ID   C5X6W5_SORBI            Unreviewed;       402 AA.
AC   C5X6W5;
DT   01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   01-SEP-2009, sequence version 1.
DT   24-JAN-2024, entry version 73.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4 {ECO:0000256|RuleBase:RU364141};
DE   AltName: Full=Mediator complex subunit 4 {ECO:0000256|RuleBase:RU364141};
GN   Name=MED4 {ECO:0000256|RuleBase:RU364141};
GN   ORFNames=SORBI_3002G280600 {ECO:0000313|EMBL:EER99271.1};
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558 {ECO:0000313|EMBL:EER99271.1, ECO:0000313|Proteomes:UP000000768};
RN   [1] {ECO:0000313|EMBL:EER99271.1, ECO:0000313|Proteomes:UP000000768}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. BTx623 {ECO:0000313|Proteomes:UP000000768};
RX   PubMed=19189423; DOI=10.1038/nature07723;
RA   Paterson A.H., Bowers J.E., Bruggmann R., Dubchak I., Grimwood J.,
RA   Gundlach H., Haberer G., Hellsten U., Mitros T., Poliakov A., Schmutz J.,
RA   Spannagl M., Tang H., Wang X., Wicker T., Bharti A.K., Chapman J.,
RA   Feltus F.A., Gowik U., Grigoriev I.V., Lyons E., Maher C.A., Martis M.,
RA   Narechania A., Otillar R.P., Penning B.W., Salamov A.A., Wang Y., Zhang L.,
RA   Carpita N.C., Freeling M., Gingle A.R., Hash C.T., Keller B., Klein P.,
RA   Kresovich S., McCann M.C., Ming R., Peterson D.G., Mehboob-ur-Rahman,
RA   Ware D., Westhoff P., Mayer K.F., Messing J., Rokhsar D.S.;
RT   "The Sorghum bicolor genome and the diversification of grasses.";
RL   Nature 457:551-556(2009).
RN   [2] {ECO:0000313|EMBL:EER99271.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Paterson A., Mullet J., Bowers J., Bruggmann R., Dubchak I., Grimwood J.,
RA   Gundlach H., Haberer G., Hellsten U., Mitros T., Poliakov A., Schmutz J.,
RA   Spannagl M., Tang H., Wang X., Wicker T., Bharti A., Chapman J., Feltus F.,
RA   Gowik U., Grigoriev I., Lyons E., Maher C., Martis M., Narechania A.,
RA   Otillar R., Penning B., Salamov A., Wang Y., Zhang L., Carpita N.,
RA   Freeling M., Gingle A., Hash C., Keller B., Klein P., Kresovich S.,
RA   Mccann M., Ming R., Peterson D., Rahman M., Ware D., Westhoff P., Mayer K.,
RA   Messing J., Sims D., Jenkins J., Shu S., Rokhsar D.;
RT   "WGS assembly of Sorghum bicolor.";
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|Proteomes:UP000000768}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. BTx623 {ECO:0000313|Proteomes:UP000000768};
RX   PubMed=29161754; DOI=10.1111/tpj.13781;
RA   McCormick R.F., Truong S.K., Sreedasyam A., Jenkins J., Shu S., Sims D.,
RA   Kennedy M., Amirebrahimi M., Weers B.D., McKinley B., Mattison A.,
RA   Morishige D.T., Grimwood J., Schmutz J., Mullet J.E.;
RT   "The Sorghum bicolor reference genome: improved assembly, gene annotations,
RT   a transcriptome atlas, and signatures of genome organization.";
RL   Plant J. 93:338-354(2018).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. {ECO:0000256|RuleBase:RU364141}.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000256|RuleBase:RU364141}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC       ECO:0000256|RuleBase:RU364141}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000256|ARBA:ARBA00009626, ECO:0000256|RuleBase:RU364141}.
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DR   EMBL; CM000761; EER99271.1; -; Genomic_DNA.
DR   EMBL; CM000761; KXG36089.1; -; Genomic_DNA.
DR   EMBL; CM000761; OQU89839.1; -; Genomic_DNA.
DR   RefSeq; XP_002462750.1; XM_002462705.1.
DR   AlphaFoldDB; C5X6W5; -.
DR   STRING; 4558.C5X6W5; -.
DR   EnsemblPlants; EER99271; EER99271; SORBI_3002G280600.
DR   EnsemblPlants; KXG36089; KXG36089; SORBI_3002G280600.
DR   EnsemblPlants; OQU89839; OQU89839; SORBI_3002G280600.
DR   Gramene; EER99271; EER99271; SORBI_3002G280600.
DR   Gramene; KXG36089; KXG36089; SORBI_3002G280600.
DR   Gramene; OQU89839; OQU89839; SORBI_3002G280600.
DR   eggNOG; ENOG502QQ6I; Eukaryota.
DR   HOGENOM; CLU_057409_1_0_1; -.
DR   InParanoid; C5X6W5; -.
DR   OMA; NPDMQDE; -.
DR   OrthoDB; 473091at2759; -.
DR   Proteomes; UP000000768; Chromosome 2.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblPlants.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4; 1.
DR   PANTHER; PTHR13208:SF2; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator {ECO:0000256|RuleBase:RU364141};
KW   Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364141};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000768};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163,
KW   ECO:0000256|RuleBase:RU364141};
KW   Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW   ECO:0000256|RuleBase:RU364141}.
FT   REGION          1..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          192..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          323..402
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        20..37
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        323..337
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        377..391
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   402 AA;  42767 MW;  3D4F58BDC17C0D6A CRC64;
     MMQSHLPSPA RLGLTASSPS LPPNPVPLNP TSSPPPGSLP SSAAAGASAA PTLTTSPSLL
     PLLPPLPRAQ SLLQLISSLA SNLFELSPNR AAWIQAYRGS FPTFLPSASS SAPPLPTPIS
     STKDALSLLT TLQTQLFEAV AELQETLDLQ DARARLAREA RAKDAALVAF AKKLHEAHQV
     LDRLVDDYAD YRRDPKRPRG AAAADDPEPA SEGDFGTSLH SSLKLDDILA YAHRISYTTF
     APPEHGAGLP LRVSLPPVPQ ENQMRASQLY LFADLDVGVP KKPLDAKEGI AAEVEAAPLY
     EPPQEGPSRL PNTLPPMFPK ELKPPPGWKP GDPITLPPDE ILPGIKGEEP QAPVPQPHVP
     VRPVAPMGAE PIQVQPVQLD FDSSSSDEYS SDVGSSEEDD ED
//