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Protein

Biotin synthase

Gene

bioB

Organism
Neisseria flavescens SK114
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi112Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi115Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi152Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi183Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi243Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi315Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:NEIFL0001_1190Imported
OrganismiNeisseria flavescens SK114Imported
Taxonomic identifieri596320 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000005533 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi596320.NEIFL0001_1190.

Structurei

3D structure databases

ProteinModelPortaliC5TMU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 304Elp3InterPro annotationAdd BLAST207
Domaini275 – 367BATSInterPro annotationAdd BLAST93

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

C5TMU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRRFRRPFI LISNLLLPSL RRQSSRQRHC VAVNTKEHTM TVSPVALRRQ
60 70 80 90 100
TDRKPHPTAR YWKKCDVEAL FGLPFLDLVF QAAEIHRQNF NPREIQLSTL
110 120 130 140 150
LSIKTGGCPE DCAYCPQSAH HNTNLGKEQM MDVDEIVEKA KIAQSRGASR
160 170 180 190 200
FCMGAAWRGP KPKDVAVVSE IISAVKGLGM EVCGTFGMLE DGMAEDFKKA
210 220 230 240 250
GLDYYNHNLD TDPERYNDII HTRKHEDRMD TLGKVRNAGL KVCCGGIVGM
260 270 280 290 300
NETRAERAGL IASLANLDPQ PESVPINQLV KVEGTPLADA EDLDWTEFVR
310 320 330 340 350
TIAVARITMP HSYVRLSAGR SNMPESMQAM CFMAGANSIF YGDKLLTTGN
360 370 380
PDEDGDRLLM EKLNLYPLRF ELEEEYKAAQ ETPKIKVDY
Length:389
Mass (Da):43,763
Last modified:September 1, 2009 - v1
Checksum:i3D928A5D46052B61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACQV01000024 Genomic DNA. Translation: EER55871.1.

Genome annotation databases

EnsemblBacteriaiEER55871; EER55871; NEIFL0001_1190.

Similar proteinsi

Entry informationi

Entry nameiC5TMU2_NEIFL
AccessioniPrimary (citable) accession number: C5TMU2
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 22, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported