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C5DE35

- MAP2_LACTC

UniProt

C5DE35 - MAP2_LACTC

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Protein

Methionine aminopeptidase 2

Gene

MAP2

Organism
Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei172 – 1721SubstrateUniRule annotation
Metal bindingi192 – 1921Divalent metal cation 1UniRule annotation
Metal bindingi203 – 2031Divalent metal cation 1UniRule annotation
Metal bindingi203 – 2031Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi272 – 2721Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei280 – 2801SubstrateUniRule annotation
Metal bindingi305 – 3051Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi401 – 4011Divalent metal cation 1UniRule annotation
Metal bindingi401 – 4011Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:MAP2UniRule annotation
Ordered Locus Names:KLTH0C05962g
OrganismiLachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Taxonomic identifieri559295 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea
ProteomesiUP000002036: Chromosome C

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Methionine aminopeptidase 2PRO_0000407654Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi559295.KLTH0C05962g.

Structurei

3D structure databases

ProteinModelPortaliC5DE35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 489Poly-Lys

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000226278.
InParanoidiC5DE35.
KOiK01265.
OrthoDBiEOG7BGHW3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C5DE35-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDAIAKDAV NTSSEKEPVS ATPELKTSGS PDAAVSSGDK KKKKKKKKTN
60 70 80 90 100
NIKNIALLYP DEKYPEGEWM DYHQDFNLQR TTDEEKRYLT RDAENQQRWN
110 120 130 140 150
DMRKGAEIHR RVRKNLQNKL KPDMTLTEVV NIVENATRKF TGVDENGDHV
160 170 180 190 200
DRPKSQGVGF PTGVSLNHCA AHFTPNAGDQ TVLRYEDVMK VDIGVQVNGY
210 220 230 240 250
IVDSAWTVAF DPKYDNLLQA VREATNTGVR EAGIDVRLTD IGEAIQEVME
260 270 280 290 300
SYEVELNGKT HQVKPCRNLC GHNIAPYRIH GGKSVPIVKN GDQTKMEEGE
310 320 330 340 350
HFAIETFGST GRGFVVPGGE VSHYAKSAEA DGLPAPSLSR AKSLLKTIDE
360 370 380 390 400
NFGTLPFCRR YLDRLGEDKY LFALNSLVKH GIVQDYPPLN DVQGSYTAQF
410 420
EHTILLHPHR KEVVSRGEDY
Length:420
Mass (Da):47,002
Last modified:July 28, 2009 - v1
Checksum:i9F4BB358F38D7178
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928167 Genomic DNA. Translation: CAR22046.1.
RefSeqiXP_002552484.1. XM_002552438.1.

Genome annotation databases

GeneIDi8291352.
KEGGilth:KLTH0C05962g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU928167 Genomic DNA. Translation: CAR22046.1 .
RefSeqi XP_002552484.1. XM_002552438.1.

3D structure databases

ProteinModelPortali C5DE35.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 559295.KLTH0C05962g.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8291352.
KEGGi lth:KLTH0C05962g.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000226278.
InParanoidi C5DE35.
KOi K01265.
OrthoDBi EOG7BGHW3.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPi MF_03175. MetAP_2_euk.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
PROSITEi PS01202. MAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of protoploid Saccharomycetaceae."
    The Genolevures Consortium
    Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V., Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C., Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V., Vacherie B., Val M.-E.
    , Fulton R.S., Minx P., Wilson R., Durrens P., Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L., Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V., Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I., Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A., Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L., Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.
    Genome Res. 19:1696-1709(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 56472 / CBS 6340 / NRRL Y-8284.

Entry informationi

Entry nameiMAP2_LACTC
AccessioniPrimary (citable) accession number: C5DE35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3