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Protein

DNA ligase

Gene

lig

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation
NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248ATPUniRule annotation1
Active sitei250N6-AMP-lysine intermediateUniRule annotation1
Binding sitei255ATPUniRule annotation1
Binding sitei270AMPCombined sources1
Binding sitei270ATPUniRule annotation1
Binding sitei300AMPCombined sources1
Binding sitei300ATPUniRule annotation1
Binding sitei340AMPCombined sources1
Binding sitei340ATPUniRule annotation1
Binding sitei404AMPCombined sources1
Binding sitei415AMPCombined sources1
Binding sitei415ATPUniRule annotation1
Binding sitei421ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 251AMPCombined sources4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processCell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:G1GV3-870-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.6UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:TSIB_0885Imported
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)Imported
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000009079 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_0885

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EQ5X-ray2.85A2-560[»]
ProteinModelPortaliC6A2U9
SMRiC6A2U9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini328 – 456DNA_LIGASE_A3InterPro annotationAdd BLAST129

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347 Archaea
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG093Z03L0

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

C6A2U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYKELVELY KRLEKTTLKT LKTKFVSDFL KSVEKPELLE VIPYLILGKV
60 70 80 90 100
FPDWDERELG IGEKLLIKAV SMATGINSEE IENSVRDTGD LGESIALALN
110 120 130 140 150
KRKQKSFFSQ PLTIERVYNT LVKIAEASGA GSQDRKLKYL ANLFMDASPD
160 170 180 190 200
EGKYLARTVL GIMRTGVAEG LLRDALADAF KVRVELVERA YMLTSDFGFV
210 220 230 240 250
AKVAKLEGDE GLAKVKIQVG KPIKPMLAQM AANVREALVE MGGEAEFEIK
260 270 280 290 300
YDGARVQVHK NGNKVLIYSR RLENVTKSIP EVVERVKEAL KPEKVIVEGE
310 320 330 340 350
LVAVEETGRP RPFQYVLRRF RRKYNIEEMI EKIPLELNLF DILYVDGQNM
360 370 380 390 400
IDTPFMERRK VLESVVNSNE WIKSAENLIT KSPEEAEAFY HKALDLGHEG
410 420 430 440 450
LMAKRLDSTY EPGNRGKKWL KIKPTMENLD LVVLGAEWGE GRRSGVLSSF
460 470 480 490 500
LLGAYDPVKG DFVPVGKVGS GFTDEDLVEF TKMLKPLIKK EHGKEVELEP
510 520 530 540 550
KVVIEVAYQE IQKSPKYESG FALRFPRYIA LREDKGPEDA DTVQRLAELY
560
QFQERLKGGR
Length:560
Mass (Da):63,448
Last modified:September 1, 2009 - v1
Checksum:i3B20F1C68A04EDA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA Translation: ACS89944.1
RefSeqiWP_015849163.1, NC_012883.1

Genome annotation databases

EnsemblBacteriaiACS89944; ACS89944; TSIB_0885
GeneIDi8095876
KEGGitsi:TSIB_0885

Similar proteinsi

Entry informationi

Entry nameiC6A2U9_THESM
AccessioniPrimary (citable) accession number: C6A2U9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health