C5DT72 (KEX1_ZYGRC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone-processing carboxypeptidase KEX1
EC=3.4.16.6

Alternative name(s):
Carboxypeptidase D

Gene names

Name:	KEX1
Ordered Locus Names:	ZYRO0C06006g

Organism

Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) (Candida mogii) [Complete proteome]

Taxonomic identifier

559307 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces › Zygosaccharomyces rouxii

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.
Catalytic activity	Preferential release of a C-terminal arginine or lysine residue.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 684	668	Pheromone-processing carboxypeptidase KEX1		PRO_0000411957
Regions
	Topological domain	17 – 588	572	Lumenal Potential
	Transmembrane	589 – 609	21	Helical; Potential
	Topological domain	610 – 684	75	Cytoplasmic Potential
	Compositional bias	514 – 521	8	Poly-Glu
Sites
	Active site	181	1	By similarity
	Active site	390	1	By similarity
	Active site	454	1	By similarity
Amino acid modifications
	Glycosylation	443	1	N-linked (GlcNAc...) Potential
	Glycosylation	451	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C5DT72 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: BDC4CF4CFF0A311E	FASTA	684	78,284
	10 20 30 40 50 60 MRFWYFSAIL WVVCQALPSK KQYSVAPELL PGLSEIEDKS TIPEMYAGHM PLAQSNSDDK 70 80 90 100 110 120 DEIDYFFWKF SRPDKVLNTL IIWLNGGPGC SSMDGALVEN GPFRVNKDLK LVVNEGSWHT 130 140 150 160 170 180 RADMLYVDQP VNTGFSVSNS KEKKYDEDLT LTTQHFMDFL ESYFKVFPDD QFKDLIIAGE 190 200 210 220 230 240 SYSGQYVPFL AEAIQKRNAE TSDDLAKYNL RGILVGNGWM DPDTQSLAYL PFALSKGLID 250 260 270 280 290 300 QNNPHFSTLL RQQEKCQDRI ISRNPNEHQP FQYEECENIL QSILTATRDV SADTPSNQVC 310 320 330 340 350 360 MNIYSYNLRD SYPACGSNWP DEVLHVPGFF DRPGILEALN LDPSKVPQWK ECNLEVYYHL 370 380 390 400 410 420 KNRKAVPSVR KLPALLDSGL KVILYNGEMD LLCNERGVLD MIDKLQWGGA KGFSSKTKEY 430 440 450 460 470 480 DWNYRDFETN TDHIAGNVLH DRNLTYISVH NASHMVPNDK SLYSRGVVDI YLDDIFLEEL 490 500 510 520 530 540 HGKDVLVTTS EKDMDDFDNS KLGVLGITDG KPSEEEELEE EFDQYVDELE EGESESGLLD 550 560 570 580 590 600 DDKEDETVGA TDQNDDKNKG GEDKPNENPD KEKEEQDRQR KRRKGTFKIF GITILVVLTL 610 620 630 640 650 660 GSFVFYIYIR KHTNKTRAIL IDPSRRQHDS QNKRVSWADD LEHGYDFETD QSQPRSGQSA 670 680 PKKNGSYTRV PNTELDESFE LENL

References

[1]

"Comparative genomics of protoploid Saccharomycetaceae." The Genolevures Consortium Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V., Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C., Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V., Vacherie B., Val M.-E. , Fulton R.S., Minx P., Wilson R., Durrens P., Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L., Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V., Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I., Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A., Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L., Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E. Genome Res. 19:1696-1709(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928175 Genomic DNA. Translation: CAR26983.1.
RefSeq	XP_002495916.1. XM_002495871.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	559307.ZYRO0C06006g.
Protein family/group databases
MEROPS	S10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8203117.
KEGG	zro:ZYRO0C06006g.
Phylogenomic databases
eggNOG	COG2939.
HOGENOM	HOG000208879.
KO	K01288.
OrthoDB	EOG44QX8Z.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. 1 hit. PS00131. CARBOXYPEPT_SER_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX1_ZYGRC

Accession

Primary (citable) accession number: C5DT72

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program