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Protein

Pheromone-processing carboxypeptidase KEX1

Gene

KEX1

Organism
Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death (By similarity).By similarity

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei214 – 2141By similarity
Active sitei418 – 4181By similarity
Active sitei482 – 4821By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Apoptosis

Protein family/group databases

MEROPSiS10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
Alternative name(s):
Carboxypeptidase D
Gene namesi
Name:KEX1
Ordered Locus Names:KLTH0G01980g
OrganismiLachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Taxonomic identifieri559295 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea
ProteomesiUP000002036 Componenti: Chromosome G

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini48 – 655608LumenalSequence AnalysisAdd
BLAST
Transmembranei656 – 67621HelicalSequence AnalysisAdd
BLAST
Topological domaini677 – 74569CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4747Sequence AnalysisAdd
BLAST
Chaini48 – 745698Pheromone-processing carboxypeptidase KEX1PRO_0000411922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi479 – 4791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi552 – 5521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi559295.KLTH0G01980g.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi497 – 648152Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2939.
HOGENOMiHOG000208879.
InParanoidiC5DLM9.
KOiK01288.
OrthoDBiEOG7TJ3SJ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5DLM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHFKYRNQT TDICNAAALD AFAMYSNYSV PLALLFALLL SFQTARALKA
60 70 80 90 100
ADEYAVSPDL IPGLSQAEDR ALVPTMHAGH IPLDENNDEH TKNYFFWKFH
110 120 130 140 150
DESGSASGPA RDTLIFWLNG GPGCSSMDGA LMESGALRID SDGKAYLNRG
160 170 180 190 200
GWHTRGDIVF VDQPAGTGFS TVASDNDYDN DLKVVSEHFV AFLRNYFQVF
210 220 230 240 250
PDDAGKQVVF AGESYAGQFI PYFARAVLDQ DEVQVNLQAL LIGNGWIDPN
260 270 280 290 300
QQSLYYIPFA VEKGLITQDD PSFSYLLKQQ ENCQNKINSK ENDRFSFKEC
310 320 330 340 350
ENILNNLLEV TRKIKDSEGK KVPSDQQCIN MYDLRLKDSY PSCGMNWPQD
360 370 380 390 400
LPNLGKFFGT EGVLEALHLD PEHVSQWHEC DDKVSNYLKN PDSRASAAIL
410 420 430 440 450
PGLLEAGLEV MLFNGDQDII CNNMGVEAVI SQMSWGGETG FSENVQLYDW
460 470 480 490 500
VYRSPENSEI TPAGFVKYDR NLTFMSVYNA SHMVPFDNAL VSRGVVDLFL
510 520 530 540 550
NDVDLVQIDG RDTLITDDVN KGKDGDASET DDTTELDCEG KDKLSEECKQ
560 570 580 590 600
LNASKGQNGE SDKDEGEKGN EEDDNDDTED GKKDAGDEKD DGEKDDDEKD
610 620 630 640 650
GDEKDGDEKD EEDDDDKDEN EKDEEDDDKD GDKPEGKNND GAEDEDDDHN
660 670 680 690 700
SGSHLAVTMI CLLVSGTIIG GLYFTFRDRF RPRLRAILVD PTNRSEASKK
710 720 730 740
TVSWAADLEQ DAADLSNPEN GAKKKGPYTS VPTQESRDSF ELDNL
Length:745
Mass (Da):82,855
Last modified:July 28, 2009 - v1
Checksum:iECAC755742FE5B2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928171 Genomic DNA. Translation: CAR24690.1.
RefSeqiXP_002555127.1. XM_002555081.1.

Genome annotation databases

GeneIDi8293386.
KEGGilth:KLTH0G01980g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928171 Genomic DNA. Translation: CAR24690.1.
RefSeqiXP_002555127.1. XM_002555081.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi559295.KLTH0G01980g.

Protein family/group databases

MEROPSiS10.007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8293386.
KEGGilth:KLTH0G01980g.

Phylogenomic databases

eggNOGiCOG2939.
HOGENOMiHOG000208879.
InParanoidiC5DLM9.
KOiK01288.
OrthoDBiEOG7TJ3SJ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomics of protoploid Saccharomycetaceae."
    The Genolevures Consortium
    Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V., Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C., Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V., Vacherie B., Val M.-E.
    , Fulton R.S., Minx P., Wilson R., Durrens P., Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L., Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V., Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I., Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A., Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L., Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.
    Genome Res. 19:1696-1709(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 56472 / CBS 6340 / NRRL Y-8284.

Entry informationi

Entry nameiKEX1_LACTC
AccessioniPrimary (citable) accession number: C5DLM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 28, 2009
Last modified: January 7, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.