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C5J703

- C5J703_MYCCR

UniProt

C5J703 - C5J703_MYCCR

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742ATPUniRule annotation
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:MCJ_005670Imported
    OrganismiMycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147)Imported
    Taxonomic identifieri572263 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000001491: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi45361.MCJ_005670.

    Structurei

    3D structure databases

    ProteinModelPortaliC5J703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
    Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
    Regioni251 – 2544Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGIYRGYK.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C5J703-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRKIGILTS GGDSPGMNSA LKAVAKAAIS YNIEPYFIFN GYSGIINKNI    50
    KSAKEFGYDT IGSLGGTVIG STRFVEFREL EVQKKAKEIL DELGIESLVV 100
    IGGDGTYKGG YKLHKLGVKV IGLPGTIDND IQFTDYTIGF DTALNTIVES 150
    IDKLRDTAAS HKRVFLVEVM GRHCQDLALY SGIATGSEII ITNTFRPSDE 200
    EIVEIVKQQF AKGKSNVIIN VSEYVIDDLK ALAKYIESST GFVTKHVELA 250
    HLQRGGRPSA FERILAAQMG VYAVELIVNG ESGFAVGHLN GKIEKVPLKE 300
    VFTTKNANSS FLVEKYNKIN QT 322
    Length:322
    Mass (Da):35,081
    Last modified:July 28, 2009 - v1
    Checksum:iB8C74ED7174B86CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM864216 Genomic DNA. Translation: CAT05266.1.
    RefSeqiWP_012751759.1. NC_012806.1.
    YP_002961069.1. NC_012806.1.

    Genome annotation databases

    EnsemblBacteriaiCAT05266; CAT05266; MCJ_005670.
    GeneIDi7989360.
    KEGGimco:MCJ_005670.
    PATRICi20007335. VBIMycCon56923_0543.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FM864216 Genomic DNA. Translation: CAT05266.1 .
    RefSeqi WP_012751759.1. NC_012806.1.
    YP_002961069.1. NC_012806.1.

    3D structure databases

    ProteinModelPortali C5J703.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 45361.MCJ_005670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAT05266 ; CAT05266 ; MCJ_005670 .
    GeneIDi 7989360.
    KEGGi mco:MCJ_005670.
    PATRICi 20007335. VBIMycCon56923_0543.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GIYRGYK.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Mycoplasma conjunctivae genome sequencing, annotation and analysis."
      Calderon-Copete S.P., Wigger G., Wunderlin C., Schmidheini T., Frey J., Quail M.A., Falquet L.
      BMC Bioinformatics 10:S7-S7(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25834 / HRC/581 / NCTC 10147Imported.

    Entry informationi

    Entry nameiC5J703_MYCCR
    AccessioniPrimary (citable) accession number: C5J703
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3