviewed;       553 AA.
AC   C6AZI8;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   27-MAR-2024, entry version 80.
DE   RecName: Full=Alpha-amylase {ECO:0000256|RuleBase:RU361134};
DE            EC=3.2.1.1 {ECO:0000256|RuleBase:RU361134};
GN   OrderedLocusNames=Rleg_2115 {ECO:0000313|EMBL:ACS56392.1};
OS   Rhizobium leguminosarum bv. trifolii (strain WSM1325).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=395491 {ECO:0000313|EMBL:ACS56392.1, ECO:0000313|Proteomes:UP000002256};
RN   [1] {ECO:0000313|EMBL:ACS56392.1, ECO:0000313|Proteomes:UP000002256}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM1325 {ECO:0000313|EMBL:ACS56392.1,
RC   ECO:0000313|Proteomes:UP000002256};
RX   PubMed=21304718;
RA   Reeve W., O'Hara G., Chain P., Ardley J., Brau L., Nandesena K., Tiwari R.,
RA   Copeland A., Nolan M., Han C., Brettin T., Land M., Ovchinikova G.,
RA   Ivanova N., Mavromatis K., Markowitz V., Kyrpides N., Melino V., Denton M.,
RA   Yates R., Howieson J.;
RT   "Complete genome sequence of Rhizobium leguminosarum bv. trifolii strain
RT   WSM1325, an effective microsymbiont of annual Mediterranean clovers.";
RL   Stand. Genomic Sci. 2:347-356(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|RuleBase:RU361134};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|RuleBase:RU003615}.
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DR   EMBL; CP001622; ACS56392.1; -; Genomic_DNA.
DR   RefSeq; WP_012757608.1; NC_012850.1.
DR   AlphaFoldDB; C6AZI8; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; rlg:Rleg_2115; -.
DR   HOGENOM; CLU_006462_2_1_5; -.
DR   OrthoDB; 9805159at2; -.
DR   Proteomes; UP000002256; Chromosome.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd11334; AmyAc_TreS; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045857; O16G_dom_2.
DR   PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR10357:SF219; MALTOSE ALPHA-D-GLUCOSYLTRANSFERASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW   Glycosidase {ECO:0000256|RuleBase:RU361134};
KW   Hydrolase {ECO:0000256|RuleBase:RU361134}.
FT   DOMAIN          14..418
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   553 AA;  63452 MW;  0559A2D9D9D7D716 CRC64;
     MINDLWYKNA VIYCLSVETF MDANGDGVGD FQGLMRRLDY LSGLGVTAIW LMPFQASPGR
     DDGYDVSDYY NVDPRYGSLG DFVEFTHGAK QRGIRVLIDL VINHTSKDHP WFQDARSDPR
     SRYRDWYVWS ERKPDNADQG MVFPGVQKTT WTYDDRAKAY YFHRFYDHQP DLNTSNPEVQ
     AEILKIMGFW IQLGVSGFRM DAAPFIIATK GADVTKPVEQ FDMLRKFREF LQWRLGDSII
     LAEANILPKD NFEYFGDDGD RMQMMFNFQV NQALFYAFAS ADTRPLKKAM EATKPRPATA
     QWGLFLRNHD ELDLGRLTEK QRAAVFAAFG PEKDMQLYDR GIRRRLAPML GGDHRRIEMA
     YSLLFSLPGT PVIRYGDEIG MGDDLGLPER NCARTPMQWS TEPEGGFTKS EKPISPVIKD
     GPYGFQHVNV AEQRRDPNSL LNWTERMIRM RKEAPEIGWG DFSVIDTGDD GVLALRYDWR
     GNSVLILHNL HAQPAEVTFD PEIGEDGRQL IDIADGASSK ADEKGFHTVM LDAYGYRWYR
     VGGLDYLLRR TEI
//