C6A4U4 (C6A4U4_THESM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	TSIB_1588

Organism

Thermococcus sibiricus (strain MM 739 / DSM 12597) [Complete proteome] [HAMAP]

Taxonomic identifier

604354 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	1442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C6A4U4 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 9DBC581D88141A21	FASTA	1,442	164,327
	10 20 30 40 50 60 MEIYSEEMKK YFEFLQREID KAYEIAEKAR SQGKDPTQEI EVPQATDMAG RVESLVGPKG 70 80 90 100 110 120 VAERIRSLVK EYGKELAALK VVDEIIEGKF KKFENKEQLA DQCVRTALAI LTEGIVSAPL 130 140 150 160 170 180 EGIADVKIKK NEDGTNYLAV YYAGPIRSSG GTAQALSVLV GDYVRKKLGL DRFKLTEEHV 190 200 210 220 230 240 ERYVEEIDLY HRAVTRLQYY PSPDEVRLAV RNIPIEITGE TTDNVEVSHR NVPGVETNYL 250 260 270 280 290 300 RGGAILVIAE GVLQKANKLV KYIDKMGIDG WEWLKEFVEA KEKGKSDEKE EKALEESKTE 310 320 330 340 350 360 TAVGETTKIK KGFYHELYEK LKATISPNAK YTKEIIGGRP LFAEPSTNGG FRLRYGRSRV 370 380 390 400 410 420 SGFATWSINP AVMIMVDEFL AVGTQMKTER PGKGCIVTPA STIEGPIVKL KDGSVVRVDD 430 440 450 460 470 480 YHKALELKDR VEEILYLGDA LIAFGDFVEN NQTLLPANYV EEWWIQELVE TVNDVYELEL 490 500 510 520 530 540 RPFQENERDS VEEAADYLDI EPETLAKLLY DPLVTKPTIE EALHLSNILK IPLHPYYTLY 550 560 570 580 590 600 WNTLTVEELI KLQNALVNAT IEWGEYRGFR YAKRLEISLE TLGGAKRYLE LLGVEHIIEG 610 620 630 640 650 660 DTVIVDYPWA AAVLTPLANL EKKIEFKEFH TPIDLINEIS DIKLRDRGIS WIGSRMGRPE 670 680 690 700 710 720 KAKERKMKPP VHVLFPIGLA GGNSRDIYKA AQEGKTSDVE IAHFKCTSCG HVDIFPTCPE 730 740 750 760 770 780 CGEEAKLLYH CPKCGFESIL DTRCPKCDIE MKPYKRRRIS PSELLNSAMQ NVGIYTLDKL 790 800 810 820 830 840 KGVMGMSSAH KIAEPLEKGI LRARNDVYVF KDGTIRFDAT DAPITHFKPK EIGVSIEKLK 850 860 870 880 890 900 ELGYTHDFEG KPLVSGDQIL ELKVQDIILS KEAGRYLVKV AKFVDDLLDK VYGLPRFYNV 910 920 930 940 950 960 EKMEDLIGHL VIGLAPHTSA GIIGRIIGFV DALVGYAHPY YHAAKRRNCF PGETRILVQI 970 980 990 1000 1010 1020 DGFPQRITLK ELYELFEDEH YENMVYVRKK PKADIKVYSF DPETGKVVLT DIEDVIKAPI 1030 1040 1050 1060 1070 1080 TDHLIRFELE LGRSFETTID HPVLVYENGK FFKKRAFEVK ESDIMVVIDE SDSKPLKITI 1090 1100 1110 1120 1130 1140 KKIEFVKPTG DFVFSLNAKN YHNVLINENI VTHQCDGDED SVMLLLDALL NFSRYYLPEK 1150 1160 1170 1180 1190 1200 RGGKMDAPLV VTTRLDPREV DSEVHNMDVV RYYPLEFYRA TYELKSPKEV VGVIERVEDR 1210 1220 1230 1240 1250 1260 LGKPEMYEGL KFTHDTDDIA LGPKMSLYKQ LGDMVDKVSH QLALAERIRA VNEHHVAETI 1270 1280 1290 1300 1310 1320 INSHIVPDLR GNLRSFTRQE FRCVKCNTKY RRPPLRGKCT KCGGKIVLTV SKGAIEKYLP 1330 1340 1350 1360 1370 1380 TAKMLVAKYN VIDYTRQRIC VTEKDIKSLF QNVFPETQRT LLALNHRDIC DRMIAERTGR 1390 1400 1410 1420 1430 1440 TVKGNGYLDE LKENGKWKKT EKKQSSVKER QIKRKKGEKE EIQKPKKKKQ SKKVINLEDF FS

References

[1]

"Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis." Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G. Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed: 19447963] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001463 Genomic DNA. Translation: ACS90639.1.
RefSeq	YP_002994988.1. NC_012883.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C6A4U4.
Protein family/group databases
MEROPS	N10.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8096598.
GenomeReviews	Gene locus TSIB_1588 in contig CP001463_GR.
KEGG	tsi:TSIB_1588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NCDGDED.
ProtClustDB	PRK14714.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01445. Intein_Nterm. 1 hit. TIGR00354. PolC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C6A4U4_THESM

Accession

Primary (citable) accession number: C6A4U4

Entry history

Integrated into UniProtKB/TrEMBL:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	November 16, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)