ID   C5XEX3_SORBI            Unreviewed;      1038 AA.
AC   C5XEX3;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   27-MAR-2024, entry version 76.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   ORFNames=SORBI_3003G100600 {ECO:0000313|EMBL:EES00444.1};
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558 {ECO:0000313|EMBL:EES00444.1, ECO:0000313|Proteomes:UP000000768};
RN   [1] {ECO:0000313|EMBL:EES00444.1, ECO:0000313|Proteomes:UP000000768}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. BTx623 {ECO:0000313|Proteomes:UP000000768};
RX   PubMed=19189423; DOI=10.1038/nature07723;
RA   Paterson A.H., Bowers J.E., Bruggmann R., Dubchak I., Grimwood J.,
RA   Gundlach H., Haberer G., Hellsten U., Mitros T., Poliakov A., Schmutz J.,
RA   Spannagl M., Tang H., Wang X., Wicker T., Bharti A.K., Chapman J.,
RA   Feltus F.A., Gowik U., Grigoriev I.V., Lyons E., Maher C.A., Martis M.,
RA   Narechania A., Otillar R.P., Penning B.W., Salamov A.A., Wang Y., Zhang L.,
RA   Carpita N.C., Freeling M., Gingle A.R., Hash C.T., Keller B., Klein P.,
RA   Kresovich S., McCann M.C., Ming R., Peterson D.G., Mehboob-ur-Rahman,
RA   Ware D., Westhoff P., Mayer K.F., Messing J., Rokhsar D.S.;
RT   "The Sorghum bicolor genome and the diversification of grasses.";
RL   Nature 457:551-556(2009).
RN   [2] {ECO:0000313|Proteomes:UP000000768}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. BTx623 {ECO:0000313|Proteomes:UP000000768};
RX   PubMed=29161754; DOI=10.1111/tpj.13781;
RA   McCormick R.F., Truong S.K., Sreedasyam A., Jenkins J., Shu S., Sims D.,
RA   Kennedy M., Amirebrahimi M., Weers B.D., McKinley B., Mattison A.,
RA   Morishige D.T., Grimwood J., Schmutz J., Mullet J.E.;
RT   "The Sorghum bicolor reference genome: improved assembly, gene annotations,
RT   a transcriptome atlas, and signatures of genome organization.";
RL   Plant J. 93:338-354(2018).
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003774}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; CM000762; EES00444.1; -; Genomic_DNA.
DR   RefSeq; XP_002455324.1; XM_002455279.1.
DR   AlphaFoldDB; C5XEX3; -.
DR   STRING; 4558.C5XEX3; -.
DR   EnsemblPlants; EES00444; EES00444; SORBI_3003G100600.
DR   GeneID; 8079083; -.
DR   Gramene; EES00444; EES00444; SORBI_3003G100600.
DR   KEGG; sbi:8079083; -.
DR   eggNOG; ENOG502QPVS; Eukaryota.
DR   HOGENOM; CLU_006557_2_0_1; -.
DR   InParanoid; C5XEX3; -.
DR   OMA; FGWTQSR; -.
DR   OrthoDB; 355614at2759; -.
DR   Proteomes; UP000000768; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 2.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 2.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Photosynthesis {ECO:0000256|ARBA:ARBA00022531};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000768}.
FT   REGION          396..420
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        154
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        704
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   1038 AA;  115970 MW;  08E1BB43B6494941 CRC64;
     MLDTTDDIAE GISFQAFEDD CRLLASLLHD VLLRELGPRF IQILERNRIL AQSAVSMRAA
     GMEDTAAVVE RQLEADLAAM SLDDALCVAR AFSHYLNLMG IAETHHRVRK ARNIEQLSKS
     CDDIFNKLIQ SGVPPEQLYD TVCKQEVEIV LTAHPTQINR RTLQYKHLRV AHLLEFNGRP
     DLSHEDKEML IEDLVREITA IWQTDELRRH KPTPVDEARA GLHIVEQSLW KAIPHYLRRV
     SSALKKHTGR PLPLTCTPIK FGSWMGGDRD GNPNVTAKVT RDVSLLSRWM AIDFYIRELD
     NLSFELSIKR CSDKVASLAN DIILKESASE DLKANTWNKT VPQNNAKLHH NLALPAQLPS
     GADLPSCTEC SDGESQFRMI NLPRNSSRPG ALNLTEKIED SPLSSPTSRQ PQLSRTSSGH
     QLRKLFKESH IGRSSSFQKL LEPSLSDRPG ITPYRVVLGN VKEKLVKTRR RLELLLEDLP
     CDYDTEEYCE TSDQLLEPLL LCHQSLQSCG ASVLADGRLA DLIRRVATFG MVLMKLDVRQ
     ESGRHTEALD SVTSYLDLGV YSEWDEEKKL DFLTRELKGK RPLVPQNIEV SADVKEVLDT
     FKVAAELGSD SLGAYVISMA SNASDVLAVE LLQKDARLTV SGDLGRPCPG GTLRVVPLFE
     TVKDLQAAGS AIRKLLSIDW YREHIIKNHN GHQEVMVGYS DSGKDAGRFT AAWELYKAQE
     DVVAACNEFG IKVTLFHGRG GSIGRGGGPT YLAIQSQPPG SVMGTLRSTE QGEMVQAKFG
     LPQTAVRQLE IYTTAVLLAT LRPPHPPRDP NWRTVMEEIS RASCAHYRAT VYEDPEFITY
     FQEATPQAEL GHLNIGSRPA KRKQALGGIS SLRAIPWVFA WTQTRLVLPA WLGVGTGLQD
     ALDRGHGAEL RAMYGEWPFF QSTVDLIEMV TAKADAPVAA HYEEMLVAPE RRAVGERLRK
     ELARTERCVL AVSGHSKLTA HNRSLRRLIE SRLAYLNPIN MLQVEVLRRL RRDDDNRRLR
     DALLITINGI AAGMRNTG
//