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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPUniRule annotation1
Active sitei314Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 294ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:G1GV3-1497-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:TSIB_1528
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000009079 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002040991 – 350RNA 3'-terminal phosphate cyclaseAdd BLAST350

Proteomic databases

PRIDEiC6A4N4

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_1528

Structurei

3D structure databases

SMRiC6A4N4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
HOGENOMiHOG000015264
KOiK18105
OMAiANRPKPG
OrthoDBiPOG093Z05RD

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

C6A4N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVIDGSYGE GGGQILRTAV ALSVITGEPI KIINIRAKRS NPGLRPQHLH
60 70 80 90 100
GILALKELSD AKVKGAKEGS TELEFYPKST RVRHVKVLIK TAGSISLVLQ
110 120 130 140 150
ALLPAMVFAE EEVTFEITGG TDVAWSPPVD YLKHITLYAL EKLGIKVEIE
160 170 180 190 200
IRRRGHYPRG GGFVIGKVYP WGTKRPLVAR TFDKIYSFEG ISHAVRLPSH
210 220 230 240 250
VAIRQAKAAK EALERVYPSI PIKIHEEYYE QGKDPHFGPG SGIVIWANTD
260 270 280 290 300
VLRLGGDALG ERGKPAEIVG REAAKALIEQ LGPRHAVDKF LGDQLIPFLT
310 320 330 340 350
FAGGDIWVSE VTKHLITNVW VVEQFFGRVF EMEGEIGKPG KVRVVKKVEL
Length:350
Mass (Da):38,601
Last modified:September 1, 2009 - v1
Checksum:i53A3AE817C034A5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA Translation: ACS90579.1
RefSeqiWP_015849796.1, NC_012883.1

Genome annotation databases

EnsemblBacteriaiACS90579; ACS90579; TSIB_1528
GeneIDi8096536
KEGGitsi:TSIB_1528

Similar proteinsi

Entry informationi

Entry nameiRTCA_THESM
AccessioniPrimary (citable) accession number: C6A4N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: March 28, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health