Reviewed, UniProtKB/Swiss-Prot C5P230 (CHI1_COCP7)

Names and origin

Protein names

Recommended name:
    Endochitinase 1
    EC=3.2.1.14
Alternative name(s):
    Complement-fixation antigen
      Short name=CF-antigen
      Short name=CF-AG
    CiX1

Gene names

Name:	CTS1
ORF Names:	CPC735_036390

Organism

Coccidioides posadasii (strain C735) (Valley fever fungus)

Taxonomic identifier

222929 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Sequence caution	The sequence AAA92643.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 427	410	Endochitinase 1		PRO_0000387962
Sites
	Active site	171	1	Proton donor By similarity
	Site	169	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	103	1	K → N in AAA92643. Ref.1
	Sequence conflict	109	1	T → I in AAA92643. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	C5P230-1 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 7DDA4A82DD67A21B	FASTA	427	47,400
	10 20 30 40 50 60 MRFLIGALLT LQTLVQASSM SSMPNSYPVP EAPAEGGFRS VVYFVNWAIY GRGHNPQDLK 70 80 90 100 110 120 ADQFTHILYA FANIRPSGEV YLSDTWADTD KHYPGDKWDE PGKNVYGCTK QMYLLKKNNR 130 140 150 160 170 180 NLKTLLSIGG WTYSPNFKTP ASTEEGRKKF ADTSLKLMKD LGFDGIDIDW EYPEDEKQAN 190 200 210 220 230 240 DFVLLLKACR EALDAYSAKH PNGKKFLLTI ASPAGPQNYN KLKLAEMDKY LDFWNLMAYD 250 260 270 280 290 300 FSGSWDKVSG HMSNVFPSTT KPESTPFSSD KAVKDYIKAG VPANKIVLGM PLYGRAFAST 310 320 330 340 350 360 DGIGTSFNGV GGGSWENGVW DYKDMPQQGA QVTELEDIAA SYSYDKNKRY LISYDTVKIA 370 380 390 400 410 420 GKKAEYITKN GMGGGMWWES SSDKTGNESL VGTVVNGLGG TGKLEQRENE LSYPESVYDN LKNGMPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two chitinase-encoding genes (cts1, cts2) from the fungus Coccidioides immitis." Pishko E.J., Kirkland T.N., Cole G.T. Gene 167:173-177(1995) [PubMed: 8566773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed: 19717792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L41663 Genomic DNA. Translation: AAA92643.1. Sequence problems. ACFW01000012 Genomic DNA. Translation: EER28933.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR011583. Chitinase_II. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SMART	SM00636. Glyco_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_COCP7

Accession

Primary (citable) accession number: C5P230
Secondary accession number(s): P54196 , Q00432, Q00435, Q400W4, Q400W5, Q400W6, Q9C0M7, Q9C2W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	September 1, 2009
Last modified:	November 24, 2009
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)