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Protein

Ribonuclease Z

Gene

rnz

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Zinc 1; catalyticUniRule annotation1
Metal bindingi63Zinc 1; catalyticUniRule annotation1
Active sitei65Proton acceptorUniRule annotation1
Metal bindingi65Zinc 2; catalyticUniRule annotation1
Metal bindingi66Zinc 2; catalyticUniRule annotation1
Metal bindingi137Zinc 1; catalyticUniRule annotation1
Metal bindingi207Zinc 1; catalyticUniRule annotation1
Metal bindingi207Zinc 2; catalyticUniRule annotation1
Metal bindingi263Zinc 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processtRNA processing
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation)
Short name:
RNase ZUniRule annotation
Alternative name(s):
tRNA 3 endonucleaseUniRule annotation
tRNase ZUniRule annotation
Gene namesi
Name:rnzUniRule annotation
Ordered Locus Names:TSIB_0827
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000009079 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002160211 – 306Ribonuclease ZAdd BLAST306

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi604354.TSIB_0827.

Structurei

3D structure databases

SMRiC6A2P3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase Z family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.
OrthoDBiPOG093Z04VY.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiView protein in InterPro
IPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
PfamiView protein in Pfam
PF12706. Lactamase_B_2. 2 hits.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

C6A2P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEVIFLGTG GIMPTKERNV PAIALKYGGE IILWDIGEGT LRQLDTTKLS
60 70 80 90 100
PMKIEKIFIT HFHGDHYLGL MSLIQTMTLW KRERPLYIYG PKYTFEFIQN
110 120 130 140 150
YLKSGFFRPS FEIHIHELGE TRLKFKDYEI WSFKVDHGIP ALGYVFKEND
160 170 180 190 200
KRGNFDVEKI RELGLKPGPW MKKLEIKGKI EINGKLIYLE DVTGEPKKGV
210 220 230 240 250
KIVYTGDTEQ CERIKLFSER ADLLIHEATY LSREDKGDSY HSTVEEACET
260 270 280 290 300
AKRAKVELLA LFHRAPRYKY EEYVQEANKI CEYKFIIPQD FDVVRLGEKY

ELSSLR
Length:306
Mass (Da):35,764
Last modified:September 1, 2009 - v1
Checksum:i6E7529E1F8C3EB82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89888.1.
RefSeqiWP_015849108.1. NC_012883.1.

Genome annotation databases

EnsemblBacteriaiACS89888; ACS89888; TSIB_0827.
GeneIDi8095818.
KEGGitsi:TSIB_0827.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89888.1.
RefSeqiWP_015849108.1. NC_012883.1.

3D structure databases

SMRiC6A2P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi604354.TSIB_0827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS89888; ACS89888; TSIB_0827.
GeneIDi8095818.
KEGGitsi:TSIB_0827.

Phylogenomic databases

eggNOGiarCOG00501. Archaea.
COG1234. LUCA.
HOGENOMiHOG000272419.
KOiK00784.
OMAiSYAFCSD.
OrthoDBiPOG093Z04VY.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01818. RNase_Z_BN. 1 hit.
InterProiView protein in InterPro
IPR001279. Metallo-B-lactamas.
IPR013471. RNase_Z/BN.
PfamiView protein in Pfam
PF12706. Lactamase_B_2. 2 hits.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
TIGRFAMsiTIGR02651. RNase_Z. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNZ_THESM
AccessioniPrimary (citable) accession number: C6A2P3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: March 15, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.