C6A5B5 (NADE_THESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	TSIB_1759

Organism

Thermococcus sibiricus (strain MM 739 / DSM 12597) [Complete proteome] [HAMAP]

Taxonomic identifier

604354 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000204023
Regions
	Nucleotide binding	32 – 39	8	ATP By similarity
Sites
	Active site	34	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	C6A5B5 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: CD081F7A73FB48AF	FASTA	254	28,720
	10 20 30 40 50 60 MRSLDYAKVI TKLVSFIQEK VEESNVKGVI LGISGGVDSA TVAYLAARAI GKEKVLGLVM 70 80 90 100 110 120 PYHINRDVED ALLVCNNLGI NYKVINIKSI VNEFEKNLDF ELNNVSRGNI MSRTRMILLY 130 140 150 160 170 180 AHANSKNYLV LGTSNRSEFL TGYFTKWGDS ASDYAPLINL YKTEVWNIAR ILGVPNEIIN 190 200 210 220 230 240 KKPSAGLWEG QTDEKDLGIT YKLLDEILYR LVDLKMKKEN IARELNIPLN KVEYVESLIK 250 KSEHKRKLPI GPEI

References

[1]

"Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis." Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G. Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed: 19447963] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MM 739 / DSM 12597.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001463 Genomic DNA. Translation: ACS90810.1.
RefSeq	YP_002995159.1. NC_012883.1.
3D structure databases
ProteinModelPortal	C6A5B5.
ModBase	Search...
Protein-protein interaction databases
STRING	C6A5B5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8096769.
GenomeReviews	Gene locus TSIB_1759 in contig CP001463_GR.
KEGG	tsi:TSIB_1759.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YDISARD.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_THESM

Accession

Primary (citable) accession number: C6A5B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	September 1, 2009
Last modified:	November 16, 2011
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program