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C5Y9R0

- LIAS_SORBI

UniProt

C5Y9R0 - LIAS_SORBI

Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi113 – 1131Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi118 – 1181Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi124 – 1241Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi148 – 1481Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi151 – 1511Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIP1UniRule annotation
    Ordered Locus Names:Sb06g018660
    OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
    Taxonomic identifieri4558 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum
    ProteomesiUP000000768: Chromosome 6

    Organism-specific databases

    GrameneiC5Y9R0.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrial matrix Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 386Lipoyl synthase, mitochondrialPRO_0000398856
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi4558.Sb06g018660.1.

    Structurei

    3D structure databases

    ProteinModelPortaliC5Y9R0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OMAiDHRLHSV.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C5Y9R0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHGRRHLAAS LTRALTQAPS RSISSTPSLL QTLDPSVPSP SPPPAAEPGR    50
    LAELRRRLQA DAPSLGDFAY SVEVGTRQRP LPKPKWMKET VPGGAKYAAI 100
    KAKLRELKLH TVCEEARCPN LGECWSGGET GTATATIMIL GDTCTRGCRF 150
    CNVKTSRTPP PPDPDEPSNV AQAIASWGLE YIVITSVDRD DLPDQGSGHF 200
    AETVQKLKAL KPEMLIEALV PDFRGDPSCV EKVATSGLHV FAHNIETVEE 250
    LQRNVRDYRA NFKQSIDVLK MAKEYAPPGT LTKTSIMLGC GETPDQVIST 300
    MEKVRAAGVD VITFGQYMRP SKRHMPVSEY VTPEAFEKYR ALGVEMGFRY 350
    VASGPMVRSS YKAGEFYIKA MIEADRSKAT TADSSA 386
    Length:386
    Mass (Da):42,141
    Last modified:September 1, 2009 - v1
    Checksum:iA19F3845489C859C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000765 Genomic DNA. Translation: EES12278.1.
    RefSeqiXP_002447950.1. XM_002447905.1.
    UniGeneiSbi.15755.

    Genome annotation databases

    EnsemblPlantsiSb06g018660.1; Sb06g018660.1; Sb06g018660.
    GeneIDi8057459.
    KEGGisbi:SORBI_06g018660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000765 Genomic DNA. Translation: EES12278.1 .
    RefSeqi XP_002447950.1. XM_002447905.1.
    UniGenei Sbi.15755.

    3D structure databases

    ProteinModelPortali C5Y9R0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4558.Sb06g018660.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Sb06g018660.1 ; Sb06g018660.1 ; Sb06g018660 .
    GeneIDi 8057459.
    KEGGi sbi:SORBI_06g018660.

    Organism-specific databases

    Gramenei C5Y9R0.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OMAi DHRLHSV.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03128. Lipoyl_synth_plantM.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027527. Lipoyl_synth_mt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. BTx623.

    Entry informationi

    Entry nameiLIAS_SORBI
    AccessioniPrimary (citable) accession number: C5Y9R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3