Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861SubstrateUniRule annotation
Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
Metal bindingi229 – 2291ManganeseUniRule annotation
Binding sitei229 – 2291SubstrateUniRule annotation
Metal bindingi248 – 2481Manganese; via tele nitrogenUniRule annotation
Binding sitei270 – 2701SubstrateUniRule annotation
Active sitei272 – 2721UniRule annotation
Metal bindingi289 – 2891ManganeseUniRule annotation
Binding sitei386 – 3861GTPUniRule annotation
Binding sitei418 – 4181GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:GHMS-156-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:TSIB_0151
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000009079 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_1000206234Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_0151.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni384 – 3863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6A0S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK
60 70 80 90 100
EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE
110 120 130 140 150
IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID
210 220 230 240 250
KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA
310 320 330 340 350
RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE
360 370 380 390 400
MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE
410 420 430 440 450
ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL
510 520 530 540 550
REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK
560 570 580 590 600
EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE
610 620
ERHRLIEAKE KYGDYISPFK FL
Length:622
Mass (Da):71,790
Last modified:September 1, 2009 - v1
Checksum:i68A4AFFA3E5792FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89219.1.
RefSeqiWP_012766180.1. NC_012883.1.
YP_002993568.1. NC_012883.1.

Genome annotation databases

EnsemblBacteriaiACS89219; ACS89219; TSIB_0151.
GeneIDi8095123.
KEGGitsi:TSIB_0151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS89219.1.
RefSeqiWP_012766180.1. NC_012883.1.
YP_002993568.1. NC_012883.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi604354.TSIB_0151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS89219; ACS89219; TSIB_0151.
GeneIDi8095123.
KEGGitsi:TSIB_0151.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciTSIB604354:GHMS-156-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
    Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
    Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MM 739 / DSM 12597.

Entry informationi

Entry nameiPCKG_THESM
AccessioniPrimary (citable) accession number: C6A0S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: May 27, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.