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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (SORBI_001G071800), Glucose-6-phosphate isomerase (SORBI_002G230600)
  3. ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase (SORBI_003G393900), Fructose-bisphosphate aldolase (SORBI_003G096000), Fructose-bisphosphate aldolase (SORBI_004G146000), Fructose-bisphosphate aldolase (SORBI_008G053200), Fructose-bisphosphate aldolase (SORBI_005G056400)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125ATP; via amide nitrogenUniRule annotation1
Metal bindingi216Magnesium; catalyticUniRule annotation1
Sitei217Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei246Proton acceptorUniRule annotation1
Binding sitei345SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi190 – 191ATPUniRule annotation2
Nucleotide bindingi215 – 218ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKUniRule annotation
ORF Names:SORBI_006G114700Imported
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)Imported
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeSorghinaeSorghum
Proteomesi
  • UP000000768 Componenti: Chromosome 6

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi4558.Sb06g019610.1.

Structurei

3D structure databases

ProteinModelPortaliC5YAA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 427PFKInterPro annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni244 – 246Substrate bindingUniRule annotation3
Regioni289 – 291Substrate bindingUniRule annotation3
Regioni403 – 406Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiC5YAA9.
KOiK00850.
OrthoDBiEOG093608ZW.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

C5YAA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPTAARSSG GTPAAAENDA TAPTNTTVTL PPLTLRDVPR LPSALASAAS
60 70 80 90 100
PSPTPAVQNP ISRHPYFHPP ATFYISPGDV TLRHAFFDLA SAAPSPLVAY
110 120 130 140 150
RRAGPRGEIA VDPAAARAAL VTCGGLCPGL NTVLRELVVG LHELYGVRHV
160 170 180 190 200
FGVAAGYRGF YGTDEDHVRL DPAAVDDWHK KGGTVLKTTR GGFDLGKIVD
210 220 230 240 250
GIVARGYTQI YAIGGDGTMR GAVAIFQEFK RRGLNISITG IPKTVDNDIG
260 270 280 290 300
IIDRSFGFQT AVEIAQQAID AAHVEAVSAV NGVGLVKLMG RSTGHIALHA
310 320 330 340 350
TLSSRDVDCC LIPEVDFHLE GKGGLFEFLY ERIKKKGHAV IVVAEGAGQE
360 370 380 390 400
LIPRTDDQKR EQDESGNIVF LDVGPWLKSE LGRWWKREHP DELFTVKYID
410 420 430 440 450
PTYMIRAVPA NATDNLYCTL LAHSAIHGVM AGFTGFVPGP VNGTYSYIPL
460 470 480
EDVAVAKNPV DVNDHKWAWV RSVTNQPDFL KSQA
Length:484
Mass (Da):51,972
Last modified:September 1, 2009 - v1
Checksum:i078F61B4B948AC1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000765 Genomic DNA. Translation: EES12338.1.
RefSeqiXP_002448010.1. XM_002447965.1.

Genome annotation databases

EnsemblPlantsiSb06g019610.1; Sb06g019610.1; Sb06g019610.
GeneIDi8073561.
GrameneiSb06g019610.1; Sb06g019610.1; Sb06g019610.
KEGGisbi:SORBI_06g019610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000765 Genomic DNA. Translation: EES12338.1.
RefSeqiXP_002448010.1. XM_002447965.1.

3D structure databases

ProteinModelPortaliC5YAA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb06g019610.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSb06g019610.1; Sb06g019610.1; Sb06g019610.
GeneIDi8073561.
GrameneiSb06g019610.1; Sb06g019610.1; Sb06g019610.
KEGGisbi:SORBI_06g019610.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
HOGENOMiHOG000016186.
InParanoidiC5YAA9.
KOiK00850.
OrthoDBiEOG093608ZW.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC5YAA9_SORBI
AccessioniPrimary (citable) accession number: C5YAA9
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.