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C5W716

- NUOK_ECOBD

UniProt

C5W716 - NUOK_ECOBD

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Protein

NADH-quinone oxidoreductase subunit K

Gene

nuoK

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

Catalytic activityi

NADH + quinone = NAD+ + quinol.UniRule annotation

GO - Molecular functioni

  1. NADH dehydrogenase (quinone) activity Source: UniProtKB-HAMAP
  2. quinone binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
  2. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Transport

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit KUniRule annotation (EC:1.6.99.5UniRule annotation)
Alternative name(s):
NADH dehydrogenase I subunit KUniRule annotation
NDH-1 subunit KUniRule annotation
Gene namesi
Name:nuoKUniRule annotation
Ordered Locus Names:B21_02164, ECBD_1382, ECD_02204
OrganismiEscherichia coli (strain B / BL21-DE3)
Taxonomic identifieri469008 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001509: Chromosome, UP000002032: Chromosome, UP000009074: Chromosome

Subcellular locationi

Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalUniRule annotationAdd
BLAST
Transmembranei28 – 4821HelicalUniRule annotationAdd
BLAST
Transmembranei60 – 8021HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100NADH-quinone oxidoreductase subunit KPRO_0000390042Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59176N.
STRINGi469008.ECBD_1382.

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 2421Combined sources
Helixi28 – 5225Combined sources
Helixi57 – 8226Combined sources
Turni83 – 864Combined sources
Helixi92 – 943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RKOX-ray3.00G/K1-100[»]
ProteinModelPortaliC5W716.
SMRiC5W716. Positions 1-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4L family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0713.
HOGENOMiHOG000066429.
KOiK00340.
OMAiADGQIMF.

Family and domain databases

HAMAPiMF_01456. NDH1_NuoK.
InterProiIPR001133. NADH_UbQ_OxRdtase_chain4L/K.
[Graphical view]
PANTHERiPTHR11434. PTHR11434. 1 hit.
PfamiPF00420. Oxidored_q2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5W716-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIPLQHGLIL AAILFVLGLT GLVIRRNLLF MLIGLEIMIN ASALAFVVAG
60 70 80 90 100
SYWGQTDGQV MYILAISLAA AEASIGLALL LQLHRRRQNL NIDSVSEMRG
Length:100
Mass (Da):10,845
Last modified:September 1, 2009 - v1
Checksum:iCC5AF3946A879383
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946981 Genomic DNA. Translation: CAQ32681.1.
CP001665 Genomic DNA. Translation: ACT28445.1.
CP001509 Genomic DNA. Translation: ACT44026.1.
RefSeqiYP_002999943.1. NC_012892.2.
YP_003035630.1. NC_012947.1.
YP_003054797.1. NC_012971.2.

Genome annotation databases

EnsemblBacteriaiACT28445; ACT28445; ECBD_1382.
ACT44026; ACT44026; ECD_02204.
CAQ32681; CAQ32681; B21_02164.
GeneIDi8114248.
8156286.
8181254.
KEGGiebd:ECBD_1382.
ebe:B21_02164.
ebl:ECD_02204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946981 Genomic DNA. Translation: CAQ32681.1 .
CP001665 Genomic DNA. Translation: ACT28445.1 .
CP001509 Genomic DNA. Translation: ACT44026.1 .
RefSeqi YP_002999943.1. NC_012892.2.
YP_003035630.1. NC_012947.1.
YP_003054797.1. NC_012971.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RKO X-ray 3.00 G/K 1-100 [» ]
ProteinModelPortali C5W716.
SMRi C5W716. Positions 1-100.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59176N.
STRINGi 469008.ECBD_1382.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACT28445 ; ACT28445 ; ECBD_1382 .
ACT44026 ; ACT44026 ; ECD_02204 .
CAQ32681 ; CAQ32681 ; B21_02164 .
GeneIDi 8114248.
8156286.
8181254.
KEGGi ebd:ECBD_1382.
ebe:B21_02164.
ebl:ECD_02204.

Phylogenomic databases

eggNOGi COG0713.
HOGENOMi HOG000066429.
KOi K00340.
OMAi ADGQIMF.

Family and domain databases

HAMAPi MF_01456. NDH1_NuoK.
InterProi IPR001133. NADH_UbQ_OxRdtase_chain4L/K.
[Graphical view ]
PANTHERi PTHR11434. PTHR11434. 1 hit.
Pfami PF00420. Oxidored_q2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequencing and gene expression analysis of Escherichia coli BL21."
    Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.
    Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B / BL21-DE3.
  2. "Complete sequence of Escherichia coli BL21(DE3)."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
    Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B / BL21-DE3.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: B / BL21-DE3.

Entry informationi

Entry nameiNUOK_ECOBD
AccessioniPrimary (citable) accession number: C5W716
Secondary accession number(s): C6E9S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: September 1, 2009
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3