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Protein

ATP-dependent 6-phosphofructokinase

Gene

Sb03g034060

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathway:iglycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Sb02g027000)
  3. ATP-dependent 6-phosphofructokinase (Sb01g022370), ATP-dependent 6-phosphofructokinase (Sb09g006030), ATP-dependent 6-phosphofructokinase (Sb07g021500), ATP-dependent 6-phosphofructokinase (Sb03g003140), ATP-dependent 6-phosphofructokinase (Sb02g024680), ATP-dependent 6-phosphofructokinase (Sb10g003650), ATP-dependent 6-phosphofructokinase (Sb03g034060), ATP-dependent 6-phosphofructokinase (Sb06g019610), ATP-dependent 6-phosphofructokinase (Sb01g004810), ATP-dependent 6-phosphofructokinase (Sb09g026150)
  4. Fructose-bisphosphate aldolase (Sb10g023850), Fructose-bisphosphate aldolase (Sb03g043140), Fructose-bisphosphate aldolase (Sb03g008050), Fructose-bisphosphate aldolase (Sb08g004500), Fructose-bisphosphate aldolase (Sb04g019020), Fructose-bisphosphate aldolase (Sb05g004590)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811ATP; via amide nitrogenUniRule annotation
Metal bindingi270 – 2701Magnesium; catalyticUniRule annotation
Sitei271 – 2711Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei300 – 3001Proton acceptorUniRule annotation
Binding sitei399 – 3991SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi244 – 2452ATPUniRule annotation
Nucleotide bindingi269 – 2724ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Sb03g034060Imported
Synonyms:PFKUniRule annotation
ORF Names:SORBIDRAFT_03g034060Imported
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)Imported
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum
ProteomesiUP000000768 Componenti: Chromosome 3

Organism-specific databases

GrameneiC5XJ72.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi4558.Sb03g034060.1.

Structurei

3D structure databases

ProteinModelPortaliC5XJ72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3003Substrate bindingUniRule annotation
Regioni343 – 3453Substrate bindingUniRule annotation
Regioni455 – 4584Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiC5XJ72.
KOiK00850.
OMAiVEEDHDS.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

C5XJ72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSHIILPK EEEEEEKEAA AVAEAGLGVG VEADHDSPAQ RPYQEQAPGK
60 70 80 90 100
AALPFSATCV RISRDSYPNL RALRNASTMA LHDDDAAFVK IEEGDFGYVL
110 120 130 140 150
DDVPHLTDYL PDLPTFPNPL QDHPAYSTVK QYFVNADDTV PEKVVVQKNS
160 170 180 190 200
PRGVHFRRAG PRQRVYFESE EVKACIVTCG GLCPGLNTVI RELVCGLSHM
210 220 230 240 250
YNVSNVFGIQ NGYKGFYSSN YLPMTPKSVN DIHKRGGTVL GTSRGGHDTK
260 270 280 290 300
KIVDNIQDRG INQVYIIGGD GTQKGAYEIY KEIRRRGLHV AVAGIPKTID
310 320 330 340 350
NDIAVIDKSF GFDTAVEEAQ RAINAAHVEA SSAENGIGLV KLMGRYSGFI
360 370 380 390 400
AMYATLASRD VDCCLIPESP FYLEGEGGLF EYIDRRLKEN NHMVIVVAEG
410 420 430 440 450
AGQDLIAQSI PAADQQDASG NKLLLDVGLW LTHKIKDYCK SKKMEMTIKY
460 470 480 490 500
IDPTYMIRAI PSNASDNVYC TLLAHSAIHG AMAGYSFTVG MVNGRHAYIP
510 520 530 540 550
FHRVTSTRNK VRITDRMWAR LLSSTNQPSF LSQKDIDAAR EADKAANAKN
560
QSAPALANGE K
Length:561
Mass (Da):61,494
Last modified:September 1, 2009 - v1
Checksum:i67CCBEA64492641A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA. Translation: EES03580.1.
RefSeqiXP_002458460.1. XM_002458415.1.
UniGeneiSbi.15083.

Genome annotation databases

EnsemblPlantsiSb03g034060.1; Sb03g034060.1; Sb03g034060.
GeneIDi8062161.
KEGGisbi:SORBI_03g034060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000762 Genomic DNA. Translation: EES03580.1.
RefSeqiXP_002458460.1. XM_002458415.1.
UniGeneiSbi.15083.

3D structure databases

ProteinModelPortaliC5XJ72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb03g034060.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSb03g034060.1; Sb03g034060.1; Sb03g034060.
GeneIDi8062161.
KEGGisbi:SORBI_03g034060.

Organism-specific databases

GrameneiC5XJ72.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000016186.
InParanoidiC5XJ72.
KOiK00850.
OMAiVEEDHDS.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. BTx623Imported.

Entry informationi

Entry nameiC5XJ72_SORBI
AccessioniPrimary (citable) accession number: C5XJ72
Entry historyi
Integrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: May 27, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.