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C5J703

- C5J703_MYCCR

UniProt

C5J703 - C5J703_MYCCR

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Active sitei128 – 1281Proton acceptorUniRule annotation
Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATPUniRule annotation
Nucleotide bindingi103 – 1064ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:MCJ_005670Imported
OrganismiMycoplasma conjunctivae (strain ATCC 25834 / HRC/581 / NCTC 10147)Imported
Taxonomic identifieri572263 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001491: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi45361.MCJ_005670.

Structurei

3D structure databases

ProteinModelPortaliC5J703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni170 – 1723Substrate bindingUniRule annotation
Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni251 – 2544Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiGIYRGYK.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5J703-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRKIGILTS GGDSPGMNSA LKAVAKAAIS YNIEPYFIFN GYSGIINKNI
60 70 80 90 100
KSAKEFGYDT IGSLGGTVIG STRFVEFREL EVQKKAKEIL DELGIESLVV
110 120 130 140 150
IGGDGTYKGG YKLHKLGVKV IGLPGTIDND IQFTDYTIGF DTALNTIVES
160 170 180 190 200
IDKLRDTAAS HKRVFLVEVM GRHCQDLALY SGIATGSEII ITNTFRPSDE
210 220 230 240 250
EIVEIVKQQF AKGKSNVIIN VSEYVIDDLK ALAKYIESST GFVTKHVELA
260 270 280 290 300
HLQRGGRPSA FERILAAQMG VYAVELIVNG ESGFAVGHLN GKIEKVPLKE
310 320
VFTTKNANSS FLVEKYNKIN QT
Length:322
Mass (Da):35,081
Last modified:July 28, 2009 - v1
Checksum:iB8C74ED7174B86CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM864216 Genomic DNA. Translation: CAT05266.1.
RefSeqiWP_012751759.1. NC_012806.1.
YP_002961069.1. NC_012806.1.

Genome annotation databases

EnsemblBacteriaiCAT05266; CAT05266; MCJ_005670.
GeneIDi7989360.
KEGGimco:MCJ_005670.
PATRICi20007335. VBIMycCon56923_0543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FM864216 Genomic DNA. Translation: CAT05266.1 .
RefSeqi WP_012751759.1. NC_012806.1.
YP_002961069.1. NC_012806.1.

3D structure databases

ProteinModelPortali C5J703.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 45361.MCJ_005670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAT05266 ; CAT05266 ; MCJ_005670 .
GeneIDi 7989360.
KEGGi mco:MCJ_005670.
PATRICi 20007335. VBIMycCon56923_0543.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi GIYRGYK.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Mycoplasma conjunctivae genome sequencing, annotation and analysis."
    Calderon-Copete S.P., Wigger G., Wunderlin C., Schmidheini T., Frey J., Quail M.A., Falquet L.
    BMC Bioinformatics 10:S7-S7(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25834 / HRC/581 / NCTC 10147Imported.

Entry informationi

Entry nameiC5J703_MYCCR
AccessioniPrimary (citable) accession number: C5J703
Entry historyi
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3