C5EBA8 (C5EBA8_BIFLI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008

Gene names

Name:	thyA HAMAP MF_00008
ORF Names:	BLIG_01626 EMBL EEQ55302.1

Organism

Bifidobacterium longum subsp. infantis CCUG 52486 EMBL EEQ55302.1

Taxonomic identifier

537937 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Subunit structure	Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. RuleBase RU004150 Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
Biological process	Nucleotide biosynthesis SAAS SAAS023451 HAMAP MF_00008 RuleBase RU000500
Cellular component	Cytoplasm HAMAP MF_00008
Molecular function	Methyltransferase HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451 Transferase
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	149	1	By similarity HAMAP MF_00008

Sequences

	Sequence		Length	Mass (Da)
	C5EBA8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: E8BDC2C4B8B9DE70	FASTA	266	30,689
	10 20 30 40 50 60 MPYEDLVRKI LTEGTLKSDR TGTGTISLFG QQMRFDLSKY FPLLTTKTVF FKGLAYELLW 70 80 90 100 110 120 FLKGSSNINW LLEHNVHIWD EWADENGDLG PVYGVQWRSW PAPTPEDPNR TIDQISNVLD 130 140 150 160 170 180 LIKHHPDSRR MIVSAWNPAE VEKMALPPCH ALFQFYVADD KLSCQLYQRS CDMFLGVPFN 190 200 210 220 230 240 IASYSLLTMM MAQQAGLKPG EFVWTGGDCH VYDNHVDQFL EQLGRKPYPY PTIEIRKADS 250 260 LFDYQYEDFK VVGYQHHPTI KAPVAV

References

[1]

"Annotation of Bifidobacterium longum subsp. infantis CCUG 52486." The Broad Institute Genome Sequencing Platform Gougoulias C., Tuohy K.M., Gibson G.R., Ward D., Mehta T., Young S., Jaffe D., Gnerre S., Berlin A., Heiman D., Hepburn T., Shea T., Sykes S., Alvarado L., Kodira C., Borodovsky M., Lander E., Galagan J., Nusbaum C., Birren B. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: CCUG 52486 EMBL EEQ55302.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS990240 Genomic DNA. Translation: EEQ55302.1.
3D structure databases
ProteinModelPortal	C5EBA8.
SMR	C5EBA8. Positions 3-266.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	26497058. VBIBifLon94805_1201.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C5EBA8_BIFLI

Accession

Primary (citable) accession number: C5EBA8

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	December 14, 2011
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)