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Protein

ATP-dependent 6-phosphofructokinase

Gene

Sb06g019610

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251ATP; via amide nitrogenUniRule annotation
Metal bindingi216 – 2161Magnesium; catalyticUniRule annotation
Sitei217 – 2171Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei246 – 2461Proton acceptorUniRule annotation
Binding sitei345 – 3451SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi190 – 1912ATPUniRule annotation
Nucleotide bindingi215 – 2184ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: EnsemblPlants/Gramene
  2. ATP binding Source: EnsemblPlants/Gramene

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: EnsemblPlants/Gramene
  2. glycolytic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Sb06g019610Imported
Synonyms:PFKUniRule annotation
ORF Names:SORBIDRAFT_06g019610Imported
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)Imported
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum
ProteomesiUP000000768 Componenti: Chromosome 6

Organism-specific databases

GrameneiC5YAA9.

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi4558.Sb06g019610.1.

Structurei

3D structure databases

ProteinModelPortaliC5YAA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni244 – 2463Substrate bindingUniRule annotation
Regioni289 – 2913Substrate bindingUniRule annotation
Regioni403 – 4064Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiC5YAA9.
KOiK00850.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

C5YAA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPTAARSSG GTPAAAENDA TAPTNTTVTL PPLTLRDVPR LPSALASAAS
60 70 80 90 100
PSPTPAVQNP ISRHPYFHPP ATFYISPGDV TLRHAFFDLA SAAPSPLVAY
110 120 130 140 150
RRAGPRGEIA VDPAAARAAL VTCGGLCPGL NTVLRELVVG LHELYGVRHV
160 170 180 190 200
FGVAAGYRGF YGTDEDHVRL DPAAVDDWHK KGGTVLKTTR GGFDLGKIVD
210 220 230 240 250
GIVARGYTQI YAIGGDGTMR GAVAIFQEFK RRGLNISITG IPKTVDNDIG
260 270 280 290 300
IIDRSFGFQT AVEIAQQAID AAHVEAVSAV NGVGLVKLMG RSTGHIALHA
310 320 330 340 350
TLSSRDVDCC LIPEVDFHLE GKGGLFEFLY ERIKKKGHAV IVVAEGAGQE
360 370 380 390 400
LIPRTDDQKR EQDESGNIVF LDVGPWLKSE LGRWWKREHP DELFTVKYID
410 420 430 440 450
PTYMIRAVPA NATDNLYCTL LAHSAIHGVM AGFTGFVPGP VNGTYSYIPL
460 470 480
EDVAVAKNPV DVNDHKWAWV RSVTNQPDFL KSQA
Length:484
Mass (Da):51,972
Last modified:September 1, 2009 - v1
Checksum:i078F61B4B948AC1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000765 Genomic DNA. Translation: EES12338.1.
RefSeqiXP_002448010.1. XM_002447965.1.

Genome annotation databases

EnsemblPlantsiSb06g019610.1; Sb06g019610.1; Sb06g019610.
GeneIDi8073561.
KEGGisbi:SORBI_06g019610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000765 Genomic DNA. Translation: EES12338.1.
RefSeqiXP_002448010.1. XM_002447965.1.

3D structure databases

ProteinModelPortaliC5YAA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb06g019610.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSb06g019610.1; Sb06g019610.1; Sb06g019610.
GeneIDi8073561.
KEGGisbi:SORBI_06g019610.

Organism-specific databases

GrameneiC5YAA9.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiC5YAA9.
KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. BTx623Imported.

Entry informationi

Entry nameiC5YAA9_SORBI
AccessioniPrimary (citable) accession number: C5YAA9
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: April 29, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.