C5D9D5 (PNP_GEOSW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8

Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	GWCH70_1161

Organism

Geobacillus sp. (strain WCH70) [Complete proteome] [HAMAP]

Taxonomic identifier

471223 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: HAMAP polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595		PRO_1000215661
Regions
	Domain	554 – 613	60	KH
	Domain	623 – 691	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	C5D9D5 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: FDAE9FE8F54103B7	FASTA	712	78,909
	10 20 30 40 50 60 MEQEKRIFSI DWAGRPLVVE IGQLAKQANG AVLVRYGDTV VLNTATASKE AKNVDFFPLT 70 80 90 100 110 120 VNYEERLYAV GKIPGGFIKR EGRPSEKAIL ASRLIDRPIR PLFAEGFRNE VQIVSMVMSV 130 140 150 160 170 180 DQDCSPEMAA LFGASLALTI SDIPFEGPIA GVTVGRVDGE FVINPTVEQS EKSDIHLVVA 190 200 210 220 230 240 GTKDAINMVE AGADEVPEEV ILEAIMFGHE EVKRLIAFQE EIAAQVGKEK MEVVLYELDP 250 260 270 280 290 300 QLEAEIRQLA EEDIKRAVQV PEKLARDAAI EEVKASVIAK YEEQEADEET LKQVNEILHK 310 320 330 340 350 360 LVKEEVRRLI TEEKIRPDGR KIDEIRPLSS EVGVLPRTHG SGLFTRGQTQ VLSVCTLGAL 370 380 390 400 410 420 GDVQILDGLG IEETKRFMHH YNFPPFSVGE TGPMRGPGRR EIGHGALGER ALEPVVPSEK 430 440 450 460 470 480 EFPYTIRLVS EVLESNGSTS QASICASTLA MMDAGVPIKA PVAGIAMGLV KNDDNYTILT 490 500 510 520 530 540 DIQGIEDHLG DMDFKVAGTA KGVTALQMDI KIKGLSREIL EEALQQAKKG RMEILEHMMQ 550 560 570 580 590 600 TIREPRKELS KYAPKILTMQ INPEKIREVI GPSGKQINKI IDETGVKIDI EQDGTIFISS 610 620 630 640 650 660 VNEAMNQKAK QIIEDIVREV EVGQIYLGKV KRIEKFGAFV ELFNGKDGLV HISELAEERV 670 680 690 700 710 GRVEDVVSIG DEILVKVMEI DKQGRVNLSR KAVLRDKKEK KGKRPERHRM KP

References

[1]

"Complete sequence of chromosome of Geopacillus sp. WCH70." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Brumm P., Mead D.A., Richardson P. Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WCH70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001638 Genomic DNA. Translation: ACS24021.1.
RefSeq	YP_002949287.1. NC_012793.1.
3D structure databases
ProteinModelPortal	C5D9D5.
ModBase	Search...
Protein-protein interaction databases
STRING	471223.GWCH70_1161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACS24021; ACS24021; GWCH70_1161.
GeneID	7977639.
KEGG	gwc:GWCH70_1161.
PATRIC	21971246. VBIGeoSp101709_1238.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1185.
HOGENOM	HOG000218327.
KO	K00962.
OMA	YTMRVVS.
ProtClustDB	PRK11824.
Enzyme and pathway databases
BioCyc	GSP471223:GH2C-1279-MONOMER.
Family and domain databases
Gene3D	1.10.10.400. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_01595. PNPase.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR022967. RNA-binding_domain_S1. [Graphical view]
PANTHER	PTHR11252. PTHR11252. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF46915. 3_ExoRNase. 1 hit. SSF55666. 3_ExoRNase. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR03591. polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_GEOSW

Accession

Primary (citable) accession number: C5D9D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program