Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C6A0S4

- PCKG_THESM

UniProt

C6A0S4 - PCKG_THESM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861SubstrateUniRule annotation
Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
Metal bindingi229 – 2291ManganeseUniRule annotation
Binding sitei229 – 2291SubstrateUniRule annotation
Metal bindingi248 – 2481Manganese; via tele nitrogenUniRule annotation
Binding sitei270 – 2701SubstrateUniRule annotation
Active sitei272 – 2721UniRule annotation
Metal bindingi289 – 2891ManganeseUniRule annotation
Binding sitei386 – 3861GTPUniRule annotation
Binding sitei418 – 4181GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:GHMS-156-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:TSIB_0151
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000009079: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_1000206234Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_0151.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6A0S4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK
60 70 80 90 100
EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE
110 120 130 140 150
IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID
210 220 230 240 250
KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA
310 320 330 340 350
RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE
360 370 380 390 400
MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE
410 420 430 440 450
ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL
510 520 530 540 550
REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK
560 570 580 590 600
EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE
610 620
ERHRLIEAKE KYGDYISPFK FL
Length:622
Mass (Da):71,790
Last modified:September 1, 2009 - v1
Checksum:i68A4AFFA3E5792FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001463 Genomic DNA. Translation: ACS89219.1.
RefSeqiYP_002993568.1. NC_012883.1.

Genome annotation databases

EnsemblBacteriaiACS89219; ACS89219; TSIB_0151.
GeneIDi8095123.
KEGGitsi:TSIB_0151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001463 Genomic DNA. Translation: ACS89219.1 .
RefSeqi YP_002993568.1. NC_012883.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 604354.TSIB_0151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACS89219 ; ACS89219 ; TSIB_0151 .
GeneIDi 8095123.
KEGGi tsi:TSIB_0151.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci TSIB604354:GHMS-156-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
    Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
    Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MM 739 / DSM 12597.

Entry informationi

Entry nameiPCKG_THESM
AccessioniPrimary (citable) accession number: C6A0S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3