Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent 6-phosphofructokinase

Gene

CTRG_05115

Organism
Candida tropicalis (strain ATCC MYA-3404 / T1) (Yeast)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (CTRG_00601)
  3. ATP-dependent 6-phosphofructokinase (CTRG_06094), ATP-dependent 6-phosphofructokinase (CTRG_05115)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192ATP; via amide nitrogenUniRule annotation1
Metal bindingi287Magnesium; catalyticUniRule annotation1
Active sitei334Proton acceptorUniRule annotation1
Binding sitei369Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei433SubstrateUniRule annotation1
Binding sitei461Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei644Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei833Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei920Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 257ATPUniRule annotation2
Nucleotide bindingi286 – 289ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
ORF Names:CTRG_05115Imported
OrganismiCandida tropicalis (strain ATCC MYA-3404 / T1) (Yeast)Imported
Taxonomic identifieri294747 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000002037 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.UniRule annotation

Protein-protein interaction databases

STRINGi294747.XP_002550817.1.

Structurei

3D structure databases

ProteinModelPortaliC5MGC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 493PFKInterPro annotationAdd BLAST310
Domaini574 – 865PFKInterPro annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 559N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST559
Regioni332 – 334Substrate bindingUniRule annotation3
Regioni376 – 378Substrate bindingUniRule annotation3
Regioni467 – 470Substrate bindingUniRule annotation4
Regioni560 – 573Interdomain linkerUniRule annotationAdd BLAST14
Regioni574 – 943C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST370
Regioni702 – 706Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni747 – 749Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni839 – 842Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.
OrthoDBiEOG092C0LOE.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5MGC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISIVNGTST HSLVAGTKET LSQAINFYTN ILGLSVHSEN DDLTYLSNED
60 70 80 90 100
NKMIVKIKLD AKTGLSPSQI HDRREDIISK LNVIDWRSLD TTNVFKVENL
110 120 130 140 150
VALIETLSTY RYTLQITPNE LYPNEVYCVD PLGYIVGFTA CAEPLTLVPP
160 170 180 190 200
LQKANPKFDG ASNLMSKSGS QSRSVEEIKQ VRRNIGVMTS GGDSQGMNAA
210 220 230 240 250
VRAVVRATIF RGCKAFVIKE GYEGLVKGGP EYIKETNWQD VRGYLSEGGT
260 270 280 290 300
NIGTARCMEF KERWGRLLGC RHLIEAGIDG LIVCGGDGSL TGADLFRHEW
310 320 330 340 350
PSLIAELKEK GEITAEQYER HKHLYICGMV GSIDNDMAMT DSTIGGYSAL
360 370 380 390 400
ERICRAVDYI DATASSHSRA FVVEVMGRHC GWLALMAGIA TSADYILIPE
410 420 430 440 450
KPASSKDWKD QMCDIVGKHR AKGKRKTIVI VAEGAITSEL KPITSEEVKD
460 470 480 490 500
VLVDRLGLDT RITVLGHVQR GGTAVAYDRI LATLQGVEAV KAILELTPDV
510 520 530 540 550
PSPLIAIDEN KICCKPLVEA VKITKSVADA IEAQDFDKAM SLRDHEFKEH
560 570 580 590 600
LANFMAMNSA VHEKPTLPVE KRKKIAIVNI GAPAGGMNSA VYAMATYCMS
610 620 630 640 650
RGHTPYAIHN GFAGLARHES VKSMNWIDIE GWNSIGGSEI GTNRQTPEDT
660 670 680 690 700
DIGMIAHYFE KYQFDGLIIV GGFEAFVSLE QLERSRAMYP AFRIPMVLIP
710 720 730 740 750
ATISNNVPGT EYSLGADTCL NSLMEYCDIV KQSASATRGT AFVVDVQGGN
760 770 780 790 800
SGYIATYAAL ISGAQASYVP EEGINLSQLE NDINSLRESF CIEREMTKSG
810 820 830 840 850
KLIIKSSNAS KVLTPHVLAD IINAESHGEF DTKTAIPGHV QQGGLPSPID
860 870 880 890 900
RARGDRFAIK AVQFIEDHAD DLSGLRYSLE FPDDDKKIMN TAAVLGVKSS
910 920 930 940
HLKFTSIRHL YDFETELGRR MPKKIHWSNT REIADRLVGR TRI
Length:943
Mass (Da):103,478
Last modified:July 28, 2009 - v1
Checksum:iF1E85D43802C9B26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG692401 Genomic DNA. Translation: EER31385.1.
RefSeqiXP_002550817.1. XM_002550771.1.

Genome annotation databases

EnsemblFungiiEER31385; EER31385; CTRG_05115.
GeneIDi8299271.
KEGGictp:CTRG_05115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG692401 Genomic DNA. Translation: EER31385.1.
RefSeqiXP_002550817.1. XM_002550771.1.

3D structure databases

ProteinModelPortaliC5MGC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi294747.XP_002550817.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEER31385; EER31385; CTRG_05115.
GeneIDi8299271.
KEGGictp:CTRG_05115.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
KOiK00850.
OrthoDBiEOG092C0LOE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC5MGC2_CANTT
AccessioniPrimary (citable) accession number: C5MGC2
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 30, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.