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C6A4V2

- C6A4V2_THESM

UniProt

C6A4V2 - C6A4V2_THESM

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661Proton acceptorUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Metal bindingi192 – 1921Magnesium; via carbamate groupUniRule annotation
    Metal bindingi194 – 1941MagnesiumUniRule annotation
    Metal bindingi195 – 1951MagnesiumUniRule annotation
    Active sitei284 – 2841Proton acceptorUniRule annotation
    Binding sitei285 – 2851SubstrateUniRule annotation
    Binding sitei317 – 3171SubstrateUniRule annotation
    Sitei325 – 3251Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciTSIB604354:GHMS-1639-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:TSIB_1596Imported
    OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)Imported
    Taxonomic identifieri604354 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000009079: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi604354.TSIB_1596.

    Structurei

    3D structure databases

    ProteinModelPortaliC6A4V2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni370 – 3723Substrate bindingUniRule annotation
    Regioni392 – 3954Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C6A4V2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKMPEKFDK IYDYYVDKNY EPNFKRDIVA VFRITPTDGY TIEQAAGAVA    50
    AESSTGTWTT LYPWYEEERW SDLSAKAYYF HDMGDGSWIV RVAYPAHAFE 100
    EWNLPGLLAS IAGNVFGMKR VKGLRLEDLY LPEIVLRDFS GPNKGIKGVR 150
    ETLEIKDRPI YGVVPKPKVG YSPEELEKLS YELLSAGADY IKDDENLTGP 200
    WYNRFEERAD VMARVIDRVE AETGEKKTWF ANITADIREM ENRLEILAEY 250
    GLPHAMVDVV IVGWASLEYI RDLAEDYDIA LHAHRAMHAT FTRNHYHGIS 300
    MFVLAKLYRI IGLDQLHVGT AGAGKLEGEK WDVIQNARIL REEHYKPDKN 350
    DVFHLDQKFY HIKPAFPTSS GGLHPGNLPQ VINALGSDIV LQLGGGTLGH 400
    PDGPAAGAKA VRQALDAIMQ GISLDEYAKT HKELARALEK WGHVTPV 447
    Length:447
    Mass (Da):50,441
    Last modified:September 1, 2009 - v1
    Checksum:i3864194B64ADDBB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001463 Genomic DNA. Translation: ACS90647.1.
    RefSeqiYP_002994996.1. NC_012883.1.

    Genome annotation databases

    EnsemblBacteriaiACS90647; ACS90647; TSIB_1596.
    GeneIDi8096606.
    KEGGitsi:TSIB_1596.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001463 Genomic DNA. Translation: ACS90647.1 .
    RefSeqi YP_002994996.1. NC_012883.1.

    3D structure databases

    ProteinModelPortali C6A4V2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 604354.TSIB_1596.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS90647 ; ACS90647 ; TSIB_1596 .
    GeneIDi 8096606.
    KEGGi tsi:TSIB_1596.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci TSIB604354:GHMS-1639-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
      Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
      Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MM 739 / DSM 12597Imported.

    Entry informationi

    Entry nameiC6A4V2_THESM
    AccessioniPrimary (citable) accession number: C6A4V2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3