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C5W716

- NUOK_ECOBD

UniProt

C5W716 - NUOK_ECOBD

Protein

NADH-quinone oxidoreductase subunit K

Gene

nuoK

Organism
Escherichia coli (strain B / BL21-DE3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.UniRule annotation

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    GO - Molecular functioni

    1. NADH dehydrogenase (quinone) activity Source: UniProtKB-HAMAP
    2. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro
    2. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit KUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit KUniRule annotation
    NDH-1 subunit KUniRule annotation
    Gene namesi
    Name:nuoKUniRule annotation
    Ordered Locus Names:B21_02164, ECBD_1382, ECD_02204
    OrganismiEscherichia coli (strain B / BL21-DE3)
    Taxonomic identifieri469008 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001509: Chromosome, UP000002032: Chromosome, UP000009074: Chromosome

    Subcellular locationi

    Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 100100NADH-quinone oxidoreductase subunit KPRO_0000390042Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-59176N.
    STRINGi469008.ECBD_1382.

    Structurei

    Secondary structure

    1
    100
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2421
    Helixi28 – 5225
    Helixi57 – 8226
    Turni83 – 864
    Helixi92 – 943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RKOX-ray3.00G/K1-100[»]
    ProteinModelPortaliC5W716.
    SMRiC5W716. Positions 1-100.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421HelicalUniRule annotationAdd
    BLAST
    Transmembranei28 – 4821HelicalUniRule annotationAdd
    BLAST
    Transmembranei60 – 8021HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 4L family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0713.
    HOGENOMiHOG000066429.
    KOiK00340.
    OMAiADGQIMF.

    Family and domain databases

    HAMAPiMF_01456. NDH1_NuoK.
    InterProiIPR001133. NADH_UbQ_OxRdtase_chain4L/K.
    [Graphical view]
    PANTHERiPTHR11434. PTHR11434. 1 hit.
    PfamiPF00420. Oxidored_q2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5W716-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPLQHGLIL AAILFVLGLT GLVIRRNLLF MLIGLEIMIN ASALAFVVAG    50
    SYWGQTDGQV MYILAISLAA AEASIGLALL LQLHRRRQNL NIDSVSEMRG 100
    Length:100
    Mass (Da):10,845
    Last modified:September 1, 2009 - v1
    Checksum:iCC5AF3946A879383
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM946981 Genomic DNA. Translation: CAQ32681.1.
    CP001665 Genomic DNA. Translation: ACT28445.1.
    CP001509 Genomic DNA. Translation: ACT44026.1.
    RefSeqiYP_002999943.1. NC_012892.2.
    YP_003035630.1. NC_012947.1.
    YP_003054797.1. NC_012971.2.

    Genome annotation databases

    EnsemblBacteriaiACT28445; ACT28445; ECBD_1382.
    ACT44026; ACT44026; ECD_02204.
    CAQ32681; CAQ32681; B21_02164.
    GeneIDi8114248.
    8156286.
    8181254.
    KEGGiebd:ECBD_1382.
    ebe:B21_02164.
    ebl:ECD_02204.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM946981 Genomic DNA. Translation: CAQ32681.1 .
    CP001665 Genomic DNA. Translation: ACT28445.1 .
    CP001509 Genomic DNA. Translation: ACT44026.1 .
    RefSeqi YP_002999943.1. NC_012892.2.
    YP_003035630.1. NC_012947.1.
    YP_003054797.1. NC_012971.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RKO X-ray 3.00 G/K 1-100 [» ]
    ProteinModelPortali C5W716.
    SMRi C5W716. Positions 1-100.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59176N.
    STRINGi 469008.ECBD_1382.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACT28445 ; ACT28445 ; ECBD_1382 .
    ACT44026 ; ACT44026 ; ECD_02204 .
    CAQ32681 ; CAQ32681 ; B21_02164 .
    GeneIDi 8114248.
    8156286.
    8181254.
    KEGGi ebd:ECBD_1382.
    ebe:B21_02164.
    ebl:ECD_02204.

    Phylogenomic databases

    eggNOGi COG0713.
    HOGENOMi HOG000066429.
    KOi K00340.
    OMAi ADGQIMF.

    Family and domain databases

    HAMAPi MF_01456. NDH1_NuoK.
    InterProi IPR001133. NADH_UbQ_OxRdtase_chain4L/K.
    [Graphical view ]
    PANTHERi PTHR11434. PTHR11434. 1 hit.
    Pfami PF00420. Oxidored_q2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and gene expression analysis of Escherichia coli BL21."
      Leparc G., Striedner G., Bayer K., Kreil D., Krempl P.M.
      Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B / BL21-DE3.
    2. "Complete sequence of Escherichia coli BL21(DE3)."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.
      Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B / BL21-DE3.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: B / BL21-DE3.

    Entry informationi

    Entry nameiNUOK_ECOBD
    AccessioniPrimary (citable) accession number: C5W716
    Secondary accession number(s): C6E9S3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3