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C6A4V2 (C6A4V2_THESM) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase HAMAP-Rule MF_01133

Short name=RuBisCO HAMAP-Rule MF_01133
EC=4.1.1.39 HAMAP-Rule MF_01133
Gene names
Name:rbcL HAMAP-Rule MF_01133
Ordered Locus Names:TSIB_1596 EMBL ACS90647.1
OrganismThermococcus sibiricus (strain MM 739 / DSM 12597) [Complete proteome] [HAMAP] EMBL ACS90647.1
Taxonomic identifier604354 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase By similarity. HAMAP-Rule MF_01133

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01133

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01133

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01133

Subunit structure

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits By similarity. HAMAP-Rule MF_01133

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains (PubMed:17303759) By similarity. HAMAP-Rule MF_01133

Sequence similarities

Belongs to the RuBisCO large chain family. Type III subfamily. HAMAP-Rule MF_01133

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region370 – 3723Substrate binding By similarity HAMAP-Rule MF_01133
Region392 – 3954Substrate binding By similarity HAMAP-Rule MF_01133

Sites

Active site1661Proton acceptor By similarity HAMAP-Rule MF_01133
Active site2841Proton acceptor By similarity HAMAP-Rule MF_01133
Metal binding1921Magnesium; via carbamate group By similarity HAMAP-Rule MF_01133
Metal binding1941Magnesium By similarity HAMAP-Rule MF_01133
Metal binding1951Magnesium By similarity HAMAP-Rule MF_01133
Binding site1681Substrate By similarity HAMAP-Rule MF_01133
Binding site2851Substrate By similarity HAMAP-Rule MF_01133
Binding site3171Substrate By similarity HAMAP-Rule MF_01133
Site3251Transition state stabilizer By similarity HAMAP-Rule MF_01133

Amino acid modifications

Modified residue1921N6-carboxylysine By similarity HAMAP-Rule MF_01133

Sequences

Sequence LengthMass (Da)Tools
C6A4V2 [UniParc].

Last modified September 1, 2009. Version 1.
Checksum: 3864194B64ADDBB5

FASTA44750,441
        10         20         30         40         50         60 
MVKMPEKFDK IYDYYVDKNY EPNFKRDIVA VFRITPTDGY TIEQAAGAVA AESSTGTWTT 

        70         80         90        100        110        120 
LYPWYEEERW SDLSAKAYYF HDMGDGSWIV RVAYPAHAFE EWNLPGLLAS IAGNVFGMKR 

       130        140        150        160        170        180 
VKGLRLEDLY LPEIVLRDFS GPNKGIKGVR ETLEIKDRPI YGVVPKPKVG YSPEELEKLS 

       190        200        210        220        230        240 
YELLSAGADY IKDDENLTGP WYNRFEERAD VMARVIDRVE AETGEKKTWF ANITADIREM 

       250        260        270        280        290        300 
ENRLEILAEY GLPHAMVDVV IVGWASLEYI RDLAEDYDIA LHAHRAMHAT FTRNHYHGIS 

       310        320        330        340        350        360 
MFVLAKLYRI IGLDQLHVGT AGAGKLEGEK WDVIQNARIL REEHYKPDKN DVFHLDQKFY 

       370        380        390        400        410        420 
HIKPAFPTSS GGLHPGNLPQ VINALGSDIV LQLGGGTLGH PDGPAAGAKA VRQALDAIMQ 

       430        440 
GISLDEYAKT HKELARALEK WGHVTPV 

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References

[1]"Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MM 739 / DSM 12597.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001463 Genomic DNA. Translation: ACS90647.1.
RefSeqYP_002994996.1. NC_012883.1.

3D structure databases

ProteinModelPortalC6A4V2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING604354.TSIB_1596.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS90647; ACS90647; TSIB_1596.
GeneID8096606.
KEGGtsi:TSIB_1596.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMAHRAMHAA.
ProtClustDBPRK04208.

Enzyme and pathway databases

BioCycTSIB604354:GHMS-1639-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01133. RuBisCO_L_type3.
InterProIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsTIGR03326. rubisco_III. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC6A4V2_THESM
AccessionPrimary (citable) accession number: C6A4V2
Entry history
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)