C5ZZZ3 (SYR_THESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	TSIB_1925

Organism

Thermococcus sibiricus (strain MM 739 / DSM 12597) [Complete proteome] [HAMAP]

Taxonomic identifier

604354 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus sibiricus

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000203108
Regions
	Motif	134 – 144	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	C5ZZZ3 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 2AEBBCFC4ED85354	FASTA	644	73,624
	10 20 30 40 50 60 MTYDDIKAKV GELLKTIISE MLEKEGKTWE GEILFDETPN MEFGDFATTV AFLLAKVFKK 70 80 90 100 110 120 APRIIAQEIA SNLEDKLPEY IAKVEVAGAG YINFFLDYEK FSKLTVKEIL QKGEHFGESK 130 140 150 160 170 180 IGEGKKVIVE HTSVNPTKPL HMGHARNAVL GDTVARIMRA LGYNVEVQNY IDDLGVQFAQ 190 200 210 220 230 240 VLWGYLNMRE EFESIIKELK EKGLSKEDIL DHALGLLYVE VHKKMEEYPE VEREIRSIMK 250 260 270 280 290 300 ELEKEDNEIS EKGRKLAEDV VKAQMKTTYR LSISYDLLSW ESDIVRSGIF EESYKRIQEN 310 320 330 340 350 360 SHFEWAQEGK YKGAFIMKLG DLFPDLENPD TVLIRSDGTA TYTGKDIAYH LWKFGKVSAD 370 380 390 400 410 420 MRYKVWDKIN DHKTWTTAKD GERMPGRFAN AEIVINVVGS EQRYEQMAVA YALKLLGYEE 430 440 450 460 470 480 EYHNFHHLAY EHVVRPEGKF SGRKGTWIGF TVDEVLDEAV KRAKELVEEK NPSLSGEEKE 490 500 510 520 530 540 KIAEIVGVGA VRYNLVKYSP EKIITFRWED VLNFEGESAP YIQYAHARCA SILKKAMEEG 550 560 570 580 590 600 INIDKDALLK NADFSKLDNK EKELIKIISK FPEIVKTAGR DIKPHLLATY ANELAMVFNS 610 620 630 640 FYMALPVLKA EEGVRELRLL LVIATKQVLK NTLGLMGIEA PEVM

References

[1]

"Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis." Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G. Appl. Environ. Microbiol. 75:4580-4588(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MM 739 / DSM 12597.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001463 Genomic DNA. Translation: ACS90974.1.
RefSeq	YP_002995323.1. NC_012883.1.
3D structure databases
ProteinModelPortal	C5ZZZ3.
ModBase	Search...
Protein-protein interaction databases
STRING	604354.TSIB_1925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS90974; ACS90974; TSIB_1925.
GeneID	8096937.
KEGG	tsi:TSIB_1925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247213.
KO	K01887.
OMA	VHPLHIG.
ProtClustDB	PRK01611.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 2 hits. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_THESM

Accession

Primary (citable) accession number: C5ZZZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program