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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:GHMS-2010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:TSIB_1964
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
ProteomesiUP000009079: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Tryptophan--tRNA ligasePRO_1000203257Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_1964.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 899"HIGH" region
Motifi252 – 2565"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224742.
KOiK01867.
OMAiKPETAIY.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_A. Trp_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C6A032-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEFEVTPWE VTGVVDYNKL IEEFGTTPLT DDLLKKTEEL TKKELPMYFK
60 70 80 90 100
RKFFFSHRDY DLVLKDYEAG KGFFLYTGRG PSGPMHIGHI IPFFATKWLQ
110 120 130 140 150
ENFGVNLYVQ ITDDEKFLFK PQLTFEGTKR WAYENILDII AVGFDPDKTF
160 170 180 190 200
IFQDSEFTKI YEMAIPIAKK VTYSMAKAVF GFNEQSKIGM IFFPAIQAAP
210 220 230 240 250
TFFEEKRSLI PAAIDQDPYW RIQRDFAESL GYYKTAALHS KFVPGLMGLG
260 270 280 290 300
GKMSASKPET AIYLTDDPEE AGKKIWKYAL TGGRATAKEQ RELGGEPDKC
310 320 330 340 350
VVFKWLEIFF EPDEKKLLER YIACKNGEIL CGQCKRYLIE KVQNFLKEHQ
360 370 380
EKREKAKKEI EKFKYTGDLA REQWDKAIPE PLRK
Length:384
Mass (Da):44,610
Last modified:September 1, 2009 - v1
Checksum:i5EE79B3DAC942C65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS91013.1.
RefSeqiYP_002995362.1. NC_012883.1.

Genome annotation databases

EnsemblBacteriaiACS91013; ACS91013; TSIB_1964.
GeneIDi8096977.
KEGGitsi:TSIB_1964.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA. Translation: ACS91013.1.
RefSeqiYP_002995362.1. NC_012883.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi604354.TSIB_1964.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS91013; ACS91013; TSIB_1964.
GeneIDi8096977.
KEGGitsi:TSIB_1964.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224742.
KOiK01867.
OMAiKPETAIY.

Enzyme and pathway databases

BioCyciTSIB604354:GHMS-2010-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_A. Trp_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR020653. Tryptophan-tRNA-ligase_arc.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis."
    Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.
    Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MM 739 / DSM 12597.

Entry informationi

Entry nameiSYW_THESM
AccessioniPrimary (citable) accession number: C6A032
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: February 4, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.