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C5DGZ5

- LIPA_LACTC

UniProt

C5DGZ5 - LIPA_LACTC

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Protein

Lipoyl synthase, mitochondrial

Gene
KLTH0D09548g
Organism
Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi112 – 1121Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi118 – 1181Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi145 – 1451Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Ordered Locus Names:KLTH0D09548g
OrganismiLachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Taxonomic identifieri559295 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea
ProteomesiUP000002036: Chromosome D

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion Reviewed predictionAdd
BLAST
Chaini25 – 371347Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398270Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi559295.KLTH0D09548g.

Structurei

3D structure databases

ProteinModelPortaliC5DGZ5.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C5DGZ5-1 [UniParc]FASTAAdd to Basket

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MLSRFKCSRL QLQKRAISVT KATTTTASQP KRRRTTTFSD ALNKGPSFED    50
FVSGKAAKFT LDPLQQARSN IEEAKRLPRW LKVPIPKGTN YHKLKKDVRE 100
LKLSTVCEEA KCPNISECWG GGDSSKATAT IMLLGDTCTR GCRFCSVKTN 150
RAPSKPDPAE PENTAEAISR WGLGYVVLTT VDRDDLADGG AHHLAETVCR 200
IKQKAPKTLV ETLSGDFRGD LEMVKVMAQS GLDVYAHNLE TVKDLTPHVR 250
DRRATYEQSL SVLNQAKKTV PTLITKTSLM LGLGETDEQV LQTLQDLRAI 300
GCDVVTFGQY MRPTKRHMKV VEYVKPEKFD YWRDKALELG FLYCASGPLV 350
RSSYKAGEAF IENVLRKRAN N 371
Length:371
Mass (Da):41,451
Last modified:July 28, 2009 - v1
Checksum:i54736E8D8C575ECB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928168 Genomic DNA. Translation: CAR22687.1.
RefSeqiXP_002553125.1. XM_002553079.1.

Genome annotation databases

GeneIDi8295363.
KEGGilth:KLTH0D09548g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928168 Genomic DNA. Translation: CAR22687.1 .
RefSeqi XP_002553125.1. XM_002553079.1.

3D structure databases

ProteinModelPortali C5DGZ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 559295.KLTH0D09548g.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8295363.
KEGGi lth:KLTH0D09548g.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of protoploid Saccharomycetaceae."
    The Genolevures Consortium
    Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V., Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C., Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V., Vacherie B., Val M.-E.
    , Fulton R.S., Minx P., Wilson R., Durrens P., Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L., Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V., Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I., Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A., Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L., Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.
    Genome Res. 19:1696-1709(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 56472 / CBS 6340 / NRRL Y-8284.

Entry informationi

Entry nameiLIPA_LACTC
AccessioniPrimary (citable) accession number: C5DGZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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