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Protein

Methionine aminopeptidase 2-2

Gene

BDBG_07768

Organism
Blastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217SubstrateUniRule annotation1
Metal bindingi238Divalent metal cation 1UniRule annotation1
Metal bindingi249Divalent metal cation 1UniRule annotation1
Metal bindingi249Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi318Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei326SubstrateUniRule annotation1
Metal bindingi351Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi446Divalent metal cation 1UniRule annotation1
Metal bindingi446Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2-2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2-2UniRule annotation
Short name:
MetAP 2-2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
ORF Names:BDBG_07768
OrganismiBlastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)
Taxonomic identifieri559298 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeBlastomyces
Proteomesi
  • UP000002038 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004076171 – 465Methionine aminopeptidase 2-2Add BLAST465

Interactioni

Protein-protein interaction databases

STRINGi559298.XP_002621929.1.

Structurei

3D structure databases

ProteinModelPortaliC5JYZ5.
SMRiC5JYZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi72 – 85Lys-richAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775. Eukaryota.
COG0024. LUCA.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiView protein in InterPro
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
PfamiView protein in Pfam
PF00557. Peptidase_M24. 1 hit.
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiView protein in PROSITE
PS01202. MAP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

C5JYZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSKTPNDHR RGPNVESSPH AAIDTINPPK HAAASGLLHG PLEGETEDGE
60 70 80 90 100
DEDDDKTGAD LKSVGQLNNS TKKKNKRKKN KKKKKTLLGG LQTTPPRVAL
110 120 130 140 150
SSIFYNQRYP EAEIVGYTTN NDNLQRITAE EFRHLCVVND MDDEFLNDYR
160 170 180 190 200
KAAEVHRQVR QYVQTITKPG IAMSQLAQEI EDGVRALTDH QGIETGDALK
210 220 230 240 250
AGMAFPTGLC LNNIGAHWTP NPGAKEVILQ YDDVLKVDFG VHVNGRIVDS
260 270 280 290 300
AYTMAFNPVY DDLLTAVKAA TNTGLKEAGI DARIDCISEA IQEVMESYEV
310 320 330 340 350
ELNRKIIPVK AVRNITGHNI LRYKIHGDKQ VPFVKTHTNQ RMEEGDIFAI
360 370 380 390 400
ETFGSTGKAY LDDDIGIYGY FCDEHASAAG LHHSSAKSLL KTIKDNFGTL
410 420 430 440 450
VFSRRYLERL GVKSYHLGMR SLVSKGIVQS YAPLVDVPGS YVAQFEHTVL
460
LRPNCKEVIS RGDDY
Length:465
Mass (Da):51,512
Last modified:May 10, 2017 - v2
Checksum:i3B2F0F4E2556263B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG657467 Genomic DNA. Translation: OAT12429.1.
RefSeqiXP_002621929.1. XM_002621883.1.

Genome annotation databases

EnsemblFungiiEEQ73554; EEQ73554; BDBG_07768.
GeneIDi8502192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG657467 Genomic DNA. Translation: OAT12429.1.
RefSeqiXP_002621929.1. XM_002621883.1.

3D structure databases

ProteinModelPortaliC5JYZ5.
SMRiC5JYZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi559298.XP_002621929.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEEQ73554; EEQ73554; BDBG_07768.
GeneIDi8502192.

Phylogenomic databases

eggNOGiKOG2775. Eukaryota.
COG0024. LUCA.
OrthoDBiEOG092C3NQP.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiView protein in InterPro
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
PfamiView protein in Pfam
PF00557. Peptidase_M24. 1 hit.
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiView protein in PROSITE
PS01202. MAP_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAP22_BLAGS
AccessioniPrimary (citable) accession number: C5JYZ5
Secondary accession number(s): A0A179UYY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 10, 2017
Last modified: May 10, 2017
This is version 50 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.