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C5D9D5 (PNP_GEOSW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase

EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:GWCH70_1161
OrganismGeobacillus sp. (strain WCH70) [Complete proteome] [HAMAP]
Taxonomic identifier471223 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01595

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 712712Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595
PRO_1000215661

Regions

Domain554 – 61360KH
Domain623 – 69169S1 motif

Sites

Metal binding4871Magnesium By similarity
Metal binding4931Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
C5D9D5 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: FDAE9FE8F54103B7

FASTA71278,909
        10         20         30         40         50         60 
MEQEKRIFSI DWAGRPLVVE IGQLAKQANG AVLVRYGDTV VLNTATASKE AKNVDFFPLT 

        70         80         90        100        110        120 
VNYEERLYAV GKIPGGFIKR EGRPSEKAIL ASRLIDRPIR PLFAEGFRNE VQIVSMVMSV 

       130        140        150        160        170        180 
DQDCSPEMAA LFGASLALTI SDIPFEGPIA GVTVGRVDGE FVINPTVEQS EKSDIHLVVA 

       190        200        210        220        230        240 
GTKDAINMVE AGADEVPEEV ILEAIMFGHE EVKRLIAFQE EIAAQVGKEK MEVVLYELDP 

       250        260        270        280        290        300 
QLEAEIRQLA EEDIKRAVQV PEKLARDAAI EEVKASVIAK YEEQEADEET LKQVNEILHK 

       310        320        330        340        350        360 
LVKEEVRRLI TEEKIRPDGR KIDEIRPLSS EVGVLPRTHG SGLFTRGQTQ VLSVCTLGAL 

       370        380        390        400        410        420 
GDVQILDGLG IEETKRFMHH YNFPPFSVGE TGPMRGPGRR EIGHGALGER ALEPVVPSEK 

       430        440        450        460        470        480 
EFPYTIRLVS EVLESNGSTS QASICASTLA MMDAGVPIKA PVAGIAMGLV KNDDNYTILT 

       490        500        510        520        530        540 
DIQGIEDHLG DMDFKVAGTA KGVTALQMDI KIKGLSREIL EEALQQAKKG RMEILEHMMQ 

       550        560        570        580        590        600 
TIREPRKELS KYAPKILTMQ INPEKIREVI GPSGKQINKI IDETGVKIDI EQDGTIFISS 

       610        620        630        640        650        660 
VNEAMNQKAK QIIEDIVREV EVGQIYLGKV KRIEKFGAFV ELFNGKDGLV HISELAEERV 

       670        680        690        700        710 
GRVEDVVSIG DEILVKVMEI DKQGRVNLSR KAVLRDKKEK KGKRPERHRM KP 

« Hide

References

[1]"Complete sequence of chromosome of Geopacillus sp. WCH70."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Brumm P., Mead D.A., Richardson P.
Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: WCH70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001638 Genomic DNA. Translation: ACS24021.1.
RefSeqYP_002949287.1. NC_012793.1.

3D structure databases

ProteinModelPortalC5D9D5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING471223.GWCH70_1161.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS24021; ACS24021; GWCH70_1161.
GeneID7977639.
KEGGgwc:GWCH70_1161.
PATRIC21971246. VBIGeoSp101709_1238.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHOG000218327.
KOK00962.
OMAPRWDWVA.
OrthoDBEOG6WT8CC.

Enzyme and pathway databases

BioCycGSP471223:GH2C-1245-MONOMER.

Family and domain databases

Gene3D1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPMF_01595. PNPase.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERPTHR11252. PTHR11252. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsTIGR03591. polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_GEOSW
AccessionPrimary (citable) accession number: C5D9D5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families