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Protein

Lipoyl synthase, chloroplastic

Gene

LIP1P

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi131 – 1311Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi135 – 1351Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, chloroplasticUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoate synthase, plastidialUniRule annotation
Short name:
LIP1pUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LIP1PUniRule annotation
Ordered Locus Names:Sb03g035760
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum
ProteomesiUP000000768: Chromosome 3

Organism-specific databases

GrameneiC5XKZ1.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 368Lipoyl synthase, chloroplasticPRO_0000398870
Transit peptidei1 – ?ChloroplastUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi4558.Sb03g035760.1.

Structurei

3D structure databases

ProteinModelPortaliC5XKZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiC5XKZ1.
KOiK03644.
OMAiCREGMIT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5XKZ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSSLARPLR PPVLAGCGGR RGHGAPRGSV SVARCRAEAA PPTVGTASRA
60 70 80 90 100
PAGPYTGRDP EVKKPAWLRQ RAAQGDKYAR LRESIGELKL NTVCVEAQCP
110 120 130 140 150
NIGECWNGGG GAGGEGDGIA TATIMVLGDT CTRGCRFCAV KTSNKPPPPD
160 170 180 190 200
PLEPLNTALA VASWGVDYVV LTSVDRDDLP DGGSSHFAQT VRALKELKPG
210 220 230 240 250
ILVECLTSDF RGDLEAVSSL ANSGLDVYAH NIETVRSLQR IVRDPRAGYD
260 270 280 290 300
QSLAVLKHAK DCREGMITKS SIMLGLGETD EEVKQAMIDL RAIGVDILTL
310 320 330 340 350
GQYLQPTERH LTVREYVTPE KFQFWKEYGE SVGFRYVASG PLVRSSYRAG
360
ELFVQNLVRN NKTGSSSS
Length:368
Mass (Da):39,528
Last modified:September 1, 2009 - v1
Checksum:iB63753E5085C935C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA. Translation: EES01528.1.
RefSeqiXP_002456408.1. XM_002456363.1.

Genome annotation databases

EnsemblPlantsiSb03g035760.1; Sb03g035760.1; Sb03g035760.
GeneIDi8072124.
KEGGisbi:SORBI_03g035760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA. Translation: EES01528.1.
RefSeqiXP_002456408.1. XM_002456363.1.

3D structure databases

ProteinModelPortaliC5XKZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb03g035760.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSb03g035760.1; Sb03g035760.1; Sb03g035760.
GeneIDi8072124.
KEGGisbi:SORBI_03g035760.

Organism-specific databases

GrameneiC5XKZ1.

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiC5XKZ1.
KOiK03644.
OMAiCREGMIT.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. BTx623.

Entry informationi

Entry nameiLISC_SORBI
AccessioniPrimary (citable) accession number: C5XKZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 1, 2009
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.