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C6A0S4

- PCKG_THESM

UniProt

C6A0S4 - PCKG_THESM

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861SubstrateUniRule annotation
    Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
    Metal bindingi229 – 2291ManganeseUniRule annotation
    Binding sitei229 – 2291SubstrateUniRule annotation
    Metal bindingi248 – 2481Manganese; via tele nitrogenUniRule annotation
    Binding sitei270 – 2701SubstrateUniRule annotation
    Active sitei272 – 2721UniRule annotation
    Metal bindingi289 – 2891ManganeseUniRule annotation
    Binding sitei386 – 3861GTPUniRule annotation
    Binding sitei418 – 4181GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTSIB604354:GHMS-156-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:TSIB_0151
    OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
    Taxonomic identifieri604354 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000009079: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_1000206234Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi604354.TSIB_0151.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni384 – 3863Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C6A0S4-1 [UniParc]FASTAAdd to Basket

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    MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK    50
    EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE 100
    IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL 150
    TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID 200
    KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF 250
    LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA 300
    RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE 350
    MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE 400
    ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA 450
    TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL 500
    REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK 550
    EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE 600
    ERHRLIEAKE KYGDYISPFK FL 622
    Length:622
    Mass (Da):71,790
    Last modified:September 1, 2009 - v1
    Checksum:i68A4AFFA3E5792FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001463 Genomic DNA. Translation: ACS89219.1.
    RefSeqiWP_012766180.1. NC_012883.1.
    YP_002993568.1. NC_012883.1.

    Genome annotation databases

    EnsemblBacteriaiACS89219; ACS89219; TSIB_0151.
    GeneIDi8095123.
    KEGGitsi:TSIB_0151.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001463 Genomic DNA. Translation: ACS89219.1 .
    RefSeqi WP_012766180.1. NC_012883.1.
    YP_002993568.1. NC_012883.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 604354.TSIB_0151.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS89219 ; ACS89219 ; TSIB_0151 .
    GeneIDi 8095123.
    KEGGi tsi:TSIB_0151.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci TSIB604354:GHMS-156-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
      Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
      Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MM 739 / DSM 12597.

    Entry informationi

    Entry nameiPCKG_THESM
    AccessioniPrimary (citable) accession number: C6A0S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3