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Protein

DNA ligase

Gene

lig

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation
NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248ATPUniRule annotation1
Active sitei250N6-AMP-lysine intermediateUniRule annotation1
Binding sitei255ATPUniRule annotation1
Binding sitei270AMPCombined sources1
Binding sitei270ATPUniRule annotation1
Binding sitei300AMPCombined sources1
Binding sitei300ATPUniRule annotation1
Binding sitei340AMPCombined sources1
Binding sitei340ATPUniRule annotation1
Binding sitei404AMPCombined sources1
Binding sitei415AMPCombined sources1
Binding sitei415ATPUniRule annotation1
Binding sitei421ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 251AMPCombined sources4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.6UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP/NAD(+)]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:TSIB_0885Imported
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)Imported
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000009079 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_0885.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EQ5X-ray2.85A2-560[»]
ProteinModelPortaliC6A2U9.
SMRiC6A2U9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini328 – 456DNA_LIGASE_A3InterPro annotationAdd BLAST129

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiDVETLDC.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6A2U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYKELVELY KRLEKTTLKT LKTKFVSDFL KSVEKPELLE VIPYLILGKV
60 70 80 90 100
FPDWDERELG IGEKLLIKAV SMATGINSEE IENSVRDTGD LGESIALALN
110 120 130 140 150
KRKQKSFFSQ PLTIERVYNT LVKIAEASGA GSQDRKLKYL ANLFMDASPD
160 170 180 190 200
EGKYLARTVL GIMRTGVAEG LLRDALADAF KVRVELVERA YMLTSDFGFV
210 220 230 240 250
AKVAKLEGDE GLAKVKIQVG KPIKPMLAQM AANVREALVE MGGEAEFEIK
260 270 280 290 300
YDGARVQVHK NGNKVLIYSR RLENVTKSIP EVVERVKEAL KPEKVIVEGE
310 320 330 340 350
LVAVEETGRP RPFQYVLRRF RRKYNIEEMI EKIPLELNLF DILYVDGQNM
360 370 380 390 400
IDTPFMERRK VLESVVNSNE WIKSAENLIT KSPEEAEAFY HKALDLGHEG
410 420 430 440 450
LMAKRLDSTY EPGNRGKKWL KIKPTMENLD LVVLGAEWGE GRRSGVLSSF
460 470 480 490 500
LLGAYDPVKG DFVPVGKVGS GFTDEDLVEF TKMLKPLIKK EHGKEVELEP
510 520 530 540 550
KVVIEVAYQE IQKSPKYESG FALRFPRYIA LREDKGPEDA DTVQRLAELY
560
QFQERLKGGR
Length:560
Mass (Da):63,448
Last modified:September 1, 2009 - v1
Checksum:i3B20F1C68A04EDA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89944.1.
RefSeqiWP_015849163.1. NC_012883.1.

Genome annotation databases

EnsemblBacteriaiACS89944; ACS89944; TSIB_0885.
GeneIDi8095876.
KEGGitsi:TSIB_0885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89944.1.
RefSeqiWP_015849163.1. NC_012883.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EQ5X-ray2.85A2-560[»]
ProteinModelPortaliC6A2U9.
SMRiC6A2U9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi604354.TSIB_0885.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS89944; ACS89944; TSIB_0885.
GeneIDi8095876.
KEGGitsi:TSIB_0885.

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiDVETLDC.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC6A2U9_THESM
AccessioniPrimary (citable) accession number: C6A2U9
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.